Shigeyasu Amano

Studies on the Discrimination of Lymphocytes and Plasma Cells SUPPLEMENTS ON ADVOCATION OF THE “LYMPHOGONIA”-THEORY*

In the lymphatic gland of well sensitized rabbits, there locate three groups of pyroninophile cells stained with the Unna‐Pappenheims method. Namely, they are (1) germ centers, (2) sinus, especially the intermedially sinus and (3) lymphatic pulp near the medullary sinus.

Peculiarity of Leukemia and Cancer Viruses Based on Our Recent Investigations Utilizing Ultra‐thin Sections by Electronmicrography

Fowl leukosis was introduced primarily by ELLERMANN and BANG (1908), while chicken myxosarcoma by FUJINAMI (1909) and successively by Rous (1910) as diseases elicited by filtrable agents. Proliferating modes of these viruses, however, remain yet unclarified, except the only suggestive description of GAYLORD (1955) referring to Rous sarcoma. In respect to the viral tumor in mammals, on the other hand, C3H mammary cancer called general interest, and especially, electron micrographical studies made by DMOCHOWSKI (1955), BERNHARD (1955), SUZUKI (1957) revealed the attitude of the cancer virus in the host cells, though these findings are thought to be the only exceptional case in mammalian cancer. However, GROSS (1951, 1954, 1957) reported his interesting works concerning leukemia in AK mice to explain the viral origin of this disease by way of his varied experimental procedures, though the proliferating modus of the causative virus is not yet demonstrated in the cytoplasm of leukemic cells by electron micrograph of ultra-thin sections (AMANO, 1 957). Without any finer cytological knowledge of the host cells the modus of virus infection should not be argued, and especially in the case of cancer virus which necessitates long latent stage in cancerogenesis. Also it is absolutely necessary to make an observation of the modus of virus proliferation in the course of the disease by electron microscopy of ultrathin sections for the understanding of general pathology of the cancer virus. Since the establishment of our Institute in 1956, we took up this problem as a principal theme of our study.