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Dive into the research topics where Shu Zhen Chong is active.

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Featured researches published by Shu Zhen Chong.


Journal of Experimental Medicine | 2013

Neutrophil mobilization via plerixafor-mediated CXCR4 inhibition arises from lung demargination and blockade of neutrophil homing to the bone marrow

Sapna Devi; Yilin Wang; Weng Keong Chew; Ronald Lima; Noelia A-González; Citra Nurfarah Zaini Mattar; Shu Zhen Chong; Andreas Schlitzer; Nadja Bakocevic; Samantha Chew; Jo Keeble; Chi Ching Goh; Jackson LiangYao Li; Maximilien Evrard; Benoit Malleret; Anis Larbi; Laurent Rénia; Muzlifah Haniffa; Suet-Mien Tan; Jerry Kok Yen Chan; Karl Balabanian; Takashi Nagasawa; Françoise Bachelerie; Andrés Hidalgo; Florent Ginhoux; Paul Kubes; Lai Guan Ng

The CXCR4 antagonist plerixafor augments frequency of circulating neutrophils via release from the lung and prevents neutrophil homing to the bone marrow.


Journal of Investigative Dermatology | 2016

Neutrophils Self-Regulate Immune Complex-Mediated Cutaneous Inflammation through CXCL2

Jackson LiangYao Li; Chun Hwee Lim; Fen Wei Tay; Chi Ching Goh; Sapna Devi; Benoit Malleret; Bernett Lee; Nadja Bakocevic; Shu Zhen Chong; Maximilien Evrard; Hideaki Tanizaki; Hwee Ying Lim; Bruce Russell; Laurent Rénia; Francesca Zolezzi; Michael Poidinger; Veronique Angeli; Ashley L. St. John; John E. Harris; Hong Liang Tey; Suet-Mien Tan; Kenji Kabashima; Wolfgang Weninger; Anis Larbi; Lai Guan Ng

Deposition of immune complexes (ICs) in tissues triggers acute inflammatory pathology characterized by massive neutrophil influx leading to edema and hemorrhage, and is especially associated with vasculitis of the skin, but the mechanisms that regulate this type III hypersensitivity process remain poorly understood. Here, using a combination of multiphoton intravital microscopy and genomic approaches, we re-examined the cutaneous reverse passive Arthus reaction and observed that IC-activated neutrophils underwent transmigration, triggered further IC formation, and transported these ICs into the interstitium, whereas neutrophil depletion drastically reduced IC formation and ameliorated vascular leakage in vivo. Thereafter, we show that these neutrophils expressed high levels of CXCL2, which further amplified neutrophil recruitment and activation in an autocrine and/or paracrine manner. Notably, CXCL1 expression was restricted to tissue-resident cell types, but IC-activated neutrophils may also indirectly, via soluble factors, modulate macrophage CXCL1 expression. Consistent with their distinct cellular origins and localization, only neutralization of CXCL2 but not CXCL1 in the interstitium effectively reduced neutrophil recruitment. In summary, our study establishes that neutrophils are able to self-regulate their own recruitment and responses during IC-mediated inflammation through a CXCL2-driven feed forward loop.


Frontiers in Immunology | 2013

Lights, Camera, and Action: Vertebrate Skin Sets the Stage for Immune Cell Interaction with Arthropod-Vectored Pathogens

Shu Zhen Chong; Maximilien Evrard; Lai Guan Ng

Despite increasing studies targeted at host-pathogen interactions, vector-borne diseases remain one of the largest economic health burdens worldwide. Such diseases are vectored by hematophagous arthropods that deposit pathogens into the vertebrate host’s skin during a blood meal. These pathogens spend a substantial amount of time in the skin that allows for interaction with cutaneous immune cells, suggesting a window of opportunity for development of vaccine strategies. In particular, the recent availability of intravital imaging approaches has provided further insights into immune cell behavior in living tissues. Here, we discuss how such intravital imaging studies have contributed to our knowledge of cutaneous immune cell behavior and specifically, toward pathogen and tissue trauma from the arthropod bite. We also suggest future imaging approaches that may aid in better understanding of the complex interplay between arthropod-vectored pathogens and cutaneous immunity that could lead to improved therapeutic strategies.


