Sandeep Kumar Dash
Indian Veterinary Research Institute
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Featured researches published by Sandeep Kumar Dash.
Microbiology and Immunology | 2016
Semmannan Kalaiyarasu; Manoj Kumar; Dhanapal Senthil Kumar; Sandeep Bhatia; Sandeep Kumar Dash; Sushant Bhat; Rohit K. Khetan; S. Nagarajan
One of the major causes of death in highly pathogenic avian influenza virus (HPAIV) infection in chickens is acute induction of pro‐inflammatory cytokines (cytokine storm), which leads to severe pathology and acute mortality. DCs and respiratory tract macrophages are the major antigen presenting cells that are exposed to mucosal pathogens. We hypothesized that chicken DCs are a major target for induction of cytokine dysregulation by H5N1 HPAIV. It was found that infection of chicken peripheral blood monocyte‐derived dendritic cells (chMoDCs) with H5N1 HPAIV produces high titers of progeny virus with more rounding and cytotoxicity than with H9N2 LPAIV. Expression of maturation markers (CD40, CD80 and CD83) was weaker in both H5N1 and H9N2 groups than in a LPS control group. INF‐α, ‐β and ‐γ were significantly upregulated in the H5N1 group. Pro‐inflammatory cytokines (IL‐1β, TNF‐α and IL‐18) were highly upregulated in early mid (IL‐1), and late (IL‐6) phases of H5N1 virus infection. IL‐8 (CXCLi2) mRNA expression was significantly stronger in the H5N1 group from 6 hr of infection. TLR3, 7, 15 and 21 were upregulated 24 hr after infection by H5N1 virus compared with H9N2 virus, with maximum expression of TLR 3 mRNA. Similarly, greater H5N1 virus‐induced apoptotic cell death and cytotoxicity, as measured by terminal deoxynucleotidyl transferase‐mediated dUTP nick end labeling and lactate dehydrogenase assays, respectively, were found. Thus, both H5N1 and H9N2 viruses evade the host immune system by inducing impairment of chMoDCs maturation and enhancing cytokine dysregulation in H5N1 HPAIV‐infected cells.
Veterinary World | 2012
Sandeep Kumar Dash; Krishna Kumar; Anjana Goel; Ashok Kumar Bhatia
Veterinary World | 2013
Sarita Pachauri; Puneet Varshney; Sandeep Kumar Dash; Manoj Kumar Gupta
Medicinal Plant Research | 2013
Puneet Varshney; Sandeep Kumar Dash; Anjana Goel; Ashok Kumar Bhatia
Indian Journal of Microbiology | 2012
Sandeep Kumar Dash; Krishna Kumar; Anuj Tewari; Puneet Varshney; Anjana Goel; Ashok Kumar Bhatia
Veterinary World | 2011
Sandeep Kumar Dash; Anuj Tewari; Krishna Kumar; Anjana Goel; Ashok Kumar Bhatia
Journal of Immunology and Immunopathology | 2011
Anjana Goel; Rashmi Singh; Sandeep Kumar Dash; Deepti Gupta; Ajita Pillai; Sharad Yadav; Ashok Kumar Bhatia
Medicinal Plant Research | 2013
Puneet Varshney; Sandeep Kumar Dash; Ashok Kumar Bhatia
Journal of Animal Research | 2012
Anuj Tewari; Sandeep Kumar Dash; Beenu Jain; Ashok Kumar Bhatia
Medicinal Plants - International Journal of Phytomedicines and Related Industries | 2012
Sumit Varshney; Puneet Varshney; Sandeep Kumar Dash; Manoj Kumar Gupta; Amit Kumar; Basanti Bist; Arvind Sharma