Satoshi Shiraishi

Effects of temperature on development and growth in the tick, Haemaphysalis longicornis

As a part of ecological studies onHaemaphysalis longicornis, the effects of controlled temperatures (12, 15, 20, 25 and 30°C; ∼ 100% RH) on development and growth of the tick were investigated and the critical low temperature for each stage in the life cycle was estimated. As the temperature became low, the periods of preoviposition, oviposition, egg hatching (incubation) and moulting were prolonged. At 12°C, however, oviposition, egg hatching and moulting of the larva and nymph did not occur. The critical low temperatures for oviposition, egg hatching (developmental zero) and larval and nymphal moulting which were calculated theoretically from the regression equations, were 11.1, 12.2, 10.2 and 11.8°C, respectively. The temperature also affected the egg productivity and hatch-ratio. The number of deposited eggs per mg of body weight decreased markedly at 15°C, and the hatch-ratio was lowered with dropped temperatures.

Functional morphology of Gené's organ inHaemaphysalis longicornis (Acari: Ixodidae)

The structure and function of Genés organ, which secretes the egg wax, were investigated inHaemaphysalis longicornis. The hatching rates of eggs deposited before and after the prevention of the eversion of Genés organ were compared at the conditions of 30°C, 100% RH and soaking in liquid paraffin. The movement of Genés organ was observed, and the structures of this organ in unfed and ovipositing females were examined with both light and scanning electron microscopes.The hatching rates of eggs without contact with Genés organ were markedly lower (4.9%) than that of normal eggs (94.5%) even at the suitable condition for eggs (30°C, 100% RH). In contrast, the hatching rates of eggs soaked in liquid paraffin without contact of Genés organ were considerably higher (71.8%) than that of eggs without both Genés organ contact and soaking in liquid paraffin.When oviposition commenced, the camerostome of the female was widened by a downswing of the capitulum, the paired horns of Genés organ emerged through it, and the oviposited egg from the genital aperture was coated with egg wax. At the same time of retraction of the horns which grip an egg, the capitulum was swung up and the egg was loaded on the scutum.Genés organ included the outer and inner cuticles, and the outer one is highly folded like bellows when the horns of this organ were retracted into the haemocoel. The undeveloped glands lying in the peripheral region of Genés organ in the unfed stage commenced their enlargement at the time of the onset of feeding, and they became large tubular glands in the 1-day ovipositing female. The columnar glandular cells of the tubular glands extended their domed apical region into the lumen and contained numerous secretory granules during oviposition.These results suggest that the tubular glands secrete the egg wax which is indispensable for egg survival by serving as a waterproofing agent.

Growth, development, and reproductive patterns in the Japanese field vole, Microtus montebelli

We describe characteristics related to the reproductive biology of the Japanese field vole, Microtus montebelli . In comparison with the other species of Microtus examined to date, Japanese field voles grew more rapidly and exhibited the most male-biased sexual dimorphism, with males having relatively small testes, indicative of a polygynous mating system. Thus, we conclude that this species is polygynous, possibly with the type of facultatively monogamous mating in which species show social patterns that combine elements of several systems.

Feeding Behaviour of Three Species of Squirrels

The Japanese giant flying squirrel, Petaurista leucogenys, Japanese flying squirrel, Pteromys momonga and Asiatic chipmunk, were compared for feeding behaviour under experimental conditions. Although P. leucogenys always fed in a sitting posture on branches, it extended its forepaws effectively both laterally and ventrally. When the animal was not able to place its large body on a slender twig, it brought the twig within reach of the mouth by the forepaws, obtaining food at the tip. P. momonga and T. s. asiaticus took a hanging posture, too, and the former was more skillful in this posture than was the latter. In picking up food scattered on the floor, P. leucogenys extended the body, without shifting its hindfeet, in a wide range including its lateral side; T. s. asiaticus got food in a narrow range only just in front of the body, and P. momonga had an intermediate range between those of the above two species. Among the three species, the daily frequency of feeding bouts was in inverse proportion to the duration of a feeding bout; P. leucogenys had relatively long and infrequent bouts, the reverse being the case with T. s. asiaticus. Characteristics of the posture, technique and frequency of feeding in the three species were supposed to be a reflection of interspecific differences in the diet, habitat, and freedom and length of the forelimb.

Ultrastructure of receptaculum seminis in Haemaphysalis longicornis (Acari: Ixodidae) in relation to inserted endospermatophore

The receptaculum seminis, opening into the female genital tract, is found only in the metastriate ixodid ticks. An endospermatophore that has been inserted into the female genital aperture at copulation is first stored in the receptaculum seminis, where spermiogenesis is completed before the sperm ascend the oviducts. The receptaculum seminis consists of a simple cuticularized epithelium. Epithelial cells in sexually matured females develop during feeding and become active in secretion. Secretions discharged from epithelial cells are released into the lumen of this organ through the cuticle and may act on the wall of the inserted endospermatophore. The fact that resumption of spermiogenesis (spermateleosis) has already occurred before destruction of the endospermatophore just after copulation suggests that secretions from epithelial cells of the receptaculum seminis are not the trigger of spermateleosis, but a destructive agent of the endospermatophore wall. J Morphol 231:143–147, 1997.

Ultrastructure of the tubular accessory gland in Haemaphysalis longicornis (Acari: Ixodidae)

The paired tubular accessory glands in Haemaphysalis longicornis open at the junction of the cervical and the vestibular parts of vagina via short and narrow ducts. The pseudostratified columnar glandular epithelium covered by the muscle layer consists of both secretory and supporting cells. As feeding proceeds, the secretory cells increase in volume. In ovipositing females, well‐developed rough endoplasmic reticulum, the Golgi complex, and membranebound granules that are undergoing exocytosis suggest that the secretory cells are involved in protein synthesis. However, in virgin females that fed 10 days, only small dense granules and no secretion activity were observed. The secretions from the tubular accessory gland may be released into the genital tract during the egg passage through the vagina. However, the supporting cells located between the secretory cells become slender during feeding, cohere to each other at the luminal side, and have a very narrow attachment at the basement membrane. Supporting cells probably help maintain secretory cell shape especially during granular discharge into the lumen.