Sergio Nicolaiewsky

Efeito do genótipo halotano e de diferentes sistemas de produção na qualidade da carne suína

The effect of halothane genotypes (heterozygous and dominant homozygous) and intensive rearing systems (indoor, wood shavings bedding and outdoor) on pork quality were determinated. Ninety six castrated male pigs were used for the trial. Identification of the halothane genotype was determined in blood samples using the DNA-test, based on the polymerase chain reaction (PCR) amplification of the critical region of the ryanodine receptor and subsequent restricion of the amplifield fragment by the restriction fragment lengh polymorfism (RFLP) technique. The pH at 45 minutes and 24 hours after slaughter was measured on the Longissimus dorsi and Semimembranosus muscles. At the laboratory, the Longissimus dorsi muscle was used for the evaluation of the color, marbling, drip loss, defrosting loss and cooking loss and shear force. The muscle of heterozygous pigs for the halothane gene had lower pH and higher drip loss and the incidence of the PSE condition in this genotype was three times higher. The rearing system did not affect the initial and ultimate pH or water holding capacity. Indoor reared pigs had greater frequency of PSE carcass. The interaction between halothane genotypes and rearing systems had no significant effect on any trait studied.

Influência do gene halotano sobre a qualidade da carne suína

O objetivo deste trabalho foi avaliar o efeito do gene halotano sobre as caracteristicas de qualidade da carne suina. Foram utilizadas 151 carcacas de suinos hibridos comerciais, sendo 93 carcacas com genotipo halotano normal (HalNN), 51 heterozigotas (HalNn) e 7 recessivas (Halnn). As medidas efetuadas foram peso da carcaca, refletância atraves da fibra optica GP4-Hennessy, temperatura muscular aos 45 minutos e pH aos 45 minutos e 24 horas apos o abate no musculo Longissimus dorsi, cor e identificacao do genotipo halotano em amostras de gordura atraves de PCR-RFLP. Houve diferenca significativa entre suinos HalNn e HalNN e entre Halnn e HalNN quanto ao pH inicial e a cor. Em relacao a temperatura muscular e pH, final nao houve diferenca significativa entre os genotipos. A frequencia de carcacas PSE foi mais elevada nos suinos Halnn e HalNn do que nos suinos HalNN (85,71; 58,82 e 36,56%, pelo pH inicial; 71,43; 47,06 e 17,20%, pela cor, respectivamente). A qualidade da carne de suinos Halnn e HalNn foi inferior a de suinos HalNN, em termos de pH e cor.

Utilização da fitase em dietas com ou sem farelo de arroz desengordurado para suínos em crescimento/terminação

The objective of this work was to compare the efficiency of different levels of phytase with the use of inorganic phosphate (dicalcium phosphate) when added to diets with or without defatted rice bran (DRB). One hundred and twenty eight pigs with average initial weight of 21.6 ± 2.4 kg were housed in collective boxes for the pig performance evaluation in the growing and finishing phase. The pigs were slaughtered with an average weight of 95.42 ± 5.3 kg and the metacarpus bones were collected to determine the calcium and phosphorus content. Eight treatments were used with four diets being formulated without DRB (corn-soybean meal) and the other four diets with DRB. The phytase levels added in the diets were 0, 750 or 1000 PU/kg of the diet. It was observed that adding phytase in diets without and with DRB improved the availability of the organic phosphorus. The addition of phytase at levels of 750 or 1000 PU/kg, with or without DRB in the diet, allows the withdraw of the inorganic phosphorus source from diet if considering pig performance. However, the bone characteristics are affected when the diets with phytase were not supplemented with a source of inorganic phosphorus.

Efeito do genótipo halotano e de diferentes sistemas de produção no desempenho produtivo e na qualidade da carcaça suína

The objective of this study was to evaluate the effect of the halothane genotypes (heterozygous and dominant homozygous) and intensive rearing systems (indoor, wood shavings bedding and outdoor) on growth performance, carcass and meat yield. Ninety six castrated male pigs were used for the trial. Identification of the halothane genotype was determined in blood samples using the DNA-test, based on the polymerase chain reaction (PCR) for amplification of the critical region of the ryanodine receptor and subsequent restriction of the amplified fragment by restriction fragment lengh polymorfism technique (RFLP). Growth and finishing performance were evaluated by weight gain, feed intake and feed efficiency. Carcass quality was evaluated by backfat and muscle depth, meat percentage through Hennessy Grading Probe and carcass weight. Twenty four hours after slaugther, ham and loin weight were recorded and the loin eye area was evaluated. The growth performance was similar for the two genotypes. The heterozygous pigs for the halothane gene had higher percentage of fat-free lean and less fat in the carcass. Outdoor reared pigs showed lower average daily gain and worst efficiency than pigs reared indoor. Carcasses of pigs reared under wood shavings bed had less fat-free lean and greater backfat thickeness. The interaction between halothane genotypes and rearing systems had no significant effect on any trait studied.

A CONTRIBUIÇÃO DO GENE HALOTANO SOBRE AS CARACTERÍSTICAS DE QUALIDADE DA CARNE SUÍNA

). Asmedidas efetuadas foram: espessura de toucinho e de musculo,percentagem de carne, peso da carcaca, pH aos 45 minutos e 24horas apos o abate, no musculo Longissimus dorsi, cor, perda deliquido por gotejamento e identificacao do genotipo halotano emamostras de gordura atraves de PCR-RFLP. Suinos Hal

Efeito da fitase em dietas com ou sem fosfato inorgânico para suínos em crescimento

ABSTRACT - The objective of this trial was to compare the efficiency of phytase with an inorganic phosphate (dicalcium phosphate) when added to diets with or without defatted rice bran (DRB). One hundred and twenty eight pigs (50% barrows and 50% gilts) were housed in collective cages to evaluate the animals performance in the growing phase, and to determine the digestibility of the calcium and phosphorus using chromium oxide as an indicator and the concentration of calcium and phosphorus in the coccyces vertebra of the animals. Eight treatments were used: four diets without DRB (corn-soybean meal) and four diets with 30% of DRB. Phytase levels of 0, 750 and 1000 PU/kg were added to both diets with or without DRB, and the third one without DRB presented 750 PU/kg without inorganic phosphate, while in the diets with DRB one of them was not supplemented neither with phytase and nor with inorganic phosphate. Diets with or without DRB supplementation showed the same efficiency on the performance of the animals as that ones supplemented with dicalcium phosphate. However, phytase reduced the excretion of calcium and phosphorus in the feces and in the bone composition of the animals, only when was supplemented in diets without DRB.