Tahsin Murad Aktan

Intra-articular platelet-rich plasma injection for the treatment of temporomandibular disorders and a comparison with arthrocentesis.

BACKGROUND Temporomandibular joint (TMJ) internal derangements are progressive painful conditions and cause joint dysfunction, joint sound, malocclusion, and locking of the mouth. Conservative and invasive techniques can be used for the treatment of TMJ internal derangements. The objective of the present study was to examine the benefit of an intra-articular platelet-rich plasma (PRP) injection and to compare this with arthrocentesis. METHODS Twenty patients (female: male; 15:5; age 26, 3 ± 9.3 years) for a total of 32 joints with reducible anterior disc dislocation, as confirmed by Magnetic Resonance Imaging (MRI), were divided into two groups. PRP was used for the study group, and arthrocentesis was used for the control group. Pain intensity, maximal interincisal opening, and TMJ sounds were assessed and compared for evaluation of treatment success. RESULTS There was a statistically significant reduction in pain intensity and joint sound and an increase in mouth opening in the study group when compared with the control group. CONCLUSIONS This study shows that intra-articular PRP injection for the treatment of reducible disc displacement of the TMJ is a more effective method than arthrocentesis.

Assessment of Spermatozoa Morphology under Light Microscopy with Different Histologic Stains and Comparison of Morphometric Measurements

El objetivo fue examinar la morfologia de los espermatozoides con diferentes metodos de tincion y encontrar los mejores metodos para su estudio. Fueron seleccionados para el estudio, de manera aleatoria 67 pacientes, quienes ingresaron a la Unidad de tecnicas de reproduccion asistida. En la primera parte del estudio, se realizaron y tineron frotis con hematoxilina eosina (HE), Azul de Toluidina (AT), Giemsa, tincion de Wright, Hematoxilina Ferrica de Weigert, Anaranjado G, tincion eosina-anilina, metodo de Shorr, Papanicolau, metodo Berg, tincion verde brillante, anaranjado de acridina (AO) y tincion verde Janus. En la segunda parte del estudio, se realizaron frotis de 10 pacientes con normozoospermicos y se tineron con HE, AT, Metodo Shorr y Papanicolau. Se realizaron cuatro mediciones: ancho de la cabeza, longitud de la cabeza, parte media y cola, sobre 200 espermatozoides con morfologia normal. Se compararon las tinciones que mostraban mejor la morfologia. La evaluacion de la compactacion no fue posible en los frotis tenidos con los metodos de Shorr, Berg y AO. Una mejor evaluacion de la copactacion podria hacerse en otras tinciones. En la segunda parte los valores menores correspondieron a la tincion de AT en relacion a la medicion de la cabeza de los espermatozoides. Hubo una diferencia significativa en la longitud de la cabeza con tincion de AT. Las mediciones en los frotis con tecnicas de Shorr y Papanicolau fueron similares, con valores mas altos bajo tincion de Papanicolau. Se concluyo que los valores de la medicion morfologica en espermatozoides humanos podrian ser alterados segun el metodo de tincion utilizado.

Effects of Lipokit® Centrifugation on Morphology and Resident Cells of Adipose Tissue

DUMAN, S.; AKTAN, T. M.; CUCE, G.; CIHANTIMUR, B.; TOKAC, M. & AKBULUT, H. Effects of Lipokit® centrifugation onmorphology and resident cells of adipose tissue. Int. J. Morphol., 31(1):64-69, 2013.SUMMARY: The aim of adipose tissue engineering is creating autologus vascularized fat tissue to be used for practical softtissue reconstruction in human clinic. Unfortunately, in practice, long-term results of fat transplantation are often untrustworthy andunreliable, to overcome this problem different many lipoinjection techniques developed in the last 20 years. Centrifuge is a fu ndamentalstep in the preparation of adipose tissue. We focused on some cell markers especially MSCs markers and histological structural p ropertiesafter with lipokit centrifugation and without lipokit centrifugation of adipose tissue obtained by liposuction by this new technique.Adipose tissue was taken by liposuction and separates to two portions. One of them is centrifugated by Lipokit machine (C+) has a microfilter and the other is not (C-). After centrifugation smear slides and paraffin sections were prepared from these tissues. The se slides werestained with HE Stem cell; Angiogenesis.

Cellular and Molecular Aspects of Adipose Tissue

Adipose tissue is a dynamic ‘hard-working’ tissue that awaits extensive studies. It does not merely function as a fat-storage region; adipose tissue also plays a major role in the formation of body shapes, which determine attractiveness in humans. Moreover, adipose tissue secretes molecules that direct brain processes.

Improvement in embryo quality and pregnancy rates by using autologous cumulus body during icsi cycles.

OBJECTIVE To determine whether the addition of intact cumulus cell mass (ICM) to both embryo culture (EC) and embryo transfer (ET) improves embryo quality and pregnancy rates. MATERIAL AND METHODS A total of 133 infertile couples were included, of which 67 received ICM (study group) and 66 did not (control group). The ICM was obtained from a simple cutting of the cumulus corona oocyte complex (CCOC). A case control study design was used. RESULTS The clinical characteristics of the two groups before the embryo culturing step were similar with respect to age, estradiol level on the day of hCG and endometrial thickness on the day of embryo transfer (p>0.05). On the other hand study group with ICM had higher number of high quality embryos (3.1±1.4 vs 2.4±1.1, p=0.03), higher implantation rate (53.7% vs 34.8%, p=0.02) and higher ultrasound confirmation of gestational sac and fetal heart beat as ongoing pregnancy rates (44.7% vs 27.2%, p=0.04) compared to the control group without ICM. CONCLUSION Addition of ICM improves embryo quality and pregnancy rates. This is a cost-and time-effective simple procedure that shows great promise for the improvement of infertility treatment.

Cellular Cardiomyoplasty: Arterial Cells-Stem Cells Transplantation

Cardiovascular disease is the leading cause of mortality and morbidity all over the world. Current treatments in cardiovascular disease do not prevent the cell loss and have no contribution to regeneration, although these therapies provided an improvement in the survival. Cardiac repair by stem cells promises regenerating damaged myocardium by reconstructing the injured heart from its component parts. Up to date, variety of stem cells including BM mononuclear, adipocyte progenitor cells, fibroblasts, smooth and strait muscle cells, circulating progenitor cells, embryonic stem cells, cord blood cells etc., have been used for cardiac repair experimentally and some at clinically. There is no consensus which cell should be use for cardiac repair. We strongly suggest that vascular wall cells seem to be a good candidate for cardiomyoplasty.