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Dive into the research topics where Sarah Bertelsen is active.

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Featured researches published by Sarah Bertelsen.


American Journal of Psychiatry | 2008

Variants in Nicotinic Receptors and Risk for Nicotine Dependence

Laura J. Bierut; Jerry A. Stitzel; Jen C. Wang; Anthony L. Hinrichs; Richard A. Grucza; Xiaoling Xuei; Nancy L. Saccone; Scott F. Saccone; Sarah Bertelsen; Louis Fox; William J. Horton; Naomi Breslau; John Budde; C. Robert Cloninger; Danielle M. Dick; Tatiana Foroud; Dorothy K. Hatsukami; Victor Hesselbrock; Eric O. Johnson; John Kramer; Samuel Kuperman; Pamela A. F. Madden; Kevin Mayo; John I. Nurnberger; Ovide F. Pomerleau; Bernice Porjesz; Oliver Reyes; Marc A. Schuckit; Gary E. Swan; Jay A. Tischfield

OBJECTIVE A recent study provisionally identified numerous genetic variants as risk factors for the transition from smoking to the development of nicotine dependence, including an amino acid change in the alpha5 nicotinic cholinergic receptor (CHRNA5). The purpose of this study was to replicate these findings in an independent data set and more thoroughly investigate the role of genetic variation in the cluster of physically linked nicotinic receptors, CHRNA5-CHRNA3-CHRNB4, and the risk of smoking. METHOD Individuals from 219 European American families (N=2,284) were genotyped across this gene cluster to test the genetic association with smoking. The frequency of the amino acid variant (rs16969968) was studied in 995 individuals from diverse ethnic populations. In vitro studies were performed to directly test whether the amino acid variant in the CHRNA5 influences receptor function. RESULTS A genetic variant marking an amino acid change showed association with the smoking phenotype (p=0.007). This variant is within a highly conserved region across nonhuman species, but its frequency varied across human populations (0% in African populations to 37% in European populations). Furthermore, functional studies demonstrated that the risk allele decreased response to a nicotine agonist. A second independent finding was seen at rs578776 (p=0.003), and the functional significance of this association remains unknown. CONCLUSIONS This study confirms that at least two independent variants in this nicotinic receptor gene cluster contribute to the development of habitual smoking in some populations, and it underscores the importance of multiple genetic variants contributing to the development of common diseases in various populations.


Proceedings of the National Academy of Sciences of the United States of America | 2010

A genome-wide association study of alcohol dependence

Laura Jean Bierut; Arpana Agrawal; Kathleen K. Bucholz; Kimberly F. Doheny; Cathy C. Laurie; Elizabeth W. Pugh; Sherri L. Fisher; Louis Fox; William B. Howells; Sarah Bertelsen; Anthony L. Hinrichs; Laura Almasy; Naomi Breslau; Robert Culverhouse; Danielle M. Dick; Howard J. Edenberg; Tatiana Foroud; Richard A. Grucza; Dorothy K. Hatsukami; Victor Hesselbrock; Eric O. Johnson; John Kramer; Robert F. Krueger; Samuel Kuperman; Michael T. Lynskey; Karl Mann; Rosalind J. Neuman; Markus M. Nöthen; John I. Nurnberger; Bernice Porjesz

Excessive alcohol consumption is one of the leading causes of preventable death in the United States. Approximately 14% of those who use alcohol meet criteria during their lifetime for alcohol dependence, which is characterized by tolerance, withdrawal, inability to stop drinking, and continued drinking despite serious psychological or physiological problems. We explored genetic influences on alcohol dependence among 1,897 European-American and African-American subjects with alcohol dependence compared with 1,932 unrelated, alcohol-exposed, nondependent controls. Constitutional DNA of each subject was genotyped using the Illumina 1M beadchip. Fifteen SNPs yielded P < 10−5, but in two independent replication series, no SNP passed a replication threshold of P < 0.05. Candidate gene GABRA2, which encodes the GABA receptor α2 subunit, was evaluated independently. Five SNPs at GABRA2 yielded nominal (uncorrected) P < 0.05, with odds ratios between 1.11 and 1.16. Further dissection of the alcoholism phenotype, to disentangle the influence of comorbid substance-use disorders, will be a next step in identifying genetic variants associated with alcohol dependence.


