Sampo Tuukkanen

Carbon nanotubes as electrodes for dielectrophoresis of DNA

Dielectrophoresis can potentially be used as an efficient trapping tool in the fabrication of molecular devices. For nanoscale objects, however, the Brownian motion poses a challenge. We show that the use of carbon nanotube electrodes makes it possible to apply relatively low trapping voltages and still achieve high enough field gradients for trapping nanoscale objects, e.g., single molecules. We compare the efficiency and other characteristics of dielectrophoresis between carbon nanotube electrodes and lithographically fabricated metallic electrodes, in the case of trapping nanoscale DNA molecules. The results are analyzed using finite element method simulations and reveal information about the frequency-dependent polarizability of DNA.

Preparation of Supercapacitors on Flexible Substrates with Electrodeposited PEDOT/Graphene Composites

Composite films consisting of poly(3,4-ethylenedioxythiophene) (PEDOT) and graphene oxide (GO) were electrochemically polymerized by electrooxidation of EDOT in ionic liquid (BMIMBF4) onto flexible electrode substrates. Two polymerization approaches were compared, and the cyclic voltammetry (CV) method was found to be superior to potentiostatic polymerization for the growth of PEDOT/GO films. After deposition, incorporated GO was reduced to rGO by a rapid electrochemical method of repetitive cathodic potential cycling, without using any reducing reagents. The films were characterized in 3-electrode configuration in BMIMBF4. Symmetric supercapacitors with aqueous electrolyte were assembled from the composite films and characterized through cyclic voltammetry and galvanostatic discharge tests. It was shown that PEDOT/rGO composites have better capacitive properties than pure PEDOT or the unreduced composite film. The cycling stability of the supercapacitors was also tested, and the results indicate that the specific capacitance still retains well over 90% of the initial value after 2000 consecutive charging/discharging cycles. The supercapacitors were demonstrated as energy storages in a room light energy harvester with a printed organic solar cell and printed electrochromic display. The results are promising for the development of energy-autonomous, low-power, and disposable electronics.

Dielectrophoresis of nanoscale double-stranded DNA and humidity effects on its electrical conductivity

The dielectrophoresis method for trapping and attaching nanoscale double-stranded DNA between nanoelectrodes was developed. The method gives a high yield of trapping single or a few molecules only which enables transport measurements at the single molecule level. Electrical conductivity of individual 140-nm-long DNA molecules was measured, showing insulating behavior in dry conditions. In contrast, clear enhancement of conductivity was observed in moist conditions, relating to the interplay between the conformation of DNA molecules and their conductivity.

Trapping of 27 bp-8 kbp DNA and immobilization of thiol-modified DNA using dielectrophoresis

Dielectrophoretic trapping of six different DNA fragments, sizes varying from 27 to 8416 bp, has been studied using confocal microscopy. The effect of the DNA length and the size of the constriction between nanoscale fingertip electrodes on the trapping efficiency have been investigated. Using finite element method simulations in conjunction with the analysis of the experimental data, the polarizabilities of the different size DNA fragments have been calculated for different frequencies. Also the immobilization of trapped hexanethiol- and DTPA-modified 140 nm long DNA to the end of gold nanoelectrodes was experimentally quantified and the observations were supported by density functional theory calculations.

Direct conductance measurements of short single DNA molecules in dry conditions.

We present a study of electronic transport in short (12-base-pair) DNA duplexes covalently bonded (via thiol groups) to two gold electrodes obtained by a mechanically controllable break junction (MCJB) technique in dry conditions. A large number of DNA junctions have been repeatedly formed in order to obtain a conductance histogram that reveals a peak which corresponds to the conductance of a single DNA molecule. We observed that the conductivity of a DNA increases upon increasing the content of G:C base pairs in the duplex. With our method we are able to obtain a reliable value of a single DNA conductance and subsequently measure its current-voltage (I-V) characteristics. In contrast to the electronic transport measurements performed with long DNA sequences (hundreds of base pairs) where the obtained conductance values vary a lot with environmental conditions, our values obtained for the short DNA sequences are consistent with the values reported for comparable sequences in aqueous solution.

Flexible Piezoelectric Energy Harvesting Circuit With Printable Supercapacitor and Diodes

We report a flexible energy harvesting circuit fabricated by roll-to-roll compatible, solution-processable methods. The circuit incorporates a supercapacitor fabricated from a viscous carbon nanotube dispersion, printed Schottky diodes, and a piezoelectric element. Used low-temperature materials enabled component integration on poly(ethylene terephthalate) substrate. The supercapacitor was built with a paper separator and an aqueous NaCl electrolyte. Together with carbon-based electrodes, these materials translated into a disposable and environmentally safe electronic device. The energy harvested from mechanical movement was used to drive a commercial electrochromic display.

