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Dive into the research topics where Sang Su Shin is active.

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Featured researches published by Sang Su Shin.


The FASEB Journal | 2008

Generation of transgenic quail through germ cell-mediated germline transmission

Sang Su Shin; Tae Min Kim; Sunyoung Kim; Tae Wan Kim; Hee Won Seo; Seul Ki Lee; Se Chang Kwon; Gwan Sun Lee; Heebal Kim; Jeong Mook Lim; Jae Yong Han

Here, we describe the production of transgenic quail via a germline transmission system using postmigratory gonadal primordial germ cells (gPGCs). gPGCs retrieved from the embryonic gonads of 5‐day‐old birds were transduced with a lentiviral vector and subsequently transferred into recipient embryos. Testcross and genetic analyses revealed that among three germline chimeric G0 quail, one male produced transgenic offspring;of 310 hatchlings from the transgenic germline chimera, 24 were identified as donor‐derived offspring, and 6 were transgenic (6/310, 1.9%). Conventional transgenesis using stage × blastodermal embryos was also conducted, but the efficiency of transgenesis was similar between the two systems (<1.6 vs. 1.9% for the conventional and gPGC‐mediated systems, respectively). However, substantial advantages can be gained from gPGC‐mediated method in that it enables an induced germline modification, whereas direct retroviral transfer to stage X embryos causes mosaic integration. The use of gonadal PGCs for transgenesis may lead to the production of bioreactors.—Shin, S. S., Kim, T. M., Kim, S. Y., Kim, T. W., Seo, H. W., Lee, S. K., Kwon, S. C., Lee, G. S., Kim, H., Lim, J. M., Han, J. Y. Generation of transgenic quail through germ cell‐mediated germline transmission. FASEB J. 22, 2435–2444 (2008)


Biology of Reproduction | 2006

A Testis-Mediated Germline Chimera Production Based on Transfer of Chicken Testicular Cells Directly into Heterologous Testes

Young Mok Lee; Jin Gyoung Jung; Jin Nam Kim; Tae Sub Park; Tae Min Kim; Sang Su Shin; Dae Kyung Kang; Jeong Mook Lim; Jae Yong Han

Abstract In this study, we proposed a testis-mediated germline chimera production system based on the transplantation of testicular cells directly into heterologous testes. The testicular cells of juvenile (4-wk-old) or adult (24-wk-old) Korean Ogol chickens with a recessive pigmentation inhibitory gene, with or without prior culture, were injected (2 × 107 cells/head) into the seminiferous tubules of juvenile or adult recipients with White Leghorn with a dominant pigmentation inhibitory gene in a 2 × 2 factorial arrangement. The localization of transplanted cells into the inner space of the seminiferous tubules was confirmed within 24 h after injection. Subsequent testcross analyses showed that 7.8% (5/64) of the recipients had chimeric status in their testes. The periods of time from transfer to hatching of the first progeny with black feathers were 38 and 45 days for adult cells transplanted into an adult recipient, 188 days for adult cells into a juvenile recipient, and 137 days for juvenile cells into a juvenile recipient. Culture of the testicular cells derived both colony-forming and monolayer-forming cells. The colony-forming cells were stained positively for periodic acid Schiff solution, and further reacted with anti-SSEA-1, anti-SSEA-3, and anti-SSEA-4 antibodies both before and after culture for 15 days. In conclusion, it may be possible to develop the testis-mediated germline chimera production technique, which extends the feasibility of genetic manipulations in avian species.


