Silvana Maris Cirio

MEMBRANA BIOLÓGICA (Biofill ) - ESTUDO COMPARATIVO COM OUTROS AGENTES PROMOTORES DA CICATRIZAÇÃO DA PELE EM SUÍNOS: ASPECTOS CLÍNICOS, HISTOPATOLÓGICOS E MORFOMÉTRICOS

Com o objetivo de comparar a acao de diferentes agentes terapeuticos promotores da cicatrizacao da pele, foi realizado um estudo clinico, histopatologico e morfometrico em suino. Utilizou-se seis animais mesticos provenientes de uma mesma leitegada com 90 dias de idade. Foram produzidas seis lesoes experimentais quadrangulares do mesmo tamanho na regiao dorso-lombar e uma delas permaneceu como controle. Em cada animal, foram testados cinco tratamentos: Biofill humano, Biofill veterinario, Tegaderm, Duoderm e Nitrofurazona. Foram realizadas biopsias aos 7, 14, 21, 45 e 60 dias da evolucao cicatricial, seguidas de analise histopatologica. No periodo de 7 a 45 dias, realizou-se morfometria - perimetro e superficie inscrita - dos leitos cicatriciais. Foram as seguintes as membranas, pela ordem de eficiencia, nao so quanto a cicatrizacao mas tambem com relacao a adesao ao leito da ferida: Biofill Humano, Biofill Veterinario, Tegaderm e Duoderm. A Nitrofurazona comportou-se, em relacao ao aspecto cicatricial, de uma forma muito proxima a do Biofill; contanto, a necessidade de trocas diarias de curativos tornou o tratamento dificil. Abstract In order to compare different skin healing promoters, it was conducted a clinical, histopathological and morphometric study in swine. Six cross bred 90 days old animals from the same parturition were employed. Six experimental quadrangular wounds were made over the back region of each animal, one of them remaing as control. Five treatments were tested: Human Biofill, Veterinary Biofill, Tegaderm, Duoderm and Nitrofurazone At days 7, 14, 21, 45 and 60, biopsies for histopathological analysis were carried out. From de 7th to the 45th day, a morphometric study (perimeter and area) of the healing wounds beds was achieved. With the criteria of healing quality and adhesion to the wound the occlusive dressings in order of efficiency were: Human Biofill, Veterinary Biofill, Tegaderm and Duoderm. Nitrofurazone showed good healing qualities needing however daily changes of dressings.

Nonsyndromic Cleft Lip and Palate in Boxer Dogs: Evidence of Monogenic Autosomal Recessive Inheritance

Four newborn boxer dogs with bilateral cleft lip and palate were examined in search of further abnormalities, but no other type of congenital defect was found. These data, along with the pedigree inspection regarding the clinical phenotype, led to the conclusion that the affected dogs had a nonsyndromic monogenic autosomal recessive cleft lip and palate.

VACTERL association in a cat

We describe a female domestic cat with apparent VATER/VACTERL association, including vertebral abnormalities, anal atresia, radial agenesis, and cardiovascular and renal defects. If we consider the acronym VATER, this cat had a triad (VAR); however, if we consider the extended acronym VACTERL, she had a pentad (VACRL).

Infection in domestic dogs (Canis lupus familiaris, Linnaeus, 1758) from Guaratuba, Paraná, Brazil by circulating microfilariae of Acanthocheilonema reconditum (Grassi, 1889).

Os quadros epidemiologicos das zoonoses periodicamente sofrem modificacoes em alguns de seus aspectos, envolvendo a emergencia ou reemergencia de agravos a saude do homem e dos animais. Neste cenario sujeito a rapidas transformacoes, a analise da prevalencia de doencas, a exemplo das filarioses diagnosticadas em caes no litoral do Estado do Parana, apresenta significada relevância. O presente estudo foi realizado com 422 caes e teve como objetivo determinar a prevalencia de microfilarias circulantes de Acanthocheilonema reconditum tanto nos animais alojados no canil municipal, como naqueles domiciliados no bairro COHAPAR II, ambos localizados na regiao metropolitana da cidade de Guaratuba–Parana, atraves de dois exames hematologicos: avaliacao do sangue a fresco pela tecnica da gota espessa e metodo de Knott modificado por Newton e Wright. Trinta dos animais avaliados (7,10%), 22 machos e oito femeas, foram positivos para microfilarias de A. reconditum .

