Semra Kurucu

Antimicrobial activity of propolis samples from two different regions of Anatolia

Antimicrobial activity of two propolis samples from Kazan and Marmaris regions in Turkey were investigated by the disc diffusion method. Antimicrobial activity was tested with four different ethanolic extracts (30, 50, 70, and 96% ethanol) of each sample against seven Gram positive, four Gram negative bacteria and one fungus culture. The activity was found to be mainly due to caffeic acid and its esters. An isomeric mixture containing 3,3-dimethylallyl caffeate, and isopent-3-enyl caffeate was isolated from Kazan propolis samples.

HPLC method for the analysis of harmol, harmalol, harmine and harmaline in the seeds of Peganum harmala L.

A simple and sensitive method for separation and determination of harmol, harmalol, harmine and harmaline has been developed and validated. Harmol, harmalol, harmine and harmaline were separated using a Metasil ODS column by isocratic elution with flow rate 1.5 ml/min. The mobile phase composition was Isopropyl alcohol-Acetonitrile-Water-Formic acid (100:100:300:0.3) (v/v/v/v) and pH adjusted 8.6 with triethylamine. Spectrophotometric detection was carried out at 330 nm. The linear range of detection for harmol, harmalol, harmine and harmaline were between 9.375-250, 30.750-246, 31.250-500 and 31.000-248 microg/ml, respectively. The method described was suitable for the determination of harmol, harmalol, harmine and harmaline in the seeds of Peganum harmala L.

Chemical Constituents of the Essential Oils of Nepeta italica L. and Nepeta sulfuriflora P. H. Davis

The essential oils of Nepeta italica L. and N. sulfuriflora P. H. Davis (Lamiaceae), were prepared by hydrodistillation and studied by capillary GC and GC–MS, 28 constituents were identified. The essential oils consisted mainly of 1,8-cineole (80.8% in the oil of N. italica and 61.5% in that of N. sulfuriflora).

GC-MS analysis of propolis samples from two different regions of Turkey.

Silylated ethanolic extract of two propolis samples from Kazan and Marmaris regions in Turkey were investigated by capillary GC-MS. The compounds were characterized by comparison with library searches. Twenty four compounds from Kazan samples were identified, eight of them were new for propolis. Eighteen compounds from Marmaris samples were identified, two of them were new for propolis.

COMPOSITION OF THE ESSENTIAL OIL OF NEPETA NUDA L. SSP. ALBIFLORA (BOISS) GAMS

The composition of the essential oil of Nepeta nuda L. ssp. albiflora (Boiss.) Gams (Lamiaceae) was analysed by means of capillary GC and GC-MS. The main components, accounting for 75.1% of the oil, were 4aα,7α,7aα-nepetalactone (37.5%) and 4aα,7α,7aβ-nepetalactone (37.6%). The sesquiterpene fraction amounted to 14.2% and was dominated by caryophyllene oxide (4.4%).

Essential oil composition of Nepeta nuda L. ssp. nuda

The chemical composition of the essential oil of N. nuda L. ssp. nuda (Lamiaceae) was examined by capillary GC and GC–MS methods. The main components of the oil were β-caryophyllene oxide (21.8%), spathulenol (13.8%), allo-aromadendrene (9.0%) and β-caryophyllene (5.4%). Nepetalactones were not found.

Alkaloids from Vinca major

Abstract Chemical investigations of the aerial parts of Vinca major have resulted in the isolation of two new alkaloids, 10-methoxyperakine and vincawajine. Another alkaloid, 10-methoxyvinorine, is reported for the first time from this species. The structures were determined using spectroscopic techniques.

The Essential Oils of Rhus coriaria L. (Sumac)

ABSTRACT The essential oils obtained from the leaf, fruit pericarp and branch/bark of Rhus coriaria L. by hydrodistillation were examined by GC and GC/MS. Sixty-three constituents in the branch/bark oil, sixty-three constituents in the leaf oil and eighty-five constituents in the fruit pericarp oil were identified. The predominant compounds in the pericarp oil were limonene (0.17–9.49%), nonanal (10.77–13.09%) and (Z)-2-decenal (9.90–42.35%), while major components of the leaf oil were β-caryophyllene (0.33–16.95%) and a sesquiterpene hydrocarbon tentatively identified as patchoulane (3.08–23.87%). The major constituents of the branch/bark oil were β-caryophyllene (12.35–21.91%) and cembrene (10.71–26.50%). The composition of oils from two different phytogeographic regions showed variations.

Composition of the Essential Oil From Roots and Rhizomes of Valeriana phu L. Growing Wild in Turkey

Abstract The volatile constituents isolated from roots and rhizomes of Valeriana phu L. were investigated by GC and GC/MS (EI) analysis. The roots and rhizomes yielded 0.64% (v/w) essential oil on a dry weight basis. From the oil 70 compounds could be identified with a valerenal isomer (11.3%), valerianol (3.1%), patchouli alcohol (2.9%) and valeranone (2.2%) as the main components. One new component was isolated and identified as 1-hydroxy-1,11,11-trimethyldecahydrocyclopropane azulene-10-one for the first time in V. phu.

Preliminary Examination of the Composition of the Essential Oil From the Roots and Rhizomes of Valeriana alpestris Stev. Growing in Turkey

Abstract The volatile constituents from roots and rhizomes of Valeriana alpestris Stev., obtained from Van, Turkey, were investigated by GC and GC/MS analysis. The oil yield of the plant material was 0.2% (v/w) on a dry weight basis. From the oil 82 components (34.1%) of the total oil could be identified. Hexadecanoic acid (12%), decadienal (3.3%), thymol (1.9%), γ-terpinene (1.7%), and dimethoxybenzene (1.7%) were the main components.