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Immunohistochemical evaluation of experimental Vagococcus salmoninarum infection in rainbow trout (Oncorhynchus mykiss, Walbaum 1792)

The aim of this study was to investigate the pathogenesis and histopathological and immunohistochemical findings in rainbow trout (Oncorhynchus mykiss) following experimental vagococcosis. For this purpose, 60 rainbow trout were used. The experimental study used the pathogen Vagococcus salmoninarum. The fish were intraperitoneally (IP) administered with an inoculate containing 0.1 mL of the bacteria, resulting in a dose of 1.2 × 10(9) cfu mL(-1) per fish. For histopathological observations, tissue samples were taken from fish that died during the experiment and fish that survived until the end of the trial (60th day). All the tissue samples were immunohistochemically stained by the avidin-biotin-peroxidase complex and immunofluorescence methods using polyclonal antibody to detect V. salmoninarum antigens. In immunoperoxidase staining, positive reactions to bacterial antigens were most commonly seen in the kidney, heart and liver. In the immunofluorescence analysis, the distribution of antigens in the tissue and organs was similar to that observed with the immunoperoxidase staining. The results reveal an important correlation between histochemical and immunohistochemical staining in demonstrating the distribution of V. salmoninarum antigens in the affected tissues.

Bazı Bakteriyel Balık Patojenlerine Karşı Bazı Bitkisel Uçucu Yağlarının Antibakteriyel Aktivitesi

B u ara stirmada, karanfil ( Eugenia caryophyllata ), nane ( Menta piperita ) ve lavanta ( Lavandula hybrida ) ucucu yaglarinin kimyasal bilesimleri ve bakteriyel balik patojenler, Aeromonas sobria, Aeromonas salmonicida achromogenes, Aeromonas caviae, Aeromonas hydrophila, Vibrio anguillarum, Vibrio ordalli, Vibrio alginolyticus , Yersinia ruckeri , Flavobacterium psychrophilum, Lactococcus garvieae ve Vagacoccus salmoninarum ’a karsi in vitro a ntibakteriyel etkileri arastirilmistir. Sonuc olarak, karanfil ucucu yaginin guclu antibakteriyel etki gosterdigi tespit edilmistir. Bununla birlikte, nane ve lavanta yaglari orta derecede etkili bulunmustur.

Ichthyobodo spp. Infection in Meagre (Argyrosomus regius) from Turkey: Parasitological and Pathological Findings.

OBJECTIVE The aim of this study is to describe the first report of Ichthyobodo spp. infection in meagre (Argyrosomus regius) fry in a marine aquaculture facility in Turkey. METHODS The material of the study was composed of 30 meagre A. regius in 2-3 g weight taken from the fry adaptation unit of a fish farm in the Aegean Sea. In this study, parasitological and pathological examinations were performed on the meagre. Ichthyobodo spp. was determined on the body surfaces and gills. RESULTS Pathological examination revealed grayish mucous and erosions between the pin head and lentin over the skin of the examined specimens. Microscopic examinations revealed significant spongiosis, vacuolar degeneration, and hyperplasia in epidermal malpighian cells and hyperplasia in goblet cells. CONCLUSION In the present study, Ichthyobodo spp. infection was for the first time determined in an alternative cultured meagre in Turkey.