Immediate postoperative evaluation of enteric anastomosis after the use of meloxicam in rats

PURPOSE To compare ileal anastomoses in the immediate postoperative healing period after meloxicam use. METHODS Forty two male Wistar rats were randomly divided into two groups of 21, COX and control group. To COX meloxicam in combination with morphine was given in 3 days period. Control group received only morphine during the same period. Each group was divided into three sub-groups of 7, which were euthanized at 5, 10, and 21 days postoperatively. Comparison was based in histological evaluation of collagen type I and III using sirius red, immunohistochemical through vascular endothelial growth factor and matrix metalloproteinase-9. RESULTS Healing process in scheduled periods did not show significant differences (p>0.05) between the COX and control groups during any of the periods. CONCLUSION The use of meloxicam in the postoperative period following ileal anastomosis did not affect healing.

Pierre Robin Sequence: A Familial, Clinical, and Pathoanatomical Record of an Affected Dachshund

This study describes a spontaneous case of Pierre Robin sequence in a nonhuman animal species. A miniature dachshund with micrognathia developed glossoptosis, respiratory distress, dysphagia, temporomandibular ankylosis, and a misaligned upper jaw. The severity of this condition resulted in death by obstructive apnea at the age of 8 mo. Dogs with Pierre Robin sequence can provide further knowledge and a greater understanding of this abnormality, leading to better management of affected individuals and improvement of therapeutic methods.

Follicular dysplasia of the adult doberman pinscher.

This paper presents the case of an adult female, red Doberman pinscher affected by permanent hypotrichosis, limited to the dorsolumbar region and sides of the trunk. The hypotrichosis began at approximately the age of 2 years and progressed slowly with no skin hyperpigmentation. The clinical and histopathological characteristics are of an uncommon form of follicular dysplasia.

LIGNEOUS CONJUNCTIVITIS IN A HORSE: A CASE OF ELEVEN MONTHS DURATION

A six months old throughbred female race horse was received presenting events of mucous purulent discharge from the right eye for two days. Close examination of both eyes revealed bilateral mucous purulent conjunctivitis, more severe on the left eye where discharge was more profuse, with tarsal and bulbar chemosis and blepharospasm. This animal also had a fluoresceine fixing superficial linear ulcer on the left eye. It was performed culture of material from the botton of conjunctive sack, which revealed an infection by Pseudomonas spp, sensitive to tobramycine. Based upon microbiological tests, tobramycine eye drops (15 mg/ml) and ophtalmic ointment (15 mg/g) was given for three weeks, and a two percent atropine and one percent dichorphenac eye drops were given for five days. At the end of the treatment, the right eye was calm and without alterations while the left eye displayed a tarsal membranous conjunctivitis with minimal serous ocular discharge. The presence of an allergic conjunctivitis was suspected and four percent dissodium chromoglicate eye drops were given. In spite of the decrease of inflamation observed, the conjunctive membranes remained. Surgical debridement of the membranes was performed and representative material was collected and sent for histological examination. Based upon human and canine reports and histological findings, the most probable diagnosis was ligneous conjunctivitis. After biopsy, the drug chromoglicate was replaced by one percent prednisolone eye drops for three weeks. After this, the eye showed a discreet dorsal tarsal chemosis and hyperemia but no membranous conjunctivitis. As supporting therapy a daily morning instillation of corticoid for 30 days was ordered. Five months later, a recurrence of the membranous conjunctivitis was verified and these membranes invaded the cornea, causing temporal pigmentary keratitis. A two percent cyclosporine ointment was then given and a new biopsy asked. The diagnosis of recurrent severe ligneous conjunctivitis was evident, and a wide surgical resection of the membranes and vascular cauterization was decided. Postoperative treatment was composed by one percent prednisolone, one and a half percent tobramicine eye drops together with 5,000 IU /ml heparin. Treatment proceeded for 30 days when the antibiotic was taken off, corticoid frequency was reduced and topic heparin kept. Ligneous conjunctivitis is a chronic membranous conjunctivitis with presence of membranes mainly in the tarsal superior conjunctives, of ignored etiology. Up to the present, two months after the surgical resection of the membranes, the topical use of corticoid and heparin satisfactorily controls the case, now eleven months old.