Biology of Reproduction | 2010

Production of Biofunctional Recombinant Human Interleukin 1 Receptor Antagonist (rhIL1RN) from Transgenic Quail Egg White

Se Chang Kwon; Jin Won Choi; Hyun Jun Jang; Sang Su Shin; Seul Ki Lee; Tae Sub Park; In Young Choi; Gwan Sun Lee; Gwonhwa Song; Jae Yong Han

Oviduct-specific expression of heterologous recombinant proteins in transgenic birds is a promising technology for the large-scale production of therapeutic proteins in eggs. We describe the production of recombinant human interleukin 1 receptor antagonist (rhIL1RN) in the eggs of transgenic quails. To drive tissue-specific expression of rhIL1RN, a 1.35-kb fragment of the chicken ovalbumin promoter, which contains both the steroid-dependent regulatory element and the negative regulatory element, was used. A transgenic quail was generated by microinjection of a concentrated stock of lentivirus into stage X blastodermal cells. A single copy of the transgene was integrated into the seventh intron of the gene for conserved oligomeric golgi complex protein 5 (COG5) on chromosome 1. As expected, rhIL1RN expression was restricted to oviductal tissue, and the amount of protein deposited in the eggs of homozygous transgenic quails ranged from 88.7 to 233.8 ng/ml. Transgene expression was conserved from the G1 generation to the G4 generation, and there was no evidence of transgene silencing. In a bioassay using the EL4.NOB-1/CTLL-2 coculture system, no significant difference was observed between the egg-produced rhIL1RN and a commercially available rhIL1RN (anakinra).


Molecular Reproduction and Development | 2006

Establishment of an in vitro culture system for chicken preblastodermal cells

Hyun Park; Tae Sub Park; Tae Min Kim; Jin Nam Kim; Sang Su Shin; Jeong Mook Lim; Jae Yong Han


Physiological Genomics | 2007

MPSS profiling of embryonic gonad and primordial germ cells in chicken

Heebal Kim; Tae Sub Park; Woon Kyu Lee; S. J. Moon; Jin Nam Kim; Ji Hye Shin; Jin Gyoung Jung; Seon Duk Lee; Sang Hyun Park; Kyung Je Park; Mi A Kim; Sang Su Shin; Tae Min Kim; Jungrye Nam; Yeonkyung Kang; Jeong Mook Lim; Jae Yong Han


한국가금학회 정기총회 및 학술발표회 | 2004

Peroxiredoxin(PRX) gene family characterization in aves

Sang Su Shin; Tae Min Kim; Ji Hye Shin; Tae Sub Park; Jin-Kyoo Kim; Heebal Kim; Jae Yong Han


Biology of Reproduction | 2008

Transgene Mapping and Expression Analysis in Transgenic Birds Produced by Lentiviral Transduction of Gonadal Primordial Germ Cells.

Tae Min Kim; Sang Su Shin; Sunyoung Kim; Tae Wan Kim; Hee Won Seo; Seul Ki Lee; Heebal Kim; Jeong Mook Lim; Jae Yong Han


한국가금학회 정기총회 및 학술발표회 | 2007

Production of Transgenic Aves

Tae Min Kim; Sang Su Shin; Seul Ki Lee; Jin Nam Kim; Jeong Mook Lim; Jae Yong Han


한국가금학회 정기총회 및 학술발표회 | 2006

Induction and Gene Manipulation of Chicken Oviduct Epithelial Cells

Hee Won Seo; Sun Young Kim; Sang Su Shin; Tae Min Kim; Young Mok Lee; Bo Ram Lee; Tae Wan Kim; Jeong Mook Lim; Jae Yong Han


한국가금학회 정기총회 및 학술발표회 | 2003

Development of Expressed Sequence Tags(ESTs) from Korean Native Chicken cDNA Libraries

Sang Su Shin; Ki Duk Song; Jee Hye Shin; Sun Duck Lee; Young Mok Lee; Jin Kyu Kim; Jae Yong Han

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Jae Yong Han

Seoul National University

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Tae Min Kim

Seoul National University Hospital

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Jeong Mook Lim

Seoul National University

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Tae Sub Park

Seoul National University

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Jin Nam Kim

Biotechnology Institute

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Heebal Kim

Seoul National University

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Seul Ki Lee

Biotechnology Institute

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Hee Won Seo

Seoul National University

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Hyun Park

Seoul National University

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Tae Wan Kim

Seoul National University

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