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Dive into the research topics where Sandra A. Helquist is active.

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Featured researches published by Sandra A. Helquist.


Molecular and Cellular Biology | 2003

Requirement of Watson-Crick Hydrogen Bonding for DNA Synthesis by Yeast DNA Polymerase η

M. Todd Washington; Sandra A. Helquist; Eric T. Kool; Louise Prakash; Satya Prakash

ABSTRACT Classical high-fidelity DNA polymerases discriminate between the correct and incorrect nucleotides by using geometric constraints imposed by the tight fit of the active site with the incipient base pair. Consequently, Watson-Crick (W-C) hydrogen bonding between the bases is not required for the efficiency and accuracy of DNA synthesis by these polymerases. DNA polymerase η (Polη) is a low-fidelity enzyme able to replicate through DNA lesions. Using difluorotoluene, a nonpolar isosteric analog of thymine unable to form W-C hydrogen bonds with adenine, we found that the efficiency and accuracy of nucleotide incorporation by Polη are severely impaired. From these observations, we suggest that W-C hydrogen bonding is required for DNA synthesis by Polη; in this regard, Polη differs strikingly from classical high-fidelity DNA polymerases.


Proceedings of the National Academy of Sciences of the United States of America | 2003

High-fidelity in vivo replication of DNA base shape mimics without Watson–Crick hydrogen bonds

James C. Delaney; Paul T. Henderson; Sandra A. Helquist; Juan C. Morales; John M. Essigmann; Eric T. Kool

We report studies testing the importance of Watson–Crick hydrogen bonding, base-pair geometry, and steric effects during DNA replication in living bacterial cells. Nonpolar DNA base shape mimics of thymine and adenine (abbreviated F and Q, respectively) were introduced into Escherichia coli by insertion into a phage genome followed by transfection of the vector into bacteria. Genetic assays showed that these two base mimics were bypassed with moderate to high efficiency in the cells and with very high efficiency under damage-response (SOS induction) conditions. Under both sets of conditions, the T-shape mimic (F) encoded genetic information in the bacteria as if it were thymine, directing incorporation of adenine opposite it with high fidelity. Similarly, the A mimic (Q) directed incorporation of thymine opposite itself with high fidelity. The data establish that Watson–Crick hydrogen bonding is not necessary for high-fidelity replication of a base pair in vivo. The results suggest that recognition of DNA base shape alone serves as the most powerful determinant of fidelity during transfer of genetic information in a living organism.


Proceedings of the National Academy of Sciences of the United States of America | 2002

Artificial human telomeres from DNA nanocircle templates

Ulf M. Lindström; Ravi A. Chandrasekaran; Lucian Orbai; Sandra A. Helquist; Gregory P. Miller; Emin Oroudjev; Helen G. Hansma; Eric T. Kool

Human telomerase is a reverse-transcriptase enzyme that synthesizes the multikilobase repeating hexamer telomere sequence (TTAGGG)n at the ends of chromosomes. Here we describe a designed approach to mimicry of telomerase, in which synthetic DNA nanocircles act as essentially infinite catalytic templates for efficient synthesis of long telomeres by DNA polymerase enzymes. Results show that the combination of a nanocircle and a DNA polymerase gives a positive telomere-repeat amplification protocol assay result for telomerase activity, and similar to the natural enzyme, it is inhibited by a known telomerase inhibitor. We show that artificial telomeres can be engineered on human chromosomes by this approach. This strategy allows for the preparation of synthetic telomeres for biological and structural study of telomeres and proteins that interact with them, and it raises the possibility of telomere engineering in cells without expression of telomerase itself. Finally, the results provide direct physical support for a recently proposed rolling-circle mechanism for telomerase-independent telomere elongation.


Journal of Biological Chemistry | 2008

Importance of Hydrogen Bonding for Efficiency and Specificity of the Human Mitochondrial DNA Polymerase