Nature | 2014

Rare coding variants in the phospholipase D3 gene confer risk for Alzheimer's disease

Carlos Cruchaga; Celeste M. Karch; Sheng Chih Jin; Bruno A. Benitez; Yefei Cai; Rita Guerreiro; Oscar Harari; Joanne Norton; John Budde; Sarah Bertelsen; Amanda T. Jeng; Breanna Cooper; Tara Skorupa; David Carrell; Denise Levitch; Simon Hsu; Jiyoon Choi; Mina Ryten; John Hardy; Daniah Trabzuni; Michael E. Weale; Adaikalavan Ramasamy; Colin Smith; Celeste Sassi; Jose Bras; J. Raphael Gibbs; Dena Hernandez; Michelle K. Lupton; John Powell; Paola Forabosco

Genome-wide association studies (GWAS) have identified several risk variants for late-onset Alzheimers disease (LOAD). These common variants have replicable but small effects on LOAD risk and generally do not have obvious functional effects. Low-frequency coding variants, not detected by GWAS, are predicted to include functional variants with larger effects on risk. To identify low-frequency coding variants with large effects on LOAD risk, we carried out whole-exome sequencing (WES) in 14 large LOAD families and follow-up analyses of the candidate variants in several large LOAD case–control data sets. A rare variant in PLD3 (phospholipase D3; Val232Met) segregated with disease status in two independent families and doubled risk for Alzheimer’s disease in seven independent case–control series with a total of more than 11,000 cases and controls of European descent. Gene-based burden analyses in 4,387 cases and controls of European descent and 302 African American cases and controls, with complete sequence data for PLD3, reveal that several variants in this gene increase risk for Alzheimer’s disease in both populations. PLD3 is highly expressed in brain regions that are vulnerable to Alzheimer’s disease pathology, including hippocampus and cortex, and is expressed at significantly lower levels in neurons from Alzheimer’s disease brains compared to control brains. Overexpression of PLD3 leads to a significant decrease in intracellular amyloid-β precursor protein (APP) and extracellular Aβ42 and Aβ40 (the 42- and 40-residue isoforms of the amyloid-β peptide), and knockdown of PLD3 leads to a significant increase in extracellular Aβ42 and Aβ40. Together, our genetic and functional data indicate that carriers of PLD3 coding variants have a twofold increased risk for LOAD and that PLD3 influences APP processing. This study provides an example of how densely affected families may help to identify rare variants with large effects on risk for disease or other complex traits.


Molecular Psychiatry | 2009

Genetic variation in the CHRNA5 gene affects mRNA levels and is associated with risk for alcohol dependence.

Jen-Chyong Wang; Richard A. Grucza; Carlos Cruchaga; Anthony L. Hinrichs; Sarah Bertelsen; John Budde; Louis Fox; E Goldstein; Oliver Reyes; Nancy L. Saccone; Scott F. Saccone; Xiaoling Xuei; K. K. Bucholz; Samuel Kuperman; John I. Nurnberger; John P. Rice; M. Schuckit; Jay A. Tischfield; Victor Hesselbrock; Bernice Porjesz; Howard J. Edenberg; Laura J. Bierut; Alison Goate

Alcohol dependence frequently co-occurs with cigarette smoking, another common addictive behavior. Evidence from genetic studies demonstrates that alcohol dependence and smoking cluster in families and have shared genetic vulnerability. Recently a candidate gene study in nicotine dependent cases and nondependent smoking controls reported strong associations between a missense mutation (rs16969968) in exon 5 of the CHRNA5 gene and a variant in the 3′-UTR of the CHRNA3 gene and nicotine dependence. In this study we performed a comprehensive association analysis of the CHRNA5–CHRNA3–CHRNB4 gene cluster in the Collaborative Study on the Genetics of Alcoholism (COGA) families to investigate the role of genetic variants in risk for alcohol dependence. Using the family-based association test, we observed that a different group of polymorphisms, spanning CHRNA5-CHRNA3, demonstrate association with alcohol dependence defined by Diagnostic and Statistical Manual of Mental Disorders, 4th edn (DSM-IV) criteria. Using logistic regression we replicated this finding in an independent case-control series from the family study of cocaine dependence. These variants show low linkage disequilibrium with the SNPs previously reported to be associated with nicotine dependence and therefore represent an independent observation. Functional studies in human brain reveal that the variants associated with alcohol dependence are also associated with altered steady-state levels of CHRNA5 mRNA.


Neuron | 2013

GWAS of Cerebrospinal Fluid Tau Levels Identifies Risk Variants for Alzheimer’s Disease

Carlos Cruchaga; John Kauwe; Oscar Harari; Sheng Chih Jin; Yefei Cai; Celeste M. Karch; Bruno A. Benitez; Amanda T. Jeng; Tara Skorupa; David Carrell; Sarah Bertelsen; Matthew Bailey; David McKean; Joshua M. Shulman; Philip L. De Jager; Lori B. Chibnik; David A. Bennett; Steve E. Arnold; Denise Harold; Rebecca Sims; Amy Gerrish; Julie Williams; Vivianna M. Van Deerlin; Virginia M.-Y. Lee; Leslie M. Shaw; John Q. Trojanowski; Jonathan L. Haines; Richard Mayeux; Margaret A. Pericak-Vance; Lindsay A. Farrer

Cerebrospinal fluid (CSF) tau, tau phosphorylated at threonine 181 (ptau), and Aβ₄₂ are established biomarkers for Alzheimers disease (AD) and have been used as quantitative traits for genetic analyses. We performed the largest genome-wide association study for cerebrospinal fluid (CSF) tau/ptau levels published to date (n = 1,269), identifying three genome-wide significant loci for CSF tau and ptau: rs9877502 (p = 4.89 × 10⁻⁹ for tau) located at 3q28 between GEMC1 and OSTN, rs514716 (p = 1.07 × 10⁻⁸ and p = 3.22 × 10⁻⁹ for tau and ptau, respectively), located at 9p24.2 within GLIS3 and rs6922617 (p = 3.58 × 10⁻⁸ for CSF ptau) at 6p21.1 within the TREM gene cluster, a region recently reported to harbor rare variants that increase AD risk. In independent data sets, rs9877502 showed a strong association with risk for AD, tangle pathology, and global cognitive decline (p = 2.67 × 10⁻⁴, 0.039, 4.86 × 10⁻⁵, respectively) illustrating how this endophenotype-based approach can be used to identify new AD risk loci.


Human Molecular Genetics | 2009

Risk for nicotine dependence and lung cancer is conferred by mRNA expression levels and amino acid change in CHRNA5

Jen C. Wang; Carlos Cruchaga; Nancy L. Saccone; Sarah Bertelsen; Pengyuan Liu; John Budde; Weimin Duan; Louis Fox; Richard A. Grucza; Jason Kern; Kevin H. Mayo; Oliver Reyes; John R. Rice; Scott F. Saccone; Noah Spiegel; Joseph H. Steinbach; Jerry A. Stitzel; Marshall W. Anderson; Ming You; Victoria L. Stevens; Laura J. Bierut; Alison Goate

Nicotine dependence risk and lung cancer risk are associated with variants in a region of chromosome 15 encompassing genes encoding the nicotinic receptor subunits CHRNA5, CHRNA3 and CHRNB4. To identify potential biological mechanisms that underlie this risk, we tested for cis-acting eQTLs for CHRNA5, CHRNA3 and CHRNB4 in human brain. Using gene expression and disease association studies, we provide evidence that both nicotine-dependence risk and lung cancer risk are influenced by functional variation in CHRNA5. We demonstrated that the risk allele of rs16969968 primarily occurs on the low mRNA expression allele of CHRNA5. The non-risk allele at rs16969968 occurs on both high and low expression alleles tagged by rs588765 within CHRNA5. When the non-risk allele occurs on the background of low mRNA expression of CHRNA5, the risk for nicotine dependence and lung cancer is significantly lower compared to those with the higher mRNA expression. Together, these variants identify three levels of risk associated with CHRNA5. We conclude that there are at least two distinct mechanisms conferring risk for nicotine dependence and lung cancer: altered receptor function caused by a D398N amino acid variant in CHRNA5 (rs16969968) and variability in CHRNA5 mRNA expression.


