Selçuk Duman

Treatment of intestinal reperfusion injury using antioxidative agents

BACKGROUND/PURPOSE A number of antioxidants are present in human tissues, which comprise the secondary defence system against the oxygen-free radicals and oxidative stress. Therefore, this study was undertaken in an animal model to investigate the effects of antioxidants such as vitamin E, vitamin C, methyl prednisolone, and mannitol, on the treatment of intestinal reperfusion injury when given during ischemia. METHODS The study was performed on six groups of animals, each composed of six rabbits: Group I, mesenteric ischemia group; group R, ischemia-reperfusion group; group E, ischemia-reperfusion plus Vitamin E treatment; group C, ischemia-reperfusion plus vitamin C treatment; group CS, ischemia-reperfusion plus corticosteroid treatment; and Group M, ischemia-reperfusion plus mannitol treatment. Malondialdehyde (MDA) and glutathione (GSH) levels of the intestinal mucosa were assayed to reflect the free radical formation. RESULTS Mucosal injury scores in the M and C groups were significantly lower than the R group (P< .01 and P< .01, respectively), and in the E and CS groups, mucosal injury scores were not significantly different from R group. MDA levels in the M and C groups were significantly lower than the R group (P < .01 and P < .01, respectively). GSH levels in the E, C, and M groups, were significantly higher than R group (P < .01, P < .05, and P < .01, respectively). CONCLUSION The results of this study showed that antioxidant drugs, such as vitamin C and mannitol, may play a role in attenuating reperfusion injury of the gut demonstrated by depression of tissue MDA levels and by elevation of tissue GSH levels.

Comparison of Pregnancy Outcomes of Progesterone or Progesterone + Estradiol for Luteal Phase Support in ICSI-ET Cycles

Objective: To find out the effect of estradiol with progesterone for luteal phase support in IVF-ICSI cycles. Materials and Methods: Patients were accepted for treatment in the ART unit of Selcuk University, Meram Faculty of Medicine, between January 2001 and March 2003. The study was done in a prospective manner. The age range of 252 women was 19–41 years and the total number of cycles was 310. All patients were treated with a long ovulation induction protocol. Patients were treated and divided into two groups in a randomized manner: group I used only 600 mg/day divided into three equal doses of micronized progesterone vaginally, and group II used transdermal estradiol 100 µg/day + 600 mg/day vaginal micronized progesterone. Results: 310 ICSI cycles were carried out in 252 infertile couples between January 2001 and March 2003. From 22 of these cycles, oocytes were retrieved but no embryos were developed. In the remaining 288 cycles there were embryo transfers. All embryo development was achieved by ICSI treatment. In 148 out of 288 cycles, the luteal phase was supported only by vaginal micronized progesterone (group I). On the other hand, the remaining 140 cycles received vaginal micronized progesterone plus transdermal estradiol 100 µg/day (group II). The number of β-hCG-positive results in group I and group II were 20 (13.5 %) and 54 (38.5%) respectively. Conclusion: Adding estradiol to progesterone for luteal phase support in ICSI-ET cycles may increase implantation and pregnancy rates.

Intra-articular platelet-rich plasma injection for the treatment of temporomandibular disorders and a comparison with arthrocentesis.

BACKGROUND Temporomandibular joint (TMJ) internal derangements are progressive painful conditions and cause joint dysfunction, joint sound, malocclusion, and locking of the mouth. Conservative and invasive techniques can be used for the treatment of TMJ internal derangements. The objective of the present study was to examine the benefit of an intra-articular platelet-rich plasma (PRP) injection and to compare this with arthrocentesis. METHODS Twenty patients (female: male; 15:5; age 26, 3 ± 9.3 years) for a total of 32 joints with reducible anterior disc dislocation, as confirmed by Magnetic Resonance Imaging (MRI), were divided into two groups. PRP was used for the study group, and arthrocentesis was used for the control group. Pain intensity, maximal interincisal opening, and TMJ sounds were assessed and compared for evaluation of treatment success. RESULTS There was a statistically significant reduction in pain intensity and joint sound and an increase in mouth opening in the study group when compared with the control group. CONCLUSIONS This study shows that intra-articular PRP injection for the treatment of reducible disc displacement of the TMJ is a more effective method than arthrocentesis.

