Stefan Jenewein
Hoffmann-La Roche
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Publication
Featured researches published by Stefan Jenewein.
Blood | 2011
Gerhard Niederfellner; Alfred Lammens; Olaf Mundigl; Guy Georges; Wolfgang Schaefer; Manfred Schwaiger; Andreas G. Franke; Kornelius Wiechmann; Stefan Jenewein; Jerry W. Slootstra; Peter Timmerman; Annika Brännström; Frida Lindstrom; Ekkehard Mössner; Pablo Umana; Karl-Peter Hopfner; Christian Klein
CD20 is a cell-surface marker of normal and malignant B cells. Rituximab, a monoclonal antibody targeting CD20, has improved the treatment of malignant lymphomas. Therapeutic CD20 antibodies are classified as either type I or II based on different mechanisms of killing malignant B cells. To reveal the molecular basis of this distinction, we fine-mapped the epitopes recognized by both types. We also determined the first X-ray structure of a type II antibody by crystallizing the obinutuzumab (GA101) Fab fragment alone and in complex with a CD20 cyclopeptide. Despite recognizing an overlapping epitope, GA101 binds CD20 in a completely different orientation than type I antibodies. Moreover, the elbow angle of GA101 is almost 30° wider than in type I antibodies, potentially resulting in different spatial arrangements of 2 CD20 molecules bound to a single GA101 or rituximab molecule. Using protein tomography, different CD20 complexes were found to be associated with the 2 antibodies, and confocal microscopy showed different membrane compartmentalization of these subpopulations of the cellular CD20 pool. Our findings offer a possible molecular explanation for the different cellular responses elicited by type I and II antibodies.
Archive | 2012
Monika Baehner; Stefan Jenewein; Manfred Kubbies; Ekkehard Moessner; Tilman Schlothauer
Blood | 2009
Gerhard Niederfellner; Alfred Lammens; Manfred Schwaiger; Guy Georges; Kornelius Wiechmann; Andreas G. Franke; Wolfgang Schaefer; Stefan Jenewein; Jerry W. Slootstra; Ekkehard Moessner; Pablo Umana; Karl-Peter Hopfner; Christian Klein
Archive | 2012
Stefan Jenewein; Stefan Klostermann; Erhard Kopetzki; Stefan Lorenz; Joerg Thomas Regula; Georg Tiefenthaler
Archive | 2011
Johannes Auer; Harald Duerr; Guy Georges; Stefan Jenewein; Klaus Kaluza; Olaf Mundigl; Stefan Ries; Jan Olaf Stracke
Archive | 2011
Michael Brandt; Jens Fischer; Stefan Jenewein; Klaus Kaluza; Eileen Samy; Stefan Seeber
Archive | 2011
Stefan Jenewein; Erhard Kopetzki; Stefan Ries; Georg Tiefenthaler
Archive | 2013
Ekkehard Moessner; Manfred Kubbies; Tilman Schlothauer; Monika Baehner; Stefan Jenewein
Archive | 2012
Monika Baehner; Stefan Jenewein; Manfred Kubbies; Ekkehard Moessner; Tilman Schlothauer
Archive | 2012
Stefan Jenewein; Stefan Klostermann; Erhard Kopetzki; Stefan Lorenz; Joerg Thomas Regula; Georg Tiefenthaler