Harold R. Lee; Sandra A. Helquist; Eric T. Kool; Kenneth A. Johnson

To assess the contribution to discrimination afforded by base pair hydrogen bonding during DNA replication by the human mitochondrial DNA polymerase, we examined nucleoside mimics lacking hydrogen bond forming capability but retaining the overall steric shape of the natural nucleotide. We employed oligonucleotide templates containing either a deoxyadenosine shape mimic (dQ) or a deoxythymidine shape mimic (dF). Additionally, the nucleoside triphosphate analogs difluorotoluene deoxynucleoside triphosphate, 9-methyl-1-H-imidazo[(4,5)-b]pyridine deoxyribose triphosphate, and 4-methylbenzimidazole deoxyribose triphosphate (dZTP; another dATP shape mimic) were assayed. We used pre-steady state methods to determine the kinetic parameters governing nucleotide incorporation, kpol and Kd. In general, the loss of hydrogen bonding potential led to 2–3 kcal/mol reduction in ground state binding free energy, whereas effects on the maximum rate of polymerization were quite variable, ranging from negligible (dATP:dF) to nearly 4 kcal/mol (dZTP:dT). Although we observed only a 46-fold reduction in discrimination when dF was present in the template, there was a complete elimination of discrimination when dQ was present in the template. Our data with dF indicate that hydrogen bonding contributes 2.2 kcal/mol toward the efficiency of incorporation, whereas data with dQ (which may overestimate the effect due to poor steric mimicry) suggest a contribution of up to 6.8 kcal/mol. Taken together, the data suggest that sterics are necessary but not sufficient to achieve optimal efficiency and fidelity for DNA polymerase. Base pair hydrogen bonding contributes at least a third of the energy underlying nucleoside incorporation efficiency and specificity.


Journal of Biological Chemistry | 2008

Base pair hydrogen bonds are essential for proofreading selectivity by the human mitochondrial DNA polymerase

Harold R. Lee; Sandra A. Helquist; Eric T. Kool; Kenneth A. Johnson

We have characterized the role of Watson-Crick hydrogen bonding in the 3′-terminal base pair on the 3′-5′ exonuclease activity of the human mitochondrial DNA polymerase. Nonpolar nucleoside analogs of thymidine (dF) and deoxyadenosine (dQ) were used to eliminate hydrogen bonds while maintaining base pair size and shape. Exonuclease reactions were examined using pre-steady state kinetic methods. The time dependence of removal of natural nucleotides from the primer terminus paired opposite the nonpolar analogs dF and dQ were best fit to a double exponential function. The double exponential kinetics as well as the rates of excision (3–6 s–1 fast phase, 0.16–0.3 s–1 slow phase) are comparable with those observed during mismatch removal of natural nucleotides even when the analog was involved in a sterically correct base pair. Additionally, incorporation of the next correct base beyond a nonpolar analog was slow (0.04–0.22 s–1), so that more than 95% of terminal base pairs were removed rather than extended. The polymerase responds to all 3′-terminal base pairs containing a nonpolar analog as if it were a mismatch regardless of the identity of the paired base, and kinetic partitioning between polymerase and exonuclease sites failed to discriminate between correct and incorrect base pairs. Thus, sterics alone are insufficient, whereas hydrogen bond formation is essential for proper proofreading selectivity by the mitochondrial polymerase. The enzyme may use the alignment and prevention of fraying provided by proper hydrogen bonding and minor groove hydrogen bonding interactions as critical criteria for correct base pair recognition.


Biochemistry | 2006

Varying DNA base-pair size in subangstrom increments: evidence for a loose, not large, active site in low-fidelity Dpo4 polymerase.

Shin Mizukami; Tae Woo Kim; Sandra A. Helquist; Eric T. Kool


Journal of the American Chemical Society | 2003

Probing the requirements for recognition and catalysis in Fpg and MutY with nonpolar adenine isosteres.

Anthony W. Francis; Sandra A. Helquist; Eric T. Kool; Sheila S. David


Journal of Organic Chemistry | 2002

A highly effective nonpolar isostere of deoxyguanosine: synthesis, structure, stacking, and base pairing.

Bryan M. O'Neill; Jessica E. Ratto; Kristi L. Good; Deborah C. Tahmassebi; Sandra A. Helquist; Juan C. Morales; Eric T. Kool


Molecular and Cellular Biology | 2005

Evidence for a Watson-Crick Hydrogen Bonding Requirement in DNA Synthesis by Human DNA Polymerase κ

William T. Wolfle; M. Todd Washington; Eric T. Kool; Thomas E. Spratt; Sandra A. Helquist; Louise Prakash; Satya Prakash


Biochemistry | 2006

Dynamics of nucleotide incorporation: snapshots revealed by 2-aminopurine fluorescence studies.

Chithra Hariharan; Linda B. Bloom; Sandra A. Helquist; Eric T. Kool; Linda J. Reha-Krantz

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Harold R. Lee

University of Texas at Austin

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Kenneth A. Johnson

University of Texas at Austin

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Louise Prakash

University of Texas Medical Branch

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Satya Prakash

University of Texas Medical Branch

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Juan C. Morales

Spanish National Research Council

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Bryan M. O'Neill

San Diego State University

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