Shin-ichiro Yasui
Tohoku University
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Publication
Featured researches published by Shin-ichiro Yasui.
Biochimica et Biophysica Acta | 2013
Tetsuichi Wazawa; Shin-ichiro Yasui; Nobuyuki Morimoto; Makoto Suzuki
We investigate the effects of urea and its derivatives on the ATPase activity and on the in vitro motility of chicken skeletal muscle actomyosin. Mg-ATPase rate of myosin subfragment-1 (S1) is increased by 4-fold by 0.3M 1,3-diethylurea (DEU), but it is unaffected by urea, thiourea, and 1,3-dimethylurea at ≤1M concentration. Thus, we further examine the effects of DEU in comparison to those of urea as reference. In in vitro motility assay, we find that in the presence of 0.3M DEU, the sliding speeds of actin filaments driven by myosin and heavy meromyosin (HMM) are significantly decreased to 1/16 and 1/6.6, respectively, compared with the controls. However, the measurement of the actin-activated ATPase activity of HMM shows that the maximal rate, Vmax, is almost unchanged with DEU. Thus, the myosin-driven sliding motility of actin filaments is significantly impeded in the presence of 0.3M DEU, whereas the cyclic interaction of myosin with F-actin occurs during the ATP turnover, the rate of which is close to that without DEU. In contrast to DEU, 0.3M urea exhibits only modest effects on both actin-activated ATPase and sliding motility of actomyosin. Thus, DEU has the effect of uncoupling the sliding motility of actomyosin from its ATP turnover.
生物物理 | 2013
Tetsuichi Wazawa; Shin-ichiro Yasui; Nobuyuki Morimoto; Makoto Suzuki
Seibutsu Butsuri | 2013
Tetsuichi Wazawa; Shin-ichiro Yasui; Nobuyuki Morimoto; Makoto Suzuki
生物物理 | 2012
Takuya Nakagawa; Shin-ichiro Yasui; Tetsuichi Wazawa; George Mogami; Makoto Suzuki
Seibutsu Butsuri | 2012
Takuya Nakagawa; Shin-ichiro Yasui; Tetsuichi Wazawa; George Mogami; Makoto Suzuki
生物物理 | 2011
Shin-ichiro Yasui; Altansukh Batnasan; Tetsuichi Wazawa; Noriyoshi Ishida; Takuya Nakagawa; Makoto Suzuki
生物物理 | 2011
Tomohiko Abe; Altansukh Batnasan; Shin-ichiro Yasui; George Mogami; Makoto Suzuki
Seibutsu Butsuri | 2011
Tomohiko Abe; Altansukh Batnasan; Shin-ichiro Yasui; George Mogami; Makoto Suzuki
Seibutsu Butsuri | 2011
Shin-ichiro Yasui; Altansukh Batnasan; Tetsuichi Wazawa; Noriyoshi Ishida; Takuya Nakagawa; Makoto Suzuki
生物物理 | 2010
Tatsuya Osada; Shin-ichiro Yasui; Tetsuichi Wazawa; George Mogami; Nobuyuki Morimoto; Makoto Suzuki