Journal of Leukocyte Biology | 2015

Visualization of bone marrow monocyte mobilization using Cx3cr1gfp/+Flt3L-/- reporter mouse by multiphoton intravital microscopy.

Maximilien Evrard; Shu Zhen Chong; Sapna Devi; Weng Keong Chew; Bernett Lee; Michael Poidinger; Florent Ginhoux; Suet-Mien Tan; Lai Guan Ng

Monocytes are innate immune cells that play critical roles in inflammation and immune defense. A better comprehension of how monocytes are mobilized and recruited is fundamental to understand their biologic role in disease and steady state. The BM represents a major “checkpoint” for monocyte homeostasis, as it is the primary site for their production and release. Our study determined that the Cx3cr1gfp/+ mouse strain is currently the most ideal model for the visualization of monocyte behavior in the BM by multiphoton intravital microscopy. However, we observed that DCs are also labeled with high levels of GFP and thus, interfere with the accuracy of monocyte tracking in vivo. Hence, we generated a Cx3cr1gfp/+Flt3L−/− reporter mouse and showed that whereas monocyte numbers were not affected, DC numbers were reduced significantly, as DCs but not monocytes depend on Flt3 signaling for their development. We thus verified that mobilization of monocytes from the BM in Cx3cr1gfp/+Flt3L−/− mice is intact in response to LPS. Collectively, our study demonstrates that the Cx3cr1gfp/+Flt3L−/− reporter mouse model represents a powerful tool to visualize monocyte activities in BM and illustrates the potential of a Cx3cr1gfp/+‐based, multifunctionality fluorescence reporter approach to dissect monocyte function in vivo.


Immunity | 2018

Developmental Analysis of Bone Marrow Neutrophils Reveals Populations Specialized in Expansion, Trafficking, and Effector Functions

Maximilien Evrard; Immanuel W.H. Kwok; Shu Zhen Chong; Karen W.W. Teng; Etienne Becht; Jinmiao Chen; Je Lin Sieow; Hweixian Leong Penny; Goh Chi Ching; Sapna Devi; José M. Adrover; Jackson LiangYao Li; Ka Hang Liong; Leonard Tan; Zhiyong Poon; Shihui Foo; Jia Wang Chua; I-hsin Su; Karl Balabanian; Françoise Bachelerie; Subhra K. Biswas; Anis Larbi; William Ying Khee Hwang; Vikas Madan; H. Phillip Koeffler; Siew Cheng Wong; Evan W. Newell; Andrés Hidalgo; Florent Ginhoux; Lai Guan Ng

Summary Neutrophils are specialized innate cells that require constant replenishment from proliferative bone marrow (BM) precursors as a result of their short half‐life. Although it is established that neutrophils are derived from the granulocyte‐macrophage progenitor (GMP), the differentiation pathways from GMP to functional mature neutrophils are poorly defined. Using mass cytometry (CyTOF) and cell‐cycle‐based analysis, we identified three neutrophil subsets within the BM: a committed proliferative neutrophil precursor (preNeu) which differentiates into non‐proliferating immature neutrophils and mature neutrophils. Transcriptomic profiling and functional analysis revealed that preNeu require the C/EBP&egr; transcription factor for their generation from the GMP, and their proliferative program is substituted by a gain of migratory and effector function as they mature. preNeus expand under microbial and tumoral stress, and immature neutrophils are recruited to the periphery of tumor‐bearing mice. In summary, our study identifies specialized BM granulocytic populations that ensure supply under homeostasis and stress responses. Graphical Abstract Figure. No Caption available. HighlightsProliferation activity identifies committed neutrophil precursor in mice and humansNeutrophil subsets possess distinct transcriptomic and functional signaturesDefect in neutrophil development leads to impaired neutrophil‐mediated responsesIncreased circulating immature neutrophils are associated with cancer progression &NA; The neutrophil differentiation pathway is poorly defined. Evrard et. al. demonstrate a workflow of characterizing bone marrow neutrophil subsets on the basis of their proliferative capacity and molecular signatures and thereby define the developmental trajectory and functional properties of neutrophils.


