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Dive into the research topics where Steven W. Dodd is active.

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Featured researches published by Steven W. Dodd.


Archive | 1988

Engineering a Stable Protease

Philip N. Bryan; Michele L. Rollence; Jay F. Wood; Steven Quill; Steven W. Dodd; Mark Whitlow; Karl D. Hardman; Michael W. Pantoliano

We have used several approaches to engineer large increases in the stability of the Bacillus serine protease, subtilisin. These include introducing disulfide cross-links, improving electrostatic interactions at calcium ion binding sites, and the use of in vitro random mutagenesis coupled with a phenotypic screen to identify stabilizing mutational events. More than twenty individual stabilizing mutations of subtilisin BPN’ have been identified. Thermodynamic analysis has shown that individually these modifications contribute between 0.3–1.5 Kcal/mol to the free energy of stabilization. We have further found that combining individual stabilizing mutations results in cumulative increases in stability. Calorimetric and crystallographic data demonstrate that increases in the free energy of stabilization are often independent and additive. We therefore have been able to create extremely stable versions of subtilisins in a step by step manner. Thermodynamic stability of subtilisin was also shown to be related to resistance to irreversible inactivation at high temperature and high pH. The most stable versions have half-lives at high pH or high temperature approaching 1000-times longer than the wild type subtilisin BPN’.


Journal of the National Cancer Institute | 1990

In Vivo Tumor Targeting of a Recombinant Single-Chain Antigen-Binding Protein

David Colcher; Robert E. Bird; Mario Roselli; Karl D. Hardman; Syd Johnson; Sharon Pope; Steven W. Dodd; Michael W. Pantoliano; Diane E. Milenic; Jeffrey Schlom


Biochemistry | 1991

Conformational stability, folding, and ligand-binding affinity of single-chain Fv immunoglobulin fragments expressed in Escherichia coli.

Michael W. Pantoliano; Robert E. Bird; Syd Johnson; Eric D. Asel; Steven W. Dodd; Jay F. Wood; Karl D. Hardman


Biochemistry | 1989

Large increases in general stability for subtilisin BPN' through incremental changes in the free energy of unfolding.

Michael W. Pantoliano; Marc Whitlow; Jay F. Wood; Steven W. Dodd; Karl D. Hardman; Michele L. Rollence; Philip N. Bryan


Proceedings of the National Academy of Sciences of the United States of America | 1991

CONSTRUCTION AND CHARACTERIZATION OF A SINGLE-CHAIN CATALYTIC ANTIBODY

Richard A. Gibbs; Bruce A. Posner; David Filpula; Steven W. Dodd; Malcolm A. J. Finkelman; Timothy K. Lee; Marie Wroble; Marc Whitlow; Stephen J. Benkovic


Archive | 2001

Compositions of peptide crystals

Steven W. Dodd; Rohn Lee Junior Millican; Joseph Vincent Junior Rinella


Biochemistry | 1996

Hierarchical modeling of phenolic ligand binding to 2Zn--insulin hexamers.

Duane T. Birnbaum; Steven W. Dodd; Bo E. Saxberg; Alexander D. Varshavsky; John Michael Beals


Archive | 1997

Obesity protein formulations

John Michael Beals; Steven W. Dodd; Allen H. Pekar


Archive | 2001

Compositions de cristaux de peptides

Steven W. Dodd; Rohn Lee Junior Millican; Joseph Vincent Junior Rinella


Archive | 1997

Préparation des protéines contre l'obésité

John Michael Beals; Steven W. Dodd; Rohn Lee Junior Millican; Allen H. Pekar

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Jay F. Wood

Walter Reed Army Institute of Research

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Marc Whitlow

Pennsylvania State University

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Philip N. Bryan

University of Maryland Biotechnology Institute

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