Sun Ok Jung
Korea Research Institute of Bioscience and Biotechnology
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Publication
Featured researches published by Sun Ok Jung.
Analytical Chemistry | 2007
Tai Hwan Ha; Sun Ok Jung; Jeong Min Lee; Kun Yeong Lee; Yan Lee; Jong Sang Park; Bong Hyun Chung
We have constructed a novel platform for the oriented buildup of immunoglobulins on a gold surface for a surface plasmon resonance imaging microarray. To this end, genetically engineered glutathione S-transferase proteins bearing one, two, and three Fc-specific B-domains in protein G from Streptococci (GST-GB1, -GB2, and -GB3, respectively) were produced. In order to tether these GST-GBx proteins specifically, a novel glutathione-derivatized ligand (LA-GSH) was also synthesized from a biaminated tri(ethylene glycol) backbone. Each end of the backbone was further functionalized with a maleimide group for a glutathione modification and a lipoic acid for surface immobilization. The glutathione ligand demonstrated a negligible nonspecific protein adsorption toward other spectator proteins while showing a strong specific association toward GST-GBx proteins. This Fc-specific surface exhibited at least a 2-fold enhancement in the immunoglobulin density (from human and mouse) with its antigen capture capability totally conserved compared to a covalently tethered GBx proteins. A single antibody tethered on the GST-GB3 is estimated to capture two antigens (enhanced green fluorescent protein), and this antigen capture ratio seems to be the most efficient value ever observed.
European Biophysics Journal | 2006
Moonil Kim; Soyoung Lee; Hyunju Choi; Yong-Beom Shin; Sun Ok Jung; Min-Gon Kim; Bong Hyun Chung
In a recent study, we reported the results of a rapid high-throughput expression analysis of the affinity-tagged proteins present in total cell lysates, using a surface plasmon resonance (SPR) imaging protein chip system. In this paper, we describe a novel method, which is able to sequentially carry out a recombinant Escherichia coli culture, as well as the detection and purification of the expressed proteins on a single microwell chip, fabricated on a two-dimensional thin gold film. Following the induction of the protein on the microwell chip, the E. coli cells were lysed on the chip via the addition of lysozymes, and the expressed glutathione S-transferase-fused green fluorescent protein (GST–GFP) was then purified on the chip via affinity interaction with the glutathionylated gold surface of the chip. Finally, the expressed protein was directly detected using the surface plasmon resonance (SPR) imaging system. This system saves a substantial amount of time, experimental resources, and labor, by allowing for the complicated and labor-intensive procedures inherent to the production of recombinant proteins to be conducted on a single microwell chip, simply and economically.
Applied and Environmental Microbiology | 2005
Hyeon-Su Ro; Sun Ok Jung; Byung Hoon Kho; Hyung Pyo Hong; Jae Sung Lee; Yong-Beom Shin; Min Gon Kim; Bong Hyun Chung
ABSTRACT A surface plasmon resonance imaging-based Ni2+-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins.
Analytical Chemistry | 2007
Jeong Min Lee; Hyun Park; Yongwon Jung; Jin Kyeong Kim; Sun Ok Jung; Bong Hyun Chung
Analytical Biochemistry | 2008
Yongwon Jung; Hyo Jin Kang; Jeong Min Lee; Sun Ok Jung; Wan Soo Yun; Sang J. Chung; Bong Hyun Chung
Proteomics | 2005
Sun Ok Jung; Hyeon-Su Ro; Byung Hoon Kho; Yong-Beom Shin; Min Gon Kim; Bong Hyun Chung
Proteomics | 2006
Hyeon-Su Ro; Byung Hoon Koh; Sun Ok Jung; Hyun Kyