Shunya Egawa

Gene expression in the vascular wall of the aortic arch in spontaneously hypertensive hyperlipidemic model rats using DNA microarray analysis

AIMS In recent years, there has been an increase in patients with arteriosclerosis and the risk of lifestyle-related diseases. However, the pathogenesis and medication of atherosclerosis have not been elucidated. We developed a rat model of lifestyle-related diseases by feeding a high-fat diet and 30% sucrose solution (HFDS) to spontaneously hypertensive hyperlipidemic rats (SHHR) and reported that this model is a useful model of early atherosclerosis. In order to elucidate the pathogenesis of early atherosclerosis, we searched for atherosclerosis-related genes by microarray analysis using the aortic arch rat model of lifestyle-related diseases. MAIN METHODS Four-month-old male Sprague-Dawley rats and SHHR were each divided into two normal diet (ND) groups and two HFDS groups. After a four-month treatment, the expression of mRNA in the aortic arch was detected using the oligo DNA microarray one-color method and quantified using real-time PCR. KEY FINDINGS In this study, we detected 39 genes in microarray analysis. Esm1, Retnlb Mkks, and Grem2 showed particularly marked changes in gene expression in the SHHR-HFDS group. Compared with the SD-ND group, the SHHR-HFDS group had an increase in Mkks gene expression of about 26-fold and an approximately 22-fold increase in the expression of Grem2. Similarly, the expression of Esm1 increased by about 12-fold and that of Retnlg by about 10-fold as shown by quantitative real-time PCR. SIGNIFICANCE This study suggested that these four genes might be important in early atherosclerosis development.

A case of metastasis to the thyroid gland from lung carcinoma

A case of metastasis to the thyroid gland from lung carcinoma: Toshimitsu Komatsuzaki1), Shunya Egawa1), Ken-ichiro Ikeda1,2), Yukiomi Kushihashi1,2), Yoichi Ikenoya1), Taisuke Hamasaki1), Suguru Furukawa1), Tomomi Mizuyoshi1), Masayo Asano1), Hitome Kobayashi1) and Toshikazu Shimane2,3). 1)Department of Otorhinolaryngology, School of Medicine, Showa University, 2) Head and Neck Oncology Center, School of Medicine, Showa University, 3)Department of Oral Surgery, School of Dentistry, Showa University