Stephan Wnendt
Astellas Pharma
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Publication
Featured researches published by Stephan Wnendt.
Journal of Cardiovascular Pharmacology | 2006
Christian M. Matter; Izabela Rozenberg; Andrea Jaschko; Helen Greutert; David J. Kurz; Stephan Wnendt; Bernd Kuttler; Hana Joch; Jürg Grünenfelder; Gregor Zünd; Felix C. Tanner; Thomas F. Lüscher
Abstract: Local strategies directed against vascular smooth muscle cell (VSMC) proliferation such as drug-eluting stents reduce the occurrence of restenosis. However, these approaches may also inhibit endothelial cell (EC) proliferation and, thus, impair reendothelialization. We compared the effects of tacrolimus on human VSMC and EC proliferation and migration to sirolimus, a compound with similar molecular structure. Thymidine incorporation was determined in growth factor-stimulated VSMC and EC. The drug concentration at which maximal VSMC proliferation was inhibited by 50% (IC50) was about 102-fold higher for tacrolimus (3.8 × 10−7 M) than for sirolimus (4.1 × 10−9 M; P = 0.055). It is interesting that the molar IC50 value in EC was around 104-fold higher for tacrolimus (2.3 × 10−6 M) than for sirolimus (7.1 × 10−10 M; P < 0.01). The profile of these drugs on VSMC and EC migration was similar to the one found in the proliferation assays. Inhibition of VSMC proliferation by both tacrolimus and sirolimus was associated with upregulation of the cell-cycle inhibitor p27. Thus, tacrolimus is less potent than sirolimus for inhibiting VSMC proliferation or migration. However, tacrolimus exerts markedly less antiproliferative effects on EC compared with sirolimus. In combination with its potent antiinflammatory effects, tacrolimus may represent a promising compound for the use in drug-eluting stents.
Archive | 2003
Stephan Wnendt; Randolf Von Oepen; Bernd Kuttler; Gerhard Lang; Guenter Lorenz; Axel Grandt
Archive | 2002
Stephan Wnendt; Oepen Randolf Von; Bernd Kuttler; Gerhard Lang
Archive | 2001
Stephan Wnendt; Randolf Von Oepen; Bernd Kuttler; Gerhard Lang
Archive | 2003
Stephan Wnendt; Randolf Von Oepen; Bernd Kuttler; Gerhard Lang; Guenter Lorenz; Axel Grandt
Archive | 2002
David Chaplin; Stephan Wnendt; Bernd Kuttler; Gunter Lorenz
Archive | 2009
Randolf Von Oepen; Bernd Kuttler; Gerhard Lang; Stephan Wnendt
Archive | 2002
Stephan Wnendt; Oepen Randolf Von; Bernd Kuttler; Gerhard Lang
Archive | 2002
Stephan Wnendt; Oepen Randolf Von; Bernd Kuttler; Gerhard Lang
Archive | 2002
Stephan Wnendt; Oepen Randolf Von; Bernd Kuttler; Gerhard Lang