Soichiro Yamamura

Fluorescent fibre-optic immunosensing system based on complement lysis of liposome containing carboxyfluorescein

A novel type of fibre-optic immunosensing system has been developed. Agar gel-immobilized liposomes containing carboxyfluorescein were attached to the tip of an optical fibre. Complement-mediated immunolysis of the liposome was fluorometrically detected through the fibre. By using dinitrophenyl (DNP) hapten-loaded liposomes, concentrations as low as 500-fold diluted anti-DNP antibody and 0.76 CH50 ml-1 of complement were detected.

Photoelectrochemical reduction of carbon monoxide using p-Si

Donnees sur la reduction photoelectrochimique du monoxyde de carbone, en solution aqueuse, sous irradiation par de la lumiere visible, avec formation de formaldehyde. Donnees numeriques

Time-resolved luminescence studies of a ruthenium(II) complex on porous glass. Pore size dependence of photoinduced electron transfer

Abstract Photoinduced electron transfer occurs from Ru(bpy) 3 2+ (bpy, bipyridine) adsorbed on porous glass to aqueous titanium species in the pore. The lifetime of excited Ru(bpy) 3 2+ decreases with an increase in the pore size, indicating that the lifetime reflects the mobility of the molecules in the pore.

Fluorimetry of haemolyis of red blood cells by catalytic reaction of leaked haemoglobin: Application to homoegeneous fluorescence immunoassay

A novel method was developed for the homogeneous fluorescence immunoassay using complement-mediated haemolysis of sheep red blood cells. Fluorescent substrates of peroxidase, 4-hydroxyphenylpropionic acid (HPPA) or fluorescin were catalysed by haemoglobins leaked from haemolysed red blood cells. Non-haemolysed cells showed no enzymatic activity and only haemoglobins released from the cells showed catalytic activity. The extent of hemolysis could therefore be measured without separating haemolysed and non-haemolysed cells. The immunological binding of antibodies with the antigens on the cell membrane activates the complement system and the cell is finally lysed. Homogeneous fluorescence immunoassay was performed by combining the complement-mediated haemolysis technique and the fluorimetry of haemolysis. The immunoassay was done for the immunoagents; complement and bovine serum alubmin (BSA). The sensitivity was 0.051 CH50 unit ml−1for complement and < 1.0 μg ml−1 of BSA.

Photographic homogeneous immunoassay based on luminol chemiluminescence catalyzed by hemoglobins leaked from complement-hemolyzed red blood cells

SummaryHuman serum albumin (HSA) and complement were photographically assayed. The complement activated by the formed antigen-antibody complex causes the hemolysis of red blood cells. Leaked hemoglobins catalyze the chemiluminescent reaction of luminol and hydrogen peroxide (H2O2). The resulting emission was recorded on instant film.