Biological Psychiatry | 2008

A Risk Allele for Nicotine Dependence in CHRNA5 Is a Protective Allele for Cocaine Dependence

Richard A. Grucza; Jen C. Wang; Jerry A. Stitzel; Anthony L. Hinrichs; Scott F. Saccone; Nancy L. Saccone; Kathleen K. Bucholz; C. Robert Cloninger; Rosalind J. Neuman; John Budde; Louis Fox; Sarah Bertelsen; John Kramer; Victor Hesselbrock; Jay A. Tischfield; John I. Nurnberger; Laura Almasy; Bernice Porjesz; Samuel Kuperman; Marc A. Schuckit; Howard J. Edenberg; John P. Rice; Alison Goate; Laura J. Bierut

BACKGROUND A nonsynonymous coding polymorphism, rs16969968, of the CHRNA5 gene that encodes the alpha-5 subunit of the nicotinic acetylcholine receptor (nAChR) has been found to be associated with nicotine dependence. The goal of this study was to examine the association of this variant with cocaine dependence. METHODS Genetic association analysis was performed in two independent samples of unrelated case and control subjects: 1) 504 European Americans participating in the Family Study on Cocaine Dependence (FSCD) and 2) 814 European Americans participating in the Collaborative Study on the Genetics of Alcoholism (COGA). RESULTS In the FSCD, there was a significant association between the CHRNA5 variant and cocaine dependence (odds ratio = .67 per allele, p = .0045, assuming an additive genetic model), but in the reverse direction compared with that previously observed for nicotine dependence. In multivariate analyses that controlled for the effects of nicotine dependence, both the protective effect for cocaine dependence and the previously documented risk effect for nicotine dependence were statistically significant. The protective effect for cocaine dependence was replicated in the COGA sample. In COGA, effect sizes for habitual smoking, a proxy phenotype for nicotine dependence, were consistent with those observed in FSCD. CONCLUSIONS The minor (A) allele of rs16969968, relative to the major G allele, appears to be both a risk factor for nicotine dependence and a protective factor for cocaine dependence. The biological plausibility of such a bidirectional association stems from the involvement of nAChRs with both excitatory and inhibitory modulation of dopamine-mediated reward pathways.


Molecular Psychiatry | 2012

ADH1B is associated with alcohol dependence and alcohol consumption in populations of European and African ancestry

Laura J. Bierut; Alison Goate; Naomi Breslau; Eric O. Johnson; Sarah Bertelsen; Louis Fox; Arpana Agrawal; Kathleen K. Bucholz; Richard A. Grucza; Victor Hesselbrock; John Kramer; Samuel Kuperman; John I. Nurnberger; Bernice Porjesz; Nancy L. Saccone; Marc A. Schuckit; Jay A. Tischfield; Jen C. Wang; Tatiana Foroud; John P. Rice; Howard J. Edenberg

A coding variant in alcohol dehydrogenase 1B (ADH1B) (rs1229984) that leads to the replacement of Arg48 with His48 is common in Asian populations and reduces their risk for alcoholism, but because of very low allele frequencies the effects in European or African populations have been difficult to detect. We genotyped and analyzed this variant in three large European and African-American case–control studies in which alcohol dependence was defined by the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition (DSM-IV) criteria, and demonstrated a strong protective effect of the His48 variant (odds ratio (OR) 0.34, 95% confidence interval (CI) 0.24, 0.48) on alcohol dependence, with genome-wide significance (6.6 × 10–10). The hypothesized mechanism of action involves an increased aversive reaction to alcohol; in keeping with this hypothesis, the same allele is strongly associated with a lower maximum number of drinks in a 24-hour period (lifetime), with P=3 × 10–13. We also tested the effects of this allele on the development of alcoholism in adolescents and young adults, and demonstrated a significantly protective effect. This variant has the strongest effect on risk for alcohol dependence compared with any other tested variant in European populations.