Assessment of Spermatozoa Morphology under Light Microscopy with Different Histologic Stains and Comparison of Morphometric Measurements

El objetivo fue examinar la morfologia de los espermatozoides con diferentes metodos de tincion y encontrar los mejores metodos para su estudio. Fueron seleccionados para el estudio, de manera aleatoria 67 pacientes, quienes ingresaron a la Unidad de tecnicas de reproduccion asistida. En la primera parte del estudio, se realizaron y tineron frotis con hematoxilina eosina (HE), Azul de Toluidina (AT), Giemsa, tincion de Wright, Hematoxilina Ferrica de Weigert, Anaranjado G, tincion eosina-anilina, metodo de Shorr, Papanicolau, metodo Berg, tincion verde brillante, anaranjado de acridina (AO) y tincion verde Janus. En la segunda parte del estudio, se realizaron frotis de 10 pacientes con normozoospermicos y se tineron con HE, AT, Metodo Shorr y Papanicolau. Se realizaron cuatro mediciones: ancho de la cabeza, longitud de la cabeza, parte media y cola, sobre 200 espermatozoides con morfologia normal. Se compararon las tinciones que mostraban mejor la morfologia. La evaluacion de la compactacion no fue posible en los frotis tenidos con los metodos de Shorr, Berg y AO. Una mejor evaluacion de la copactacion podria hacerse en otras tinciones. En la segunda parte los valores menores correspondieron a la tincion de AT en relacion a la medicion de la cabeza de los espermatozoides. Hubo una diferencia significativa en la longitud de la cabeza con tincion de AT. Las mediciones en los frotis con tecnicas de Shorr y Papanicolau fueron similares, con valores mas altos bajo tincion de Papanicolau. Se concluyo que los valores de la medicion morfologica en espermatozoides humanos podrian ser alterados segun el metodo de tincion utilizado.

Influence of cumulus cell coculture and cumulusaided embryo transfer on embryonic development and pregnancy rates.

OBJECTIVE In this study, we aimed to evaluate the influence of autologous cumulus oocyte complex (COC) coculture on embryonic development and quality, and investigate the implantation and pregnancy rates after cumulus-aided embryo transfer in the ICSI-ET cycles. MATERIAL AND METHODS Ninety five consecutive infertile women undergoing their first cycle of IVF teratment were included in the study. The cases were divided into two groups. Group 1 consisted of 48 women undergoing ICSI, along with autologous cumulus embryo coculture and cumulus-aided emryo transfer. Group 2 comprised 47 consecutive patients who consented to undergo ICSI and in whom autologous cumulus embryo coculture and cumulus-aided embryo transfer were not performed. Implantation and pregnancy rates were compared between the two groups. RESULTS The demographic data and controlled ovarian hyperstimulation parameters were similar in the two groups. The fertilization and cleavage rates were found to be higher in group 1 when compared with group 2 (p=0.03 and 0.001, respectively). There were no statistical significant differences for the implantation and clinical pregnancy rates between the two groups. CONCLUSION Usage of autologous COCs as coculture may improve fertilization and cleavage rates. However, cumulus-aided embryo transfer does not produce an increase in implantation and pregnancy rates.

Effects of Lipokit® Centrifugation on Morphology and Resident Cells of Adipose Tissue

DUMAN, S.; AKTAN, T. M.; CUCE, G.; CIHANTIMUR, B.; TOKAC, M. & AKBULUT, H. Effects of Lipokit® centrifugation onmorphology and resident cells of adipose tissue. Int. J. Morphol., 31(1):64-69, 2013.SUMMARY: The aim of adipose tissue engineering is creating autologus vascularized fat tissue to be used for practical softtissue reconstruction in human clinic. Unfortunately, in practice, long-term results of fat transplantation are often untrustworthy andunreliable, to overcome this problem different many lipoinjection techniques developed in the last 20 years. Centrifuge is a fu ndamentalstep in the preparation of adipose tissue. We focused on some cell markers especially MSCs markers and histological structural p ropertiesafter with lipokit centrifugation and without lipokit centrifugation of adipose tissue obtained by liposuction by this new technique.Adipose tissue was taken by liposuction and separates to two portions. One of them is centrifugated by Lipokit machine (C+) has a microfilter and the other is not (C-). After centrifugation smear slides and paraffin sections were prepared from these tissues. The se slides werestained with HE Stem cell; Angiogenesis.

