Samuel Kuna

Studies on the chronic toxicity of pyranisamine maleate (neo-antergan maleate) in animals.

Abstract 1.1. Neo-Antergan maleate (brand of pyranisamine maleate) has been administered to rats, dogs, and monkeys for varying lengths of time up to six months. The following doses appeared to be entirely safe: (a) In rats, 10 mg. per kilogram five times weekly for six months and up to 200 mg. per kilogram daily for thirty-two days; (b) in dogs, 20 mg. per kilogram five times weekly for six months; (c) in monkeys, 50 mg. per kilogram daily for thirty-five days. No toxic signs, nor any hematologic, biochemical, or pathologic abnormalities were found in the animals on these doses. 2.2. One monkey tolerated 100 mg. per kilogram daily for thirty-five days, but this proved to be a toxic level for another monkey which succumbed after five such doses. In rats, daily doses of 500 mg. per kilogram for sixty-one days and 1,000 mg. per kilogram for forty-two days proved to be toxic for some animals because many died on these dose levels. Those which survived, however, grew at a nearly normal rate. 3.3. There is no evidence that Neo-Antergan has a cumulative effect at small or moderate doses, since the drug may be administered over a long period of time with no apparent ill effects.

A Liver Function Test for Small Laboratory Animals

Summary A simple and rapid technic for performing the Rosenthal-White bromsulphalein liver function test on rats and mice is described. The test gives a roughly quantitative estimate of the degree of liver damage and since it can be repeated on the same animal it is of use in following the progress or regression of liver disturbances.