Stefano Fedeli
University of Florence
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Publication
Featured researches published by Stefano Fedeli.
Journal of Materials Chemistry B | 2016
Stefano Fedeli; Alberto Brandi; Lorenzo Venturini; Paola Chiarugi; Elisa Giannoni; Paolo Paoli; Denise Corti; Giuliano Giambastiani; Giulia Tuci; Stefano Cicchi
To demonstrate the potential of azido-substituted carbon nanotubes for application in nanomedicine, multiple-decorated oxidized multi-walled carbon nanotubes as drug delivery systems have been synthesized. These DDSs were able to carry doxorubicin inside breast MCF-7 cancer cell lines resulting in an enhanced cytotoxic effect with respect to the free drug. Decoration of the carbon nanotubes was accomplished through both covalent and non-covalent approaches: versatile click reactions and π-π interactions were exploited. To assess the internalization of the carbon nanotubes inside cells, decoration with a BODIPY fluorescent molecule was performed. Furthermore, the nanotubes were decorated with a biotin selector molecule to increase the uptake of the system by cancer cells. Comparative studies were performed on the complete drug delivery system to highlight its effect with respect to the free drug and the contribution of the selector in the internalization efficiency. Finally, preliminary in vivo tests were performed on MCF-7 inoculated mice. A net improvement in efficiency, concerning the minor growth of the tumors, has been found when using doxorubicin loaded on our drug delivery system with respect to free doxorubicin.
Biosensors and Bioelectronics | 2017
S. Lisi; Simona Scarano; Stefano Fedeli; E. Pascale; Stefano Cicchi; C. Ravelet; E. Peyrin; Maria Minunni
Interest on Tau protein is fast increasing in Alzheimers disease (AD) diagnosis. There is the urgent need of highly sensitive and specific diagnostic platforms for its quantification, also in combination with the other AD hallmarks. Up to now, SPR has been poorly exploited for tau detection by immunosensing, due to sensitivity limits at nanomolar level, whereas the clinical requirement is in the picomolar range. Molecular architectures built in a layer-by-layer fashion, biomolecules and nanostructures (metallic or not) may amplify the SPR signal and improve the limit of detection to the desired sensitivity. Mostly gold nanostructures are widely employed to this aim, but great interest is also emerging in Multi Walled Carbon Nanotubes (MWCNTs). Here MWCNTs are modified and then decorated with the secondary antibody for tau protein. Eventually we took advantage from MWCNTs-antibody conjugate to obtain a sandwich-based bioassay with the capability to increase the SPR signal of about 102 folds compared to direct detection and conventional unconjugated sandwich. With respect to these results, we hope to give a strong impulse for further investigation on studying possible roles of carbon nanotubes in optical-based biosensing.
Chemistry: A European Journal | 2015
Stefano Fedeli; Paolo Paoli; Alberto Brandi; Lorenzo Venturini; Giuliano Giambastiani; Giulia Tuci; Stefano Cicchi
A series of azido-dyes were synthesized through Knoevenagel reactions of an azido-BODIPY with aromatic aldehydes. The nature of the substituents allowed the fine tuning of their spectroscopic properties. The dyes were used to decorate oxidized multiwalled carbon nanotubes (ox-MWCNTs), bearing terminal triple bond groups, by CuAAC reactions, affording fluorescent materials. This decoration allowed the efficient determination of the internalization of the ox-MWCNT derivatives by different model cancer cells, such as MCF7.
International Journal of Pharmaceutics | 2017
Niccolò Sciortino; Stefano Fedeli; Paolo Paoli; Alberto Brandi; Paola Chiarugi; Mirko Severi; Stefano Cicchi
Batches of oxidized multiwalled carbon nanotubes differing in length were adopted to prepare two drug delivery systems (DDS) loaded with doxorubicin. The different internalization of the two batches, verified by atomic emission spectroscopy onto cell lysates, was also confirmed by the different toxicity of the same DDS loaded with doxorubicin. In vitro experiments evidenced, after 48h of incubation, the superior efficacy of the shortest nanotubes. However, upon prolonging the incubation time up to 72h the difference in efficiency was minimized due to the spontaneous release of doxorubicin by the non-internalized long nanotubes.
Journal of Materials Chemistry B | 2018
Giacomo Biagiotti; Stefano Fedeli; Giulia Tuci; Lapo Luconi; Giuliano Giambastiani; Alberto Brandi; Federica Pisaneschi; Stefano Cicchi; Paolo Paoli
The progress of the chemistry of carbon nanotubes (CNT) and graphene derivatives [mainly graphene oxide (GO)] has produced a number of technologically advanced drug delivery systems (DDS) that have been used in the field of nanomedicine, mostly in studies related to oncology. However, such a demanding field of research requires continuous improvements in terms of efficiency, selectivity and versatility. The loading of two, or more, bioactive components on the same nanoparticle offers new possibilities for treating cancer, efficiently addressing issues related both to biodistribution and pharmacokinetics. Nanostructured carbon materials (NCM), with their high surface area, their efficient cellular membrane crossing and their chemical versatility are ideal candidates for easy hetero-decoration and exploitation as advanced DDS. This review describes the achievements obtained in this area focusing on those studies in which two or more active components were loaded onto the DDS.
Progress in Heterocyclic Chemistry | 2014
Luisa Lascialfari; Stefano Fedeli; Stefano Cicchi
Abstract The functionalisation of fullerene, carbon nanotubes and graphene, to form heterocyclic derivatives is described, demonstrating the fundamental role of heterocyclic chemistry in the fast growing area of materials chemistry.
Journal of Physical Chemistry C | 2014
Mariangela Di Donato; Alessandro Iagatti; Andrea Lapini; Paolo Foggi; Stefano Cicchi; Luisa Lascialfari; Stefano Fedeli; Stefano Caprasecca; Benedetta Mennucci
Journal of Physical Chemistry C | 2016
Alessandro Iagatti; Lorenzo Cupellini; Giacomo Biagiotti; Stefano Caprasecca; Stefano Fedeli; Andrea Lapini; Eleonora Ussano; Stefano Cicchi; Paolo Foggi; Massimo Marcaccio; Benedetta Mennucci; Mariangela Di Donato
ChemPlusChem | 2015
Giulia Tuci; Lapo Luconi; Andrea Rossin; Francesco Baldini; Stefano Cicchi; Sara Tombelli; Cosimo Trono; Ambra Giannetti; Ilse Manet; Stefano Fedeli; Alberto Brandi; Giuliano Giambastiani
Journal of Drug Delivery Science and Technology | 2018
Giacomo Biagiotti; Maria Cristina Ligi; Stefano Fedeli; Erica Pranzini; Tania Gamberi; Stefano Cicchi; Paolo Paoli