Stacey Roberts

Ideal Ratios of Isoleucine, Methionine, Methionine Plus Cystine, Threonine, Tryptophan, and Valine Relative to Lysine for White Leghorn-Type Laying Hens of Twenty-Eight to Thirty-Four Weeks of Age

Seven separate experiments were conducted with Hy-Line W-36 hens to determine the ideal ratio of Arg, Ile, Met, Met+Cys, Thr, Trp, and Val relative to Lys for maximal egg mass. The experiments were conducted simultaneously and were each designed as a randomized complete block design with 60 experimental units (each consisting of 1 cage with 2 hens) and 5 dietary treatments. The 35 assay diets were made from a common basal diet (2,987 kcal/kg of ME; 12.3% CP; 4.06% Ca, 0.47% nonphytate P), formulated using corn, soybean meal, and meat and bone meal. The true digestible amino acid contents in the basal diet were determined using the precision-fed assay with adult cecectomized roosters. Crystalline L-Arg (free base), L-Ile, L-Lys.HCl, DL-Met, L-Thr, L-Trp, and L-Val (considered 100% true digestible) were added to the basal diet at the expense of cornstarch to make the respective assayed amino acid first limiting and to yield 5 graded inclusions of the assayed amino acid. Hens were fed the assay diets from 26 to 34 wk of age, with the first 2 wk considered a depletion period. Egg production was recorded daily and egg weight was determined weekly on eggs collected over 48 h; egg mass was calculated as egg production x egg weight. The requirement for each amino acid was determined using the broken-line regression method. Consumption of Arg did not affect egg mass, thus a requirement could not be determined. The true digestible amino acid requirements used to calculate the ideal amino acid ratio for maximum egg mass were 426 mg/d of Ile, 538 mg/d of Lys, 253 mg/d of Met, 506 mg/d of Met+Cys, 414 mg/d of Thr, 120 mg/d of Trp, and 501 mg/d of Val. The ideal amino acid ratio for maximum egg mass was Ile 79%, Met 47%, Met+Cys 94%, Thr 77%, Trp 22%, and Val 93% on a true digestible basis relative to Lys. The ideal Met and Met+Cys ratios were verified in an ensuing identical experiment with 52- to 58-wk-old hens.

Reducing ammonia emissions from laying-hen houses through dietary manipulation.

Feed additives can change the microbiological environment of the animal digestive track, nutrient composition of feces, and its gaseous emissions. This 2-yr field study involving commercial laying-hen houses in central Iowa was conducted to assess the effects of feeding diets containing EcoCal and corn-dried distillers grain with solubles (DDGS) on ammonia (NH3), hydrogen sulfide (H2S), and greenhouse gas (CO2, CH4, and N2O) emissions. Three high-rise layer houses (256,600 W-36 hens per house) received standard industry diet (Control), a diet containing 7% EcoCal (EcoCal) or a diet containing 10% DDGS (DDGS). Gaseous emissions were continuously monitored during the period of December 2007 to December 2009, covering the full production cycle. The 24-month test results revealed that mean NH3 emission rates were 0.58 ± 0.05, 0.82 ± 0.04, and 0.96 ± 0.05 g/hen/day for the EcoCal, DDGS, and Control diet, respectively. Namely, compared to the Control diet, the EcoCal and DDGS diets reduced NH3 emission by an average of 39.2% and 14.3%, respectively. The concurrent H2S emission rates were 5.39 ± 0.46, 1.91 ± 0.13, and 1.79 ± 0.16 mg/hen/day for the EcoCal, DDGS, and Control diet, respectively. CO2 emission rates were similar for the three diets, 87.3 ± 1.37, 87.4 ± 1.26, and 89.6 ± 1.6 g/hen/day for EcoCal, DDGS, and Control, respectively (P = 0.45). The DDGS and EcoCal houses tended to emit less CH4 than the Control house (0.16 and 0.12 vs. 0.20 g/hen/day) during the monitored summer season. The efficacy of NH3 emission reduction by the EcoCal diet decreased with increasing outside temperature, varying from 72.2% in February 2009 to −7.10% in September 2008. Manure of the EcoCal diet contained 68% higher ammonia nitrogen (NH3-N) and 4.7 times higher sulfur content than that of the Control diet. Manure pH values were 8.0, 8.9, and 9.3 for EcoCal, DDGS, and Control diets, respectively. This extensive field study verifies that dietary manipulation provides a viable means to reduce NH3 emissions from modern laying-hen houses. Implications This work demonstrated that dietary manipulation can be used to reduce NH3 emissions from high-rise laying-hen houses with no adverse effect on the hen production performances (to be presented separately). The NH3 reduction rates could vary with different climates and hence geographic locations. The dietary manipulation to lower NH3 emissions should be applicable to all egg production systems. The results of this study also contribute to the baseline data for improving the national air emissions inventory for livestock and poultry production facilities.

