Sarah J. Beal

Salivary Cytokines in Healthy Adolescent Girls: Intercorrelations, Stability, and Associations with Serum Cytokines, Age and Pubertal Stage

Theoretically, the measurement of cytokines in saliva may have utility for studies of brain, behavior, and immunity in youth. Cytokines in saliva and serum were analyzed across three annual assessments in healthy adolescent girls (N = 114, 11-17 years at enrollment). Samples were assayed for GM-CSF, IFNγ, IL-1β, IL-2, IL-6, IL-8, IL-10, IL-12p70, TNFα, adiponectin, and cotinine. Results revealed: (1) cytokine levels, except IFNγ and IL-10, were detectable in saliva, and salivary levels, except IL-8 and IL-1β, were lower than serum levels; (2) salivary cytokine levels were lower in older girls and positively associated with adiponectin; (3) compared to serum levels, the correlations between salivary cytokines were higher, but salivary cytokines were less stable across years; and (4) except for IL-1β, there were no significant serum-saliva associations. Variation in basal salivary cytokine levels in healthy adolescent girls reflect compartmentalized activity of the oral mucosal immune system, rather than systemic cytokine activity.

Longitudinal Impact of Substance Use and Depressive Symptoms on Bone Accrual Among Girls Aged 11–19 Years

PURPOSE Osteoporosis is primarily evident in postmenopausal women, but its roots are traceable to periods of growth, including during adolescence. Depression, anxiety, and smoking are associated with lower bone mineral density (BMD) in adults. These associations have not been studied longitudinally across adolescence, when more than 50% of bone accrual occurs. METHODS To determine the impact of depressive and anxiety symptoms, smoking, and alcohol use on bone accrual in girls 11-19 years, 262 healthy girls were enrolled in age cohorts of 11, 13, 15, and 17 years. Using a cross-sequential design, girls were seen for three annual visits. Outcome measures included total body bone mineral content and BMD of the total hip and lumbar spine using dual energy x-ray absorptiometry. Depressive and anxiety symptoms and smoking and alcohol use were by self-report. RESULTS Higher-frequency smoking was associated with a lower rate of lumbar spine and total hip BMD accrual from ages 13-19. Higher depressive symptoms were associated with lower lumbar spine BMD across 11-19 years of age. There was no effect of depressive symptoms on total body bone mineral content, and there was no effect of alcohol intake on any bone outcome. CONCLUSION Adolescent smokers are at higher risk for less than optimal bone accrual. Even in the absence of diagnosable depression, depressive symptoms may influence adolescent bone accrual. These findings have import for prevention of later osteoporosis and fractures.

Children's participation in foster care hearings

PURPOSE Empirical research regarding potential risks and benefits of childrens participation in the legal system generally, and in the child dependency legal system in particular, is sparse and mostly characterized by small studies without comparison groups. The current study was designed to address the following questions regarding childrens participation in dependency court hearings: (1) Is attending court harmful to children? (2) Is attending court beneficial to children? (3) Is judicial behavior with the child in the courtroom related to potential harms or benefits? and (4) Are there age differences in childrens reactions? METHOD The authors measured childrens reactions to attending dependency review hearings (n=43) and compared them to a sample of children who did not attend their hearings (n=50). One to 2 weeks following review hearings, both groups of children were interviewed about their reactions to the court process. RESULTS Children who attended their hearings reported more positive feelings about the dependency process (e.g., trust in judge, perceived fairness, and more comfort with their guardians ad litem and caseworkers). For children who attended, there was no evidence of high distress immediately preceding or following their hearings. Court observations revealed that more active engagement by judges was related to positive responses from the children. Most children, including both children who attended hearings and those who did not, believed that all children should be able to attend their hearings. CONCLUSION Overall, the findings suggest that policies encouraging childrens attendance at dependency hearings are viewed positively by and not harmful to children.

An Introduction to Propensity Scores: What, When, and How

The use of propensity scores as a method to promote causality in studies that cannot use random assignment has increased dramatically since its original publication in 1983. While the utility of these approaches is important, the concepts underlying their use are complex. The purpose of this article is to provide a basic tutorial for conducting analyses using propensity scores and what researchers should be aware of in reading papers that choose propensity scores as a method, as well as in conducting their own research. In addition to the explanations given, examples are presented, based on actual studies, which illustrate the use of propensity scores for regression adjustment, stratification, and matching. The syntax, datasets, and output used for these examples are available on http://jea.sagepub.com/content/early/recent for readers to download and follow.

Foster Caregivers’ Perspectives on the Medical Challenges of Children Placed in Their Care Implications for Pediatricians Caring for Children in Foster Care

Objective. To investigate, using qualitative methodology, foster caregivers’ perspectives related to the medical needs of children placed in their care. Study design. Fifteen foster caregivers were individually interviewed using a semistructured open-ended question guide. Data were coded, and the analysis was conducted in an inductive manner, allowing themes to emerge from the interviews. Results. The following 4 themes were identified: (1) the fragmented histories provided to foster caregivers and difficulty in obtaining information; (2) the unique medical complications that children in foster care experience; (3) the difference between “doing what is expected” and becoming a proactive foster caregiver; and (4) the support needs of foster caregivers. Conclusions. Foster caregivers receive insufficient information despite the evidence that these children are likely to have complex needs. It is, therefore, necessary for the pediatrician to recognize existing medical problems, identify new medical problems, educate foster caregivers, and communicate with the multidisciplinary team.

Characterizing the Longitudinal Relations between Depressive and Menstrual Symptoms in Adolescent Girls

Objective This study examined the association between depressive and menstrual symptoms in adolescent girls in a 3-year longitudinal study. It was hypothesized that menstrual symptoms would increase in early adolescence and decrease in later adolescence, that girls with greater depressive symptoms would report greater menstrual symptoms, and that effects would persist after adjusting for general somatic complaints. Methods A community sample of girls (n = 262) enrolled in an observational study by age cohort (11, 13, 15, 17 years) completed three annual visits. At each time point, girls completed the Menstrual Symptom Questionnaire, Children’s Depression Inventory, and the Youth Self Report to assess general somatic complaints. Results Menstrual symptoms increased significantly across adolescence (p = .006) and began to plateau in later adolescence (p = .020). Depressive symptoms at study entry were significantly associated with menstrual symptoms (p < .001). When general somatic complaints were included in the models, the effect of depressive symptoms on menstrual symptoms remained significant for the sum score (p = .015) and the menstrual somatic symptoms subscale (p = .001). After adjusting for somatic complaints, initial report of depressive symptoms predicted change in menstrual symptoms only for girls with the lowest menstrual symptoms sum score (p = .025). Initial report of somatic complaints predicted change in menstrual symptoms (p = .020). Conclusions Girls with higher depressive symptoms and higher somatic complaints are at greater risk for experiencing menstrual symptoms and increasing symptoms across adolescence, with a heightened vulnerability for girls with lower baseline menstrual symptoms.

Children in nonparental care: health and social risks

Approximately 2.3 million children in the United States live separately from both parents; 70–90% of those children live with a relative. Compared with children living with one or both parents, children in nonparental care are in poorer health, are at heightened risk for experiencing disruptions and instability in caregiving, and are vulnerable to other social antecedents of child health (e.g., neglect, poverty, maltreatment). Given the significant impact of adversity in childhood on health across the lifespan, which is increased among children in nonparental care, it is informative to consider the health risks of children living in nonparental care specifically. Research examining the contributions of poverty, instability, child maltreatment, and living in nonparental care, including meta-analyses of existing studies, are warranted. Longitudinal studies describing pathways into and out of nonparental care and the course of health throughout those experiences are also needed. Despite these identified gaps, there is sufficient evidence to indicate that attention to household structure is not only relevant but also essential for the clinical care of children and may aid in identifying youth at risk for developing poor health across the lifespan.

Motivation in the age of genomics: why genetic findings of disease susceptibility might not motivate behavior change

There is a growing consensus that results generated through multiplex genetic tests, even those produced as a part of research, should be reported to providers and patients when they are considered “actionable,” that is, when they could be used to inform some potentially beneficial clinical action. However, there remains controversy over the precise criterion that should be used in identifying when a result meets this standard. In this paper, we seek to refine the concept of “actionability” by exploring one proposed use for genetic test results. We argue that genetic test results indicating that a patient is at risk for developing a chronic health condition should not be considered actionable if the only potential value of that result is to motivate patients to make changes in their health behaviors. Since the empirical research currently available on this question is equivocal, we explore relevant psychological theories of human motivation to demonstrate that current theory does not support the assumption that information about genetic risk will be motivating to most patients in their attempts to make changes in health behaviors.

The effect of substantiated and unsubstantiated investigations of child maltreatment and subsequent adolescent health

Children with substantiated child maltreatment (CM) experience adverse health outcomes. However, it is unclear whether substantiation vs. an investigation not resulting in substantiation has a greater impact on subsequent adolescent health. Propensity scores were used to examine the effect of investigated reports on the subsequent health of 503 adolescent females. CM was categorized into three levels: 1) investigated and substantiated, 2) investigated but unsubstantiated, and 3) no investigation. Models using inverse propensity score weights estimated the effect of an investigation on subsequent teen motherhood, HIV-risk behaviors, drug use, and depressive symptoms. Females with any investigation, regardless of substantiation status, were more likely to become teen mothers, engage in HIV-risk behaviors, and use drugs compared to females with no investigated report. Substantiated CM was associated with depressive symptoms. Findings underscore the importance of maintaining case records, regardless of substantiation, to better serve adolescents at risk for deleterious outcomes. Prospective methods and propensity scores bolster causal inference and highlight how interventions implemented following investigation are an important prevention opportunity.

Provider-Level Characteristics Associated With Adolescent Varicella, Meningococcal, and Human Papillomavirus Immunization Initiation

Purpose. We examined patient- and provider-level factors associated with initiation of three adolescent immunizations among 13 to 18 year olds in an adolescent primary care clinic. Methods. Data were extracted retrospectively from medical records. Logistic regression models identified associations with immunization initiation. Post hoc analyses stratified by gender were conducted to examine provider-type contribution to human papillomavirus (HPV) immunization initiation. Results. Among 2932 adolescents, rates of meningococcal and varicella immunization initiation differed by age. Girls were more likely to have initiated HPV immunization than boys. The probability of girls initiating HPV immunization was the same when last seen by advanced practice nurses (APNs) versus physicians, but the probability of boys initiating HPV immunization was lower when last seen by APNs. Conclusions. Differences in HPV immunization initiation were observed between genders, and for boys, between APN versus physician at last clinic visit. This may reflect changes to HPV immunization recommendations for boys and APNs having shorter clinic visits.