Shinichiro Terasaki

Brush cytology of Herpes Simplex virus infection in oral mucosa: Use of the ThinPrep® processor†

Evaluation was made of oral scraping specimen using Cytobrush from 11 patients with clinical suspicious lesions for herpes simplex virus (HSV) infection. In this study, oral brush cytology prepared using an automated smear apparatus (ThinPrep® method) has been applied. The smears prepared by ThinPrep® are equally well suited for screening purposes, producing highly cellular specimens with well‐preserved cytoplasm and nuclei. The results obtained on the 11 patients, four cytologic specimens, showed pathognomonic viral cellular changes of HSV infection. Viral isolation studies were performed on 10 of the 11 patients and ten specimens tested positive for HSV by culture. Of the ten HSV positive isolates, six were positive by either cytology or immunocytochemical staining. Of the two smears showing HSV positive cells, both smears were also confirmed by in situ hybridization (ISH) with a biotinylated clone DNA probe.

Coffin-Siris Syndrome

Abstract A 9-month-old boy with Coffin Siris syndrome is described. Characteristic features include eyebrow hypertrichosis, long eyelashes, flat nasal bridge, prominent philtrum, hypoplasia or absence of the distal phalanges, high palate, and enlarged gingiva. The childs condition worsened and he ultimately died of multiple organ failure at the age of 5 years and 3 months.