Stephan Sehmisch

Standardized Bending and Breaking Test for the Normal and Osteoporotic Metaphyseal Tibias of the Rat: Effect of Estradiol, Testosterone, and Raloxifene

The fracture of bone plays a key role in osteoporosis. BMD measurement, however, is only an indirect parameter of this phenomenon. We therefore developed a highly sensitive three‐point bending test for the metaphyseal tibias in rats to evaluate stiffness and strength. This was validated in a right‐left comparison and a bioassay with soy‐free food, estradiol, raloxifene, and testosterone in orchidectomized rats.

Estrogen and raloxifene improve metaphyseal fracture healing in the early phase of osteoporosis. A new fracture-healing model at the tibia in rat

BackgroundFracture healing in osteoporosis is delayed. Quality and speed of fracture healing in osteoporotic fractures are crucial with regard to the outcome of patients. The question arises whether established antiosteoporotic drugs can further improve fracture healing.Materials and methodsOsteoporosis manifests predominantly in the metaphyseal bone. Nevertheless, an established metaphyseal fracture model is lacking. A standardized metaphyseal fracture-healing model with stable plate fixation was developed for rat tibiae. The healing process was analyzed by biomechanical, gene expression, and histomorphometric methods in ovariectomized (OVX) and sham-operated rats (SHAM), compared to standardized estrogen (E)- and raloxifene (R)-supplemented diets.ResultsEstrogen and raloxifene improved the biomechanical properties of bone healing compared to OVX (Yield load:

Whole body vibration during fracture healing intensifies the effects of estradiol and raloxifene in estrogen-deficient rats

{\text{SHAM}} = 63.1 \pm 20.8{\text{N}}

Evaluation of bone quality and quantity in osteoporotic mice--the effects of genistein and equol.

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Effects of black cohosh (Cimicifuga racemosa) and estrogen on metaphyseal fracture healing in the early stage of osteoporosis in ovariectomized rats.

\operatorname{E} = 60.8 \pm 17.9\operatorname{N}

Impact of 4-methylbenzylidene camphor, daidzein, and estrogen on intact and osteotomized bone in osteopenic rats

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