Scott S. Howard

Room-temperature continuous-wave quantum cascade lasers grown by MOCVD without lateral regrowth

We report on room-temperature continuous-wave (CW) operation of lambda~8.2 mum quantum cascade lasers grown by metal-organic chemical vapor deposition without lateral regrowth. The lasers have been processed as double-channel ridge waveguides with thick electroplated gold. CW output power of 5.3 mW is measured at 300 K with a threshold current density of 2.63 kA/cm2. The measured gain at room temperature is close to the theoretical design, which enables the lasers to overcome the relatively high waveguide loss

Frequency Multiplexed In Vivo Multiphoton Phosphorescence Lifetime Microscopy

Multiphoton microscopy (MPM) is widely used for optical sectioning deep in scattering tissue, in vivo [1–2]. Phosphorescence lifetime imaging microscopy (PLIM) [3] is a powerful technique for obtaining biologically relevant chemical information through Förster resonance energy transfer and phosphorescence quenching [4–5]. Point-measurement PLIM [6] of phosphorescence quenching probes has recently provided oxygen partial pressure measurements in small rodent brain vasculature identified by high-resolution MPM [7, 8]. However, the maximum fluorescence generation rate, which is inversely proportional to the phosphorescence lifetime, fundamentally limits PLIM pixel rates. Here we experimentally demonstrate a parallel-excitation/parallel collection MPM-PLIM system that increases pixel rate by a factor of 100 compared with conventional configurations while simultaneously acquiring lifetime and intensity images at depth in vivo. Full-frame three-dimensional in vivo PLIM imaging of phosphorescent quenching dye is presented for the first time and defines a new platform for biological and medical imaging.

In vivo imaging of unstained tissues using long gradient index lens multiphoton endoscopic systems

We characterize long (up to 285 mm) gradient index (GRIN) lens endoscope systems for multiphoton imaging. We fabricate a portable, rigid endoscope system suitable for imaging unstained tissues, potentially deep within the body, using a GRIN lens system of 1 mm diameter and 8 cm length. The portable device is capable of imaging a ~200 µm diameter field of view at 4 frames/s. The lateral and axial resolution in water is 0.85 µm and 7.4 µm respectively. In vivo images of unstained tissues in live, anesthetized rats using the portable device are presented. These results show great promise for GRIN endoscopy to be used clinically.

Mode tuning of quantum cascade lasers through optical processing of chalcogenide glass claddings

In this letter, we demonstrate a method of tuning quantum cascade (QC) lasers by modifying the optical properties of an overlying cladding material. An amorphous chalcogenide cladding layer is deposited through a low temperature, solvent-casting technique that is compatible with current QC laser fabrication and operation. Above band gap illumination (λ<530nm) of this cladding causes a permanent change in its index of refraction leading to a change of the modal refractive index and a corresponding modal shift in the laser. Combined with deep-etched distributed Bragg gratings, a tuning of over 30nm is obtained at an operating wavelength of 7.9μm for constant current and temperature.

High-Performance Quantum Cascade Lasers: Optimized Design Through Waveguide and Thermal Modeling

We present a comprehensive model to study the thermal effects in quantum cascade (QC) lasers for continuous-wave (CW) operation at and above room temperature. This model self-consistently solves the temperature-dependent threshold current density equation and heat equation to determine the CW threshold current density, maximum heat sink temperature, and core temperature at threshold for a given laser design. The model includes effects from temperature dependence on thermal backfilling, thermal conductivity, phonon lifetimes, gain bandwidth, thermionic emission, and resistive heating in waveguide layers. Studies on these effects yield results not simultaneously considered by previous models. By including these results in laser designs, lasers with lower core temperatures, with higher operating temperatures, and requiring lower electrical power than current high-performance lasers are predicted. Additionally, experimental results are presented, exploring various methods of improving CW laser performance for a lambda ~ 8 mum QC laser and are compared to the model.

