Søren Laurentius Nielsen

Depth Colonization of Eelgrass (Zostera marina) and Macroalgae as Determined by Water Transparency in Danish Coastal Waters

We present a comparative analysis of lower depth limits for growth of eelgrass, large brown algae and other macroalgae measured by SCUBA-diving along 162 transects in 27 Danish fjords and coastal waters, coupled to 1,400 data series of water chemistry (especially nitrogen) and Secchi depth transparency collected between March and October. Danish coastal waters are heavily eutrophied and characterized by high particle concentrations, turbid water and lack of macrophyte growth in deep water. Median values are 3.6 m for Secchi depth and median lower-depth limits are 4.0 m for eelgrass, 5.3 m for brown algae and 5.0 m for other macroalgae. Depth limits for growth of eelgrass and macroalgae increase linearly with transparency in the coastal waters. The relationships are highly significant (p<10−6) and transparency accounts for about 60% of the variability of depth limits. Eelgrass extends approximately to half the maximum depth of macroalgae, presumably because of greater respiratory costs to maintain the below-ground rhizomes and roots of eelgrass, which often constitutes half the plant weight. As a reflection of the importance of total nitrogen (TN) in controlling phytoplankton biomass and thus Secchi depth in coastal marine waters, we found that TN could explain 48–73% of the variation in depth limits of eelgrass and macroalgae, according to a multiplicative model (Y=aXb). As with Secchi depth, the relationship to eelgrass showed a lower slope, reflecting the higher respiratory costs of eelgrass. The models show great sensitivity and a profound quantitative response with proportional effects on Secchi depth and depth limits when total-N concentrations are reduced.

Estuarine nutrient cycling: The influence of primary producers

Interactions Between Vegetation And Nutrient Dynamics In Coastal Marine Ecosystems: An Introduction by M.F. Pedersen, S.L. Nielsen and G.T. Banta Estuarine Primary Producers by K. Sand-Jensen and S.L. Nielsen Effects Of Nutrient Loading On Shallow Seagrass-Dominated Coastal Systems: Patterns And Processes by J. Hauxwell and I. Valiela Plant Bound Nutrient Transport. Mass Transport In Estuaries And Lagoons by M.R. Flindt, J. Neto, C.L. Amos, M.A. Pardal, A. Bergamasco, C.B. Pedersen and F.O. Andersen Grazing On Pelagic Primary Producers - The Role Of Benthic Suspension Feeders In Estuaries by J.K. Petersen Grazing On Benthic Primary Producers by J. Cebrian Decomposition Of Marine Primary Producers: Consequences For Nutrient Recycling And Retention In Coastal Ecosystems by G.T. Banta, M.F. Pedersen and S.L. Nielsen Burial Of Nutrient In Coastal Sediments: The Role Of Primary Producers by J.J. Middelburg, K. Soetaert, P.M.J. Herman, H.T.S. Boschker and C.R. Heip The Importance Of Primary Producers For Benthic Nitrogen And Phosphorus Cycling by K.J. McGlathery, K. Sundback and I.C. Anderson Denitrification by N. Risgaard-Petersen Attempting A Synthesis - Plant/Nutrient Interactions by S.L. Nielsen, M.F. Pedersen and G.T. Banta

Phytoplankton, nutrients, and transparency in Danish coastal waters

We present a comparative analysis of 1400 data series of water chemistry (particularly nitrogen and phosphorus concentrations), phytoplankton biomass as chlorophylla (chla) concentrations, concentrations of suspended matter and Secchi depth transparency collected from the mid-1980s to the mid-1990s from 162 stations in 27 Danish fjords and coastal waters. The results demonstrate that Danish coastal waters were heavily eutrophied and had high particle concentrations and turbid waters. Median values were 5.1 μg chla 1−1, 10.0 mg DW 1−1 of suspended particles, and Secchi depth of 3.6 m. Chlorophyll concentration was strongly linked to the total-nitrogen concentration. The strength of this relationship increased from spring to summer as the concentration of total nitrogen declined. During summer, total nitrogen concentrations accounted for about 60% of the variability in chlorophyll concentrations among the different coastal systems. The relationship between chlorophyll and total phosphorus was more consistant over the year and correlations were much weaker than encountered for total nitrogen. Secchi depth could be predicted with good precision from measurements of chlorophyll and suspended matter. In a multiple stepwise regression model with In-transformed values the two variables accounted for most of the variability in water transparency for the different seasons and the period March–October as a whole (c. 80%). We were able to demonstrate a significant relationship between total nitrogen and Secchi depth, with important implications for management purposes.