Autologous Transplantation of Arterial Cells Improves Cardiac Function in a Rabbit Model of Infarcted Myocardium

Cellular cardiomyoplasty is a promising approach for the treatment of severe heart failure. However, the question which cell line is the best to use is still a matter of debate. In this study, we aimed to evaluate the efficacy of arterial media-intima cell suspension (AMICS) transplantation in rabbit myocardial infarct model. The study was divided into 2 groups: group A (the cell-treated group, n = 9) and group B (the medium injection group, n = 8). Group A was further divided into 2 subgroups as branch-1 (treated with unlabeled cells) and branch-2 (treated with iron-labeled cells). The experimental myocardial infarction (MI) was induced by ligation of left anterior descending coronary artery with a combination of cryoinjury. Ten days after the MI, cells obtained from autologous femoral arteries were injected into the injured myocardium of group A, while group B received an injection of only DMEM medium. Clinical, echocardiographic, and histopathologic evaluations were done. As compared to the ninth day values, echocardiography showed a significant improvement in systolic functions and left ventricular (LV) dimensions of the cell-treated group on the 30th day. In the heart biopsy sections of branch-1, the immunostained injected cells were observed to exist closely, suggesting an organization. Cells existing separately and lumen-like structure organizations stained positive with both smooth muscle cell (SMC) alpha-actin and Prussian Blue were also showed in the histological observation of branch-2. Autologous AMICS transplantation seems to be a feasible and efficacious method for cellular cardiomyoplasty in our rabbit model.

Cellular and Molecular Aspects of Adipose Tissue

Adipose tissue is a dynamic ‘hard-working’ tissue that awaits extensive studies. It does not merely function as a fat-storage region; adipose tissue also plays a major role in the formation of body shapes, which determine attractiveness in humans. Moreover, adipose tissue secretes molecules that direct brain processes.

Improvement in embryo quality and pregnancy rates by using autologous cumulus body during icsi cycles.

OBJECTIVE To determine whether the addition of intact cumulus cell mass (ICM) to both embryo culture (EC) and embryo transfer (ET) improves embryo quality and pregnancy rates. MATERIAL AND METHODS A total of 133 infertile couples were included, of which 67 received ICM (study group) and 66 did not (control group). The ICM was obtained from a simple cutting of the cumulus corona oocyte complex (CCOC). A case control study design was used. RESULTS The clinical characteristics of the two groups before the embryo culturing step were similar with respect to age, estradiol level on the day of hCG and endometrial thickness on the day of embryo transfer (p>0.05). On the other hand study group with ICM had higher number of high quality embryos (3.1±1.4 vs 2.4±1.1, p=0.03), higher implantation rate (53.7% vs 34.8%, p=0.02) and higher ultrasound confirmation of gestational sac and fetal heart beat as ongoing pregnancy rates (44.7% vs 27.2%, p=0.04) compared to the control group without ICM. CONCLUSION Addition of ICM improves embryo quality and pregnancy rates. This is a cost-and time-effective simple procedure that shows great promise for the improvement of infertility treatment.

Improvement in rabbit corneal cell suspension viability after freezing with Gingko Biloba extract.

We investigated whether the addition of Gingko Biloba extract (EGb 761) to rabbit corneal epithelial medium before cell freezing improved cell viability after freezing then thawing. After removal of corneas, they were treated with enzymes and the corneal epithelium was prepared as a single cell suspension in freezing media with or without EGb 761. After freezing for two weeks then thawing, a higher cell viability was found in the cornea cell suspensions which had been frozen pretreated with EGb 761 in the media. The improvement with corneal cell viability with EGb 761 pretreatment is postulated to be based on the antioxidant capacity of the plant extract.