Characteristics of Piezoelectric Polymer Film Sensors With Solution-Processable Graphene-Based Electrode Materials

The sensor characteristics of piezoelectric polyvinylidenefluoride (PVDF) sensors with solution-processable electrode materials were studied. The electrodes were solutionprocessed on 28-μm-thick PVDF film. Two graphene-based printable inks, ink-jet, and screen formulated ink, were used. Sensors with evaporated metal electrodes were used as a reference to compare the properties of novel sensor structures. The sensor characteristics studied here were sensitivity, nonlinearity, hysteresis, and the effects of frequency and temperature. The sensor sensitivity measurements revealed mean sensitivities of (31.1 ± 1.4) pC/N for the reference sensors and (26.2 ± 2.2) and (21.4 ± 1.3) pC/N for the sensors with graphene-based ink-jet and screen formulated ink electrodes, respectively. The sensor characteristics of the novel sensors were found to be similar to those of the reference sensors. The new sensors are linear, hysteresis error is negligible, and the operation under changing frequency (up to 100 Hz) is rather stable. Change in ambient temperature somewhat affects the sensor sensitivities. The sensors presented here can be used in several sensing applications, e.g., in plantar pressure distribution measurements.

Toward full carbon interconnects: High conductivity of individual carbon nanotube to carbon nanotube regrowth junctions

A versatile chemical vapor deposition (CVD) based method for the fabrication and electrical measurement of individual carbon nanotube junctions was developed. ferritin or Fe particles were grafted on multiwalled carbon nanotubes (MWNTs) and used as catalysts for the subsequent growth of secondary MWNT by CVD. Junctions were then individually connected. The conductivities of the MWNTs and of the junction were measured. Statistical data show that the conductance of the MWNT-MWNT junction is similar to that of MWNT. This result paves the way for the use of carbon nanotubes as electrical interconnects in electronic applications.

One-step large-scale deposition of salt-free DNA origami nanostructures

DNA origami nanostructures have tremendous potential to serve as versatile platforms in self-assembly -based nanofabrication and in highly parallel nanoscale patterning. However, uniform deposition and reliable anchoring of DNA nanostructures often requires specific conditions, such as pre-treatment of the chosen substrate or a fine-tuned salt concentration for the deposition buffer. In addition, currently available deposition techniques are suitable merely for small scales. In this article, we exploit a spray-coating technique in order to resolve the aforementioned issues in the deposition of different 2D and 3D DNA origami nanostructures. We show that purified DNA origamis can be controllably deposited on silicon and glass substrates by the proposed method. The results are verified using either atomic force microscopy or fluorescence microscopy depending on the shape of the DNA origami. DNA origamis are successfully deposited onto untreated substrates with surface coverage of about 4 objects/mm2. Further, the DNA nanostructures maintain their shape even if the salt residues are removed from the DNA origami fabrication buffer after the folding procedure. We believe that the presented one-step spray-coating method will find use in various fields of material sciences, especially in the development of DNA biochips and in the fabrication of metamaterials and plasmonic devices through DNA metallisation.

Engineering and Characterization of Bacterial Nanocellulose Films as Low Cost and Flexible Sensor Material

Some bacterial strains such as Komagataeibacter xylinus are able to produce cellulose as an extracellular matrix. In comparison to wood-based cellulose, bacterial cellulose (BC) holds interesting properties such as biodegradability, high purity, water-holding capacity, and superior mechanical and structural properties. Aiming toward improvement in BC production titer and tailored alterations to the BC film, we engineered K. xylinus to overexpress partial and complete bacterial cellulose synthase operon that encodes activities for BC production. The changes in cell growth, end metabolite, and BC production titers from the engineered strains were compared with the wild-type K. xylinus. Although there were no significant differences between the growth of wild-type and engineered strains, the engineered K. xylinus strains demonstrated faster BC production, generating 2-4-fold higher production titer (the highest observed titer was obtained with K. xylinus-bcsABCD strain producing 4.3 ± 0.46 g/L BC in 4 days). The mechanical and structural characteristics of cellulose produced from the wild-type and engineered K. xylinus strains were analyzed with a stylus profilometer, in-house built tensile strength measurement system, a scanning electron microscope, and an X-ray diffractometer. Results from the profilometer indicated that the engineered K. xylinus strains produced thicker BC films (wild type, 5.1 μm, and engineered K. xylinus strains, 6.2-10.2 μm). Scanning electron microscope revealed no principal differences in the structure of the different type BC films. The crystallinity index of all films was high (from 88.6 to 97.5%). All BC films showed significant piezoelectric response (5.0-20 pC/N), indicating BC as a promising sensor material.