Distribution, structure and function of Nordic eelgrass (Zostera marina) ecosystems: implications for coastal management and conservation

This paper focuses on the marine foundation eelgrass species, Zostera marina, along a gradient from the northern Baltic Sea to the north-east Atlantic. This vast region supports a minimum of 1480 km2 eelgrass (maximum >2100 km2), which corresponds to more than four times the previously quantified area of eelgrass in Western Europe. Eelgrass meadows in the low salinity Baltic Sea support the highest diversity (4–6 spp.) of angiosperms overall, but eelgrass productivity is low (<2 g dw m-2 d-1) and meadows are isolated and genetically impoverished. Higher salinity areas support monospecific meadows, with higher productivity (3–10 g dw m-2 d-1) and greater genetic connectivity. The salinity gradient further imposes functional differences in biodiversity and food webs, in particular a decline in number, but increase in biomass of mesograzers in the Baltic. Significant declines in eelgrass depth limits and areal cover are documented, particularly in regions experiencing high human pressure. The failure of eelgrass to re-establish itself in affected areas, despite nutrient reductions and improved water quality, signals complex recovery trajectories and calls for much greater conservation effort to protect existing meadows. The knowledge base for Nordic eelgrass meadows is broad and sufficient to establish monitoring objectives across nine national borders. Nevertheless, ensuring awareness of their vulnerability remains challenging. Given the areal extent of Nordic eelgrass systems and the ecosystem services they provide, it is crucial to further develop incentives for protecting them.

Distribution, structure and function of Nordic eelgrass (Zostera marina) ecosystems

This paper focuses on the marine foundation eelgrass species, Zostera marina, along a gradient from the northern Baltic Sea to the north-east Atlantic. This vast region supports a minimum of 1480 km2 eelgrass (maximum >2100 km2), which corresponds to more than four times the previously quantified area of eelgrass in Western Europe. Eelgrass meadows in the low salinity Baltic Sea support the highest diversity (4–6 spp.) of angiosperms overall, but eelgrass productivity is low (<2 g dw m-2 d-1) and meadows are isolated and genetically impoverished. Higher salinity areas support monospecific meadows, with higher productivity (3–10 g dw m-2 d-1) and greater genetic connectivity. The salinity gradient further imposes functional differences in biodiversity and food webs, in particular a decline in number, but increase in biomass of mesograzers in the Baltic. Significant declines in eelgrass depth limits and areal cover are documented, particularly in regions experiencing high human pressure. The failure of eelgrass to re-establish itself in affected areas, despite nutrient reductions and improved water quality, signals complex recovery trajectories and calls for much greater conservation effort to protect existing meadows. The knowledge base for Nordic eelgrass meadows is broad and sufficient to establish monitoring objectives across nine national borders. Nevertheless, ensuring awareness of their vulnerability remains challenging. Given the areal extent of Nordic eelgrass systems and the ecosystem services they provide, it is crucial to further develop incentives for protecting them.

Temperature acclimation and heat tolerance of photosynthesis in Norwegian Saccharina latissima (Laminariales, Phaeophyceae)

Kelps, seaweeds and seagrasses provide important ecosystem services in coastal areas, and loss of these macrophytes is a global concern. Recent surveys have documented severe declines in populations of the dominant kelp species, Saccharina latissima, along the south coast of Norway. S. latissima is a cold‐temperate species, and increasing seawater temperature has been suggested as one of the major causes of the decline. Several studies have shown that S. latissima can acclimate to a wide range of temperatures. However, local adaptations may render the extrapolation of existing results inappropriate. We investigated the potential for thermal acclimation and heat tolerance in S. latissima collected from three locations along the south coast of Norway. Plants were kept in laboratory cultures at three different growth temperatures (10, 15, and 20°C) for 4–6 weeks, after which their photosynthetic performance, fluorescence parameters, and pigment concentrations were measured. S. latissima obtained almost identical photosynthetic characteristics when grown at 10 and 15°C, indicating thermal acclimation at these temperatures. In contrast, plants grown at 20°C suffered substantial tissue deterioration, and showed reduced net photosynthetic capacity caused by a combination of elevated respiration and reduced gross photosynthesis due to lowered pigment concentrations, altered pigment composition, and reduced functionality of Photo‐system II. Our results support the hypothesis that extraordinarily high temperatures, as observed in 1997, 2002, and 2006, may have initiated the declines in S. latissima populations along the south coast of Norway. However, observations of high mortality in years with low summer temperatures suggest that reduced population resilience or other factors may have contributed to the losses.