American Journal of Human Genetics | 2006

Functional variant in a bitter-taste receptor (hTAS2R16) influences risk of alcohol dependence

Anthony L. Hinrichs; Jen C. Wang; Bernd Bufe; Jennifer M. Kwon; John Budde; Rebecca Allen; Sarah Bertelsen; Whitney Evans; Danielle M. Dick; John P. Rice; Tatiana Foroud; John I. Nurnberger; Jay A. Tischfield; Samuel Kuperman; Raymond R. Crowe; Victor Hesselbrock; Marc A. Schuckit; Laura Almasy; Bernice Porjesz; Howard J. Edenberg; Henri Begleiter; Wolfgang Meyerhof; Laura J. Bierut; Alison Goate

A coding single-nucleotide polymorphism (cSNP), K172N, in hTAS2R16, a gene encoding a taste receptor for bitter beta -glucopyranosides, shows significant association with alcohol dependence (P = .00018). This gene is located on chromosome 7q in a region reported elsewhere to exhibit linkage with alcohol dependence. The SNP is located in the putative ligand-binding domain and is associated with an increased sensitivity to many bitter beta -glucopyranosides in the presence of the N172 allele. Individuals with the ancestral allele K172 are at increased risk of alcohol dependence, regardless of ethnicity. However, this risk allele is uncommon in European Americans (minor-allele frequency [MAF] 0.6%), whereas 45% of African Americans carry the allele (MAF 26%), which makes it a much more significant risk factor in the African American population.


PLOS Genetics | 2010

SNPs Associated with Cerebrospinal Fluid Phospho-Tau Levels Influence Rate of Decline in Alzheimer's Disease

Carlos Cruchaga; John Kauwe; Kevin Mayo; Noah Spiegel; Sarah Bertelsen; Petra Nowotny; Aarti R. Shah; Richard Abraham; Paul Hollingworth; Denise Harold; Michael John Owen; Julie Williams; Simon Lovestone; Elaine R. Peskind; Ge Li; James B. Leverenz; Douglas Galasko; John C. Morris; Anne M. Fagan; David M. Holtzman; Alison Goate

Alzheimers Disease (AD) is a complex and multifactorial disease. While large genome-wide association studies have had some success in identifying novel genetic risk factors for AD, case-control studies are less likely to uncover genetic factors that influence progression of disease. An alternative approach to identifying genetic risk for AD is the use of quantitative traits or endophenotypes. The use of endophenotypes has proven to be an effective strategy, implicating genetic risk factors in several diseases, including anemia, osteoporosis and heart disease. In this study we identify a genetic factor associated with the rate of decline in AD patients and present a methodology for identification of other such factors. We have used an established biomarker for AD, cerebrospinal fluid (CSF) tau phosphorylated at threonine 181 (ptau181) levels as an endophenotype for AD, identifying a SNP, rs1868402, in the gene encoding the regulatory sub-unit of protein phosphatase B, associated with CSF ptau181 levels in two independent CSF series . We show no association of rs1868402 with risk for AD or age at onset, but detected a very significant association with rate of progression of disease that is consistent in two independent series . Our analyses suggest that genetic variants associated with CSF ptau181 levels may have a greater impact on rate of progression, while genetic variants such as APOE4, that are associated with CSF Aβ42 levels influence risk and onset but not the rate of progression. Our results also suggest that drugs that inhibit or decrease tau phosphorylation may slow cognitive decline in individuals with very mild dementia or delay the appearance of memory problems in elderly individuals with low CSF Aβ42 levels. Finally, we believe genome-wide association studies of CSF tau/ptau181 levels should identify novel genetic variants which will likely influence rate of progression of AD.

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Alison Goate

Icahn School of Medicine at Mount Sinai

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Anthony L. Hinrichs

Washington University in St. Louis

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John Kauwe

Brigham Young University

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Carlos Cruchaga

Washington University in St. Louis

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David M. Holtzman

Washington University in St. Louis

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John C. Morris

Washington University in St. Louis

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Laura J. Bierut

Washington University in St. Louis

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Anne M. Fagan

Washington University in St. Louis

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Victor Hesselbrock

Virginia Commonwealth University

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John Budde

Washington University in St. Louis

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