Thermal and Stark-Effect Roll-Over of Quantum-Cascade Lasers

A computational and experimental analysis of rollover in high-performance lambda ~ 8 mum quantum-cascade (QC) lasers is presented. In addition to conventional, thermal rollover, which is also a common cause of power rollover in diode lasers, ;Stark-effect; rollover is observed. While both effects can occur in the same QC laser design, thermal and Stark-effect rollover are shown to be the dominating factor for high-temperature continuous wave operated, and pulsed low-temperature operated and low-doped lasers, respectively. Additionally, the role of the continuum above the wells and barriers is discussed for both effects.

Midinfrared semiconductor optical metamaterials

We report on a novel class of semiconductor metamaterials that employ a strongly anisotropic dielectric function to achieve negative refraction in the midinfrared region of the spectrum, ∼8.5–13u2002μm. We present two types of metamaterials, layered highly doped/undoped heterostructures and quantum well superlattices that are highly anisotropic. Contrary to other optical metamaterials these heterostructure systems are optically thick (up to 20u2002μm thick), planar, and require no additional fabrication steps beyond the initial growth. Using transmission and reflection measurements and modeling of the highly doped heterostructures, we demonstrate that these materials exhibit negative refraction. For the highly doped quantum well superlattices, we demonstrate anomalous reflection due to the strong anisotropy of the material but a determination of the sign of refraction is still difficult. This new class of semiconductor metamaterials has great potential for waveguiding and imaging applications in the long-wave inf...

A quantum cascade laser cw cavity ringdown spectrometer coupled to a supersonic expansion source

A new instrument has been constructed that couples a supersonic expansion source to a continuous wave cavity ringdown spectrometer using a Fabry-Perot quantum cascade laser (QCL). The purpose of the instrument is to enable the acquisition of a cold, rotationally resolved gas phase spectrum of buckminsterfullerene (C(60)). As a first test of the system, high resolution spectra of the nu(8) vibrational band of CH(2)Br(2) have been acquired at approximately 1197 cm(-1). To our knowledge, this is the first time that a vibrational band not previously recorded with rotational resolution has been acquired with a QCL-based ringdown spectrometer. 62 transitions of the three isotopologues of CH(2)Br(2) were assigned and fit to effective Hamiltonians with a standard deviation of 14 MHz, which is smaller than the laser frequency step size. The spectra have a noise equivalent absorption coefficient of 1.4 x 10(-8) cm(-1). Spectral simulations of the band indicate that the supersonic source produces rotationally cold (approximately 7 K) molecules.

Quantum cascade lasers with voltage defect of less than one longitudinal optical phonon energy

Efficient use of applied voltage in quantum cascade (QC) lasers is a critical factor in achieving high wall-plug efficiency and low compliance voltage. We demonstrate a QC laser emitting at 4.2u2002μm featuring a low voltage defect and short injector with only four quantum wells. Devices with a voltage defect of 20 meV, well below the energy of the longitudinal optical phonons, and a voltage efficiency of 91%, a record value for QC lasers, are reported for pulsed operation at 180 K. Voltage efficiencies of greater than 80% are exhibited at room temperature. Overall performance showed wall-plug efficiencies ranging from 21% at cryogenic temperatures to 5.3% at room temperature.

Two-photon fluorescence imaging of intracellular hydrogen peroxide with chemoselective fluorescent probes

Abstract. We present the application of two-photon fluorescence (TPF) imaging to monitor intracellular hydrogen peroxide (H2O2) production in brain cells. For selective imaging of H2O2 over other reactive oxygen species, we employed small-molecule fluorescent probes that utilize a chemoselective boronate deprotection mechanism. Peroxyfluor-6 acetoxymethyl ester detects global cellular H2O2 and mitochondria peroxy yellow 1 detects mitochondrial H2O2. Two-photon absorption cross sections for these H2O2 probes are measured with a mode-locked Ti:sapphire laser in the wavelength range of 720 to 1040 nm. TPF imaging is demonstrated in the HT22 cell line to monitor both cytoplasmic H2O2 and localized H2O2 production in mitochondria. Endogenous cytoplasmic H2O2 production is detected with TPF imaging in rat astrocytes modified with d-amino acid oxidase. The TPF H2O2 imaging demonstrated that these chemoselective probes are powerful tools for the detection of intracellular H2O2.