Growth rates and morphological adaptations of aquatic and terrestrial forms of amphibious Littorella uniflora (L.) Aschers.

Morphological – anatomical features of the terrestrial and the aquatic life form of the rosette species Littorella uniflora, inhabiting nutrient poor soils of oligotrophic lakes, were investigated together with growth rates of both life forms and of transplants. Growth rates were the same for the two life forms. However, growth of transplanted plants was somewhat reduced by transition from one environment to another. This was especially true for aquatic plants, which may be stressed by desiccation when moved to the terrestrial environment. The morphological – anatomical differences between the life forms were small compared with many other amphibious species which produce highly specialized leaves and life forms in air and under water. It is suggested that the conservative leaf morphology of Littorella is a consequence of the high dependence on rhizospheric CO2 of both the aquatic and the terrestrial form of Littorella, making production of leaves specialized for carbon uptake in one specific environment unnecessary.

Apparent lack of vesicular–arbuscular mycorrhiza (VAM) in the seagrasses Zostera marina L. and Thalassia testudinum Banks ex König

Abstract We examined two populations of Zostera marina L. and one of Thalassia testudinum Banks ex Konig for the presence of vesicular–arbuscular mycorrhiza (VAM). None of these plants showed any VAM colonization. In addition, we were unable to find any literature references on the presence of VAM in seagrasses, although VAM has been found in a wide variety of submerged and emergent freshwater angiosperms as well as in salt marsh plants. If the lack of data on VAM in seagrasses in the literature can be attributed to an absence of VAM in seagrasses, it can be hypothesized to be due to the effect of low oxygen levels in marine sediments and high salinities as joint stressors. These factors have been found to reduce VAM colonization in other plant types.

Growth, photosynthesis and nutrient content of seedlings and mature plants of Cymodocea nodosa — the importance of clonal integration

Abstract This paper presents data on nutrient (N and P) content, chlorophyll content, dark respiration, photosynthetic rate and relative growth rate of four types of shoots of Cymodocea nodosa in Alfacs Bay, NE Spain: (1) seedlings, i.e. young shoots still attached to the seed coat, (2) shoots in runners, i.e. shoots growing in fast-growing horizontal rhizomes extending centrifugally from the patch edge, (3) mature shoots with well-developed rhizomes growing in the patch proper, and (4) isolated mature shoots not attached to a seed coat and with rhizomes, but growing singly or with only a few (

Adaptation to high light irradiances enhances the photosynthetic Cu2+ resistance in Cu2+ tolerant and non-tolerant populations of the brown macroalgae Fucus serratus.

The relationship between light acclimation and Cu(2+) tolerance was studied in two populations of Fucus serratus known to be naturally non-tolerant and tolerant to Cu(2+). Acclimation to high irradiances increased the photosynthetic tolerance to Cu(2+). The xanthophyll cycle was apparently not involved in protecting the photosynthetic apparatus against Cu(2+) toxicity, as results showed that Cu(2+) did not induce dynamic photoinhibition. The higher photosynthetic Cu(2+) resistance of high light algae did not result in increased growth. The excess energy acquired by high light-adapted algae appeared to be utilized in Cu(2+) defense mechanisms in the Cu(2+) non-tolerant population. The polyphenol content of the algae was reciprocal to the Cu(T) content, suggesting that polyphenol may be the primary Cu(2+) defense of non-tolerant low light algae, acting through secretion and extracellular chelating of Cu(2+), while the compounds do not seem to be involved in the primary Cu(2+) tolerance mechanism in Cu(2+) tolerant algae.