Seung-Kyun Moon

Modification of TiO2 nanotube surfaces by electro-spray deposition of amoxicillin combined with PLGA for bactericidal effects at surgical implantation sites

OBJECTIVE To fabricate the antibiotic-releasing coatings on TiO(2) nanotube surfaces for wide applications of implant and bone plate in medical and dental surgery, the optimal deposition time of amoxicillin/PLGA solution simultaneously performing non-toxicity and a high bactericidal effect for preventing early implant failures was found. MATERIALS AND METHODS FE-SEM, ESD and FT-IR were used for confirming deposition of amoxicillin/PLGA on the TiO(2) surface. Also, the elution of amoxicillin/PLGA in a TiO(2) nanotube surface was measured by a UV-VIS spectrophotometer. The bactericidal effect of amoxicillin on the TiO(2) nanotube surface was evaluated by using Staphylococcus aureus (S. aureus). The cytotoxicity and cell proliferation were observed by WST assay using MC3T3-E1 osteoblast cells. RESULTS The results indicated that the TiO(2) nanotube surface controlled by electro-spray deposition time with amoxicillin/PLGA solution could provide a high bactericidal effect against S. aureus by the bactericidal effect of amoxicillin, as well as good osteoblast cell proliferation at the TiO(2) nanotube surface without toxicity. CONCLUSIONS This study used electro-spray deposition (ESD) methodology to obtain amoxicillin deposition in nanotube structures of TiO(2) and found the optimal deposition time of amoxicillin/PLGA solution simultaneously performing non-toxicity and a high bactericidal effect for preventing early implant failures.

Antibacterial effect and cytocompatibility of nano-structured TiO2 film containing Cl

The aim of this study was to investigate the antibacterial effect and cytocompatibility of a nano-structured TiO2 film that contained Cl and had been coated onto commercially pure titanium. First, we prepared nano-structured TiO2 by anodization with hydrofluoric acid. Then, to confer an antibacterial effect, we performed a second anodization with NaCl solutions of different concentrations (0.5 M, 1 M, 2 M). The morphology, composition, and wettability of the surface were investigated by SEM, EDS, and a video contact angle measuring system. The antibacterial effect was evaluated by film adhesion method. And cytotoxicity was determined by the viability of MG-63 cells in a MTT assay. The SEM and EDS results showed that the TiO2 nano-structure containing Cl had successfully formed after the second anodization. The contact angle analysis showed that the anodized titanium had a hydrophilic character. The results of this in vitro investigation demonstrated that the TiO2 nano-structure film anodized in 1 M NaCl had an antibacterial effect and good cell compatibility.

Biological evaluation of micro-nanoporous layer on Ti–Ag alloy for dental implant

Abstract This study examined the biocompatibility of the micro-nanoporous layer formed on a titanium-silver (Ti–Ag) alloy. The porous layer was formed by grit-blasting and anodic oxidation. The surface of the porous layer was rougher and more hydrophilic compared to a simple machined specimen and the expressions of bone-related genes were greater for cells grown on the porous layer compared to that of cells cultured on a control surface. Also the bone-to-plate contact rate in vivo test was significantly improved for porous layer plate compare to simple machined specimen (P < 0.05). The porous layer on Ti–Ag alloy enhanced the peri-implant bone formation at the early healing stage and it was believed that this porous layer on the Ti–Ag alloy will be suitable for dental implant applications.

Characterization of hydroxyapatite containing a titania layer formed by anodization coupled with blasting

Abstract Objectives. The modification of dental implant surface by increasing the surface roughness or/and altering chemical composition have been attempted. Among them, hydroxyapatite (HA) coatings are typically bioactive. On the other hand, titania coatings have good corrosion resistance and biocompatibility. Therefore, the objective of this study was to fabricate HA containing a titania layer using an HA blasting and anodization method to benefit from the advantages of both, followed by surface characterization and biocompatibility. Materials and methods. HA blasting was performed followed by microarc oxidation (MAO) using various applied voltages (100, 150, 200, 250 V). For surface characterization, the microstructure of the surface, surface phase and surface roughness were observed. Bonding strength was measured using a universal testing machine and potentiodynamic corrosion testing was performed. Biocompatibility was evaluated based on bioactivity and cell proliferation test. Results. The porous titanium oxide-containing HA was formed at 150 and 200 V. These surfaces were a lower corrosion current compared to the titanium treated only with HA blasting. In addition, composite treated titanium showed a rougher surface and tighter bonding strength compared to the titanium treated only with MAO. Biocompatibility demonstrated that HA/Titania composite layer on titanium showed a rapid HA precipitation and also enhanced cell proliferation. Conclusions. These results suggested that HA containing titania layer on titanium had not only excellent physicochemical, mechanical and electrochemical properties, but also improved bioactivity and biological properties that could be applied as material for a dental implant system.

Effect of non-thermal plasma on loading of tetracycline combined with plga into titania nanotube

Summary form only given. Titania nanotube (TNT) is a tube shaped thin film that is fabricated by process known as the anodization and it has been known to exhibit excellent biocompatibility and high surface-to-volume ratio, which makes it ideal structure as a carrier for drugs. However, drug loading on TNT has been limited by narrow diameter of TNT and to overcome this problem, the present study attempted to determine whether plasma treatment of inner TNT surface enhances its hydrophilicity and resultant drug carrying properties.