Sang Keun Jin

RNA-Seq approach for genetic improvement of meat quality in pig and evolutionary insight into the substrate specificity of animal carbonyl reductases.

Changes in meat quality traits are strongly associated with alterations in postmortem metabolism which depend on genetic variations, especially nonsynonymous single nucleotide variations (nsSNVs) having critical effects on protein structure and function. To selectively identify metabolism-related nsSNVs, next-generation transcriptome sequencing (RNA-Seq) was carried out using RNAs from porcine liver, which contains a diverse range of metabolic enzymes. The multiplex SNV genotyping analysis showed that various metabolism-related genes had different nsSNV alleles. Moreover, many nsSNVs were significantly associated with multiple meat quality traits. Particularly, ch7:g.22112616A>G SNV was identified to create a single amino acid change (Thr/Ala) at the 145th residue of H1.3-like protein, very close to the putative 147th threonine phosphorylation site, suggesting that the nsSNV may affect multiple meat quality traits by affecting the epigenetic regulation of postmortem metabolism-related gene expression. Besides, one nonsynonymous variation, probably generated by gene duplication, led to a stop signal in porcine testicular carbonyl reductase (PTCR), resulting in a C-terminal (E281-A288) deletion. Molecular docking and energy minimization calculations indicated that the binding affinity of wild-type PTCR to 5α-DHT, a C21-steroid, was superior to that of C-terminal-deleted PTCR or human carbonyl reductase, which was very consistent with experimental data, reported previously. Furthermore, P284 was identified as an important residue mediating the specific interaction between PTCR and 5α-DHT, and phylogenetic analysis showed that P284 is an evolutionarily conserved residue among animal carbonyl reductases, which suggests that the C-terminal tails of these reductases may have evolved under evolutionary pressure to increase the substrate specificity for C21-steroids and facilitate metabolic adaptation. Altogether, our RNA-Seq revealed that selective nsSNVs were associated with meat quality traits that could be useful for successful marker-assisted selection in pigs and also represents a useful resource to enhance understanding of protein folding, substrate specificity, and the evolution of enzymes such as carbonyl reductase.

Effect of Hot-Air Dried Tomato Powder on the Quality Properties of Pork Patties during Cold Storage

ABSTRACT The effects of hot air dried tomato powder on the physicochemical and sensory properties of meat patties were studied. The control (C, no addition) and 4 treatments with addition of hot air dried tomato powder (T1, 0.25; T2, 0.50; T3, 0.75; and T4, 1.00%) were prepared and stored for 7 days at 5. The pH values of T4 were significantly lower (p 0.05) than that of control. a* and b* of T4 were the highest (p<0.05) among the all products. Total plate counts (TPC) increased (p<0.05) significantly as the storage period increased. The result of TPC showed the range of 5.48 (T2)~6.98 (C) log CFU/g at the 7 day of storage. Sensory panels evaluated that pork patties containing hot air dried tomato powder had the slightly higher score in overall acceptability. (

The effects of replacement of antibiotics with by-products of oriental medicinal plants on growth performance and meat qualities in fattening pigs

The effect of by-products of oriental medicinal plants (OMP; T1) containing 0.03% herb extracts (T2) or 0.1% aminolevulinic acid (T3) on the production performance of swine during the finishing period and on its meat quality were investigated. No significant differences were found in the weight gain, feed intake and feed conversion rate among the tested groups (P > 0.05). But the treated group showed higher (P < 0.05) moisture and ash and lower protein than the control group. The T3 group showed a lower meat cholesterol content (38.42 mg/100 g) compared to the other groups (P < 0.05). The vitamin E content of the muscle in the treated groups was higher compared to the control group. No antibiotic content was detected in all treated and control samples. The values of the volatile basic nitrogen (VBN) and thiobarbituric acid reactive substance (TBARS) of the treated groups were significantly lower (P < 0.01) than the control group. The treated groups had significantly better (P < 0.05) sensory-test scores for color, flavor, off-flavor and total acceptability compared to the control group.

Changes of Quality Properties of Pork Loaves with Chemical-free Sweet Persimmon Powder during Chilled Storage at 5

Four different pork loaves were made with two levels, HP/FP-3% and HP/FP-6%, containing 3.0 and 6.0% HP (hot air dried sweet persimmon powder) / FP (freezer dried sweet persimmon powder), respectively. HP and FP (3% and 6%) were added into ground pork loin separately. A control without persimmon powder were used for comparison. Patties were cooked to 74 in electric oven, after cooling, the aerobic packaged meat loaves were stored at 5 . The moisture and crude ash were higher in the FP-6%, and the crude protein and crude fat were no different among the samples. The pH in control sample was significantly higher (p<0.05) than those of treatment group, however no significant difference was found among the 4 treatments. The TBARS (thiobarbituric reactive substance) values of all samples showed a tendency of increasing value along the storage days. The TBARS and a* value increased (p<0.05) as the addition ratio of HP/FP increased, respectively. With regard to microorganisms, the number of total aerobic bacteria were lower than 3.11 log10 CFU/g. In sensory evaluation, meat loaves containing persimmon powder resulted in a high overall acceptability, although they were not significantly different in overall

Comparative proteomic analysis of the effects of dietary mugwort ( Artemisia iwayomogi Kitamura ) on the pig Longissimus dorsi muscle

Proteomic analysis by 2-dimensional electrophoresis (2-DE) of the longissimus dorsi muscle in finishing pigs (LY × D) grown on a diet supplemented with mugwort powder found roughly 300 spots on a polyacrylamide gel image. Among them, the expression levels of 12 spots were higher than those of the control group. From the results of peptide mass fingerprinting (PMF) analysis, nine proteins were characterized: myosin light chain 2V, elF-5A, myosin light chain 1, F1-ATPase chain D, peroxiredoxin-2, Kelch-related protein 1, SLA-7, glycine amidinotransferase, and Tpi 1. The other three spots with increased expression were identified by chemically assisted fragmentation-matrix-assisted laser desorption/ionization (CAF-MALDI) sequencing as serum albumin precursor and two different types of myoglobin. Taken together, these results indicate that mugwort powder has the potential to improve the quality of pork meat. Key words : 2-DE, longissimus dorsi muscle, meat quality, mugwort, pig, proteome.