European Journal of Immunology | 2018

The impact of ischemia-reperfusion injuries on skin resident murine dendritic cells

Chi Ching Goh; Maximilien Evrard; Shu Zhen Chong; Yingrou Tan; Leonard Tan; Karen Wei Weng Teng; Wolfgang Weninger; David L. Becker; Hong Liang Tey; Evan W. Newell; Bin Liu; Lai Guan Ng

Pressure ulcers are a chronic problem for patients or the elderly who require extended periods of bed rest. The formation of ulcers is due to repeated cycles of ischemia‐reperfusion (IR), which initiates an inflammatory response. Advanced ulcers disrupt the skin barrier, resulting in further complications. To date, the immunological aspect of skin IR has been understudied, partly due to the complexity of the skin immune cells. Through a combination of mass cytometry, confocal imaging and intravital multiphoton imaging, this study establishes a workflow for multidimensionality single cell analysis of skin myeloid cell responses in the context of IR injury with high spatiotemporal resolution. The data generated has provided us with previously uncharacterized insights into the distinct cellular behavior of resident dendritic cells (DCs) and recruited neutrophils post IR. Of interest, we observed a drop in DDC numbers in the IR region, which was subsequently replenished 48h post IR. More importantly, in these cells, we observe an attenuated response to repeated injuries, which may have implications in the subsequent wound healing process.


Current Opinion in Immunology | 2018

Illuminating the covert mission of mononuclear phagocytes in their regional niches

Shu Zhen Chong; Maximilien Evrard; Chi Ching Goh; Lai Guan Ng

Monocytes, dendritic cells (DCs) and macrophages have been classically categorized into the mononuclear phagocyte system (MPS) based on their similar functional and phenotypic characteristics. While an increasing amount of research has revealed substantial ontogenic and functional differences among these cells, the reasons behind their heterogeneity and strategic positioning in specific niches throughout the body are yet to be fully elucidated. In this review, we outline how recent advances in intravital imaging studies have dissected this phenomenon and have allowed us to appreciate how MPS cells exploit their regional niches to specialize and maximize their functional properties. Understanding their cellular behavior in each of their specialized microenvironment will eventually allow us to target specific cells and their behavioral patterns for improved vaccine and therapeutic purposes.


Journal of Experimental Medicine | 2016

CXCR4 identifies transitional bone marrow premonocytes that replenish the mature monocyte pool for peripheral responses.

Shu Zhen Chong; Maximilien Evrard; Sapna Devi; Jinmiao Chen; Jyue Yuan Lim; Peter See; Yiru Zhang; José M. Adrover; Bernett Lee; Leonard Tan; Jackson LiangYao Li; Ka Hang Liong; Cindy Phua; Akhila Balachander; Adrian Boey; David Liebl; Suet-Mien Tan; Jerry Chan; Karl Balabanian; John E. Harris; Mariaelvy Bianchini; Christian Weber; Johan Duchene; Josephine Lum; Michael Poidinger; Qingfeng Chen; Laurent Rénia; Cheng-I Wang; Anis Larbi; Gwendalyn J. Randolph


Archive | 2014

Technical Advance: Visualization of bone marrow monocyte mobilization using

Maximilien Evrard; Shu Zhen Chong; Sapna Devi; Weng Keong Chew; Bernett Lee; Michael Poidinger; Florent Ginhoux; Suet-Mien Tan; Lai Guan Ng


Archive | 2013

increasing VEGF-A bioavailability and secreting VEGF-D Neutrophils contribute to inflammatory lymphangiogenesis by

David M. Kemeny; Jean-Pierre Abastado; Veronique Angeli; Kar Wai Tan; Shu Zhen Chong; Fiona H. S. Wong; Maximilien Evrard; Sandra Min-Li Tan; Jo Keeble

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Lai Guan Ng

Singapore Immunology Network

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Suet-Mien Tan

Nanyang Technological University

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