Evaluation of a Flux Chamber for Assessing Gaseous Emissions and Treatment Effects of Poultry Manure

The need to quantify air emissions from animal feeding operations (AFOs) with relative ease and reasonable certainty continues to rise. Exploration of practical means to reduce air emissions also calls for less sophisticated but reasonably dependable methods to quantify the treatment effect. Although mobile air emissions monitoring units (MAEMUs) capable of precise and real-time emission measurement is the norm for continuous, intensive monitoring of emissions from mechanically ventilated animal facilities, their relative immobility and high cost are limiting the widespread use. Several other methods, such as gas-washing, micro-meteorological, wind tunnel, flux chamber, and mass-balance methods, have been employed to accommodate different measurement needs. Flux chambers have the advantages of being portable, small size, low cost, and less labor requirement. The objectives of this study were: (1) to develop a portable emission flux chamber system (EFC) for in-situ measurement of ammonia (NH3) and carbon dioxide (CO2) emissions from manure; (2) to assess gaseous (NH3 and CO2) emissions of high-rise layer houses with the EFC vs. MAEMU; and (3) to evaluate the adequacy of using the EFC to determine the effects of dietary regimens on ammonia emissions from the layer manure. The preliminary data showed that NH3 emission from the manure surface measured with the EFC was 8% to 16% that of the whole barn measured with the MAEMU, while CO2 emission from the manure surface was 1% to 4% of the barn emission. The preliminary results obtained with EFC concerning the dietary efficacy of ammonia emission reduction were mixed as compared to those obtained with the MAEMU. More evaluation is continuing.

Effects of dietary modification on laying hens in high-rise houses: Part I - Emissions of ammonia, hydrogen sulfide and carbon dioxide.

Dietary manipulation can substantially lower ammonia (NH3) emissions from laying-hen houses or manure storage. Recent lab studies showed a reduction of 40–60% in ammonia emissions for an experimental (EcoCalTM) diet as compared the standard or control diet. However, adoption of a mitigation technology at commercial production level should be preceded by substantial field verification tests to document not only NH3 emission reduction, but also impact of the strategy on production performance of the hens and cash returns. A study to assess the effects of feeding diets containing EcoCal on NH3, hydrogen sulfide (H2S), and carbon dioxide (CO2) emissions, laying-hen production performance, and economic returns was conducted at a commercial laying-hen farm in central Iowa. Two houses (256,000 or 262,000 hens per house) were used for the study. Hens in one house were fed the EcoCal diet while hens in the other house were fed a standard or control diet containing no EcoCal. A state-of-the-art mobile air emissions monitoring unit (MAEMU) and the associated sampling system were used to continuously monitor the gaseous concentrations, ventilation rate and environmental conditions. Comparative data collected from December 2006 to May 2007 are presented in this paper. Data from this period showed that the EcoCal diet led to NH3 emission reduction by up to 23.2% (0.86±0.04 and 1.12±0.03 g/d·hen for EcoCal and Control diet, respectively), at the same time, H2S emission increased by up to 134% (4.38±0.20 and 1.82±0.07 mg/d·hen for EcoCal and Control diet, respectively), although the magnitude of H2S emission is rather small for both dietary regimens. Data on the hen production performance are reported in a companion paper (Roberts et al., 2008).

Effects of Laying-Hen Strain on Manure Properties and Ammonia Emission

Ammonia (NH3) emissions from laying hens are affected by nutrient content of the diet, manure quantity, and manure properties such as moisture content, nitrogen content, and pH. These production traits may vary with strain of the hen. However, limited information is available concerning the effects of laying-hen genetics on manure properties and NH3 emission. This study was conducted to comparatively quantify production performance, manure properties, and NH3 emissions (through N mass balance) of four white-egg-laying strains (Hy-Line W-36, Hy-Line W-98, Lohmann LSL Lite, and Bovans White) and four brown-egg-laying strains (Hy-Line Brown, Lohmann Brown, ISA Brown, and Bovans Brown) during two production periods of 27-28 weeks (P1) and 35-36 weeks (P2) of age. The diets were formulated to meet the nutritional needs of the brown and white hens. As a result, crude protein contents during P1 and P2 were, respectively, 13.2% and 15.2% for the brown hens but 14.5% and 17.4% for the white hens. The results showed that the brown and white hens had similar hen-day egg production (97.5% to 89.2% for brown hens and 96.0% to 88.2% for white hens) and egg mass output (57.1 to 52.6 g d-1 hen-1 for brown hens and 55.6 to 51.2 g d-1 hen-1 for white hens) but different feed consumption (112 to 98 g d-1 hen-1 for brown hens and 101 to 93 g d-1 hen-1 for white hens, p < 0.01) and feed efficiency (1.97 to 1.87 g feed g-1 egg for brown hens and 1.82 g feed g-1 egg for white hens, p < 0.0001 and p = 0.11). The higher feed consumption for the brown hens stemmed from their heavier body mass (1.81 to 1.78 kg vs. 1.56 to 1.53 kg for white hens). Manure moisture content was higher for the brown hens than for the white hens, although the dry-matter manure production was not significantly different. The results further revealed that under the experimental conditions (i.e., higher CP contents of the diet for the white hens than for the brown hens) the white hens had higher NH3 emissions than the brown hens as expressed per hen (37% to 19% higher, p = <0.001 to 0.016), per animal unit (AU, 500 kg live body mass; 59% to 39% higher, p = 0.0007 to 0.007), per unit of egg mass output (41% to 24% higher, p = 0.01 to 0.09), per unit of feed N consumed (39% to 27% higher, p = 0.01 to <0.0001), and per unit of dry manure (56% to 39% higher, p = 0.001 to 0.007). Certain differences existed in production performance among strains within the brown or white hens, but no differences in NH3 emissions were detected. Because of the relatively small sample size (number of hens involved) and the relatively short monitoring period, the results should be referenced with these limitations in mind. Further larger-scale studies with longer monitoring periods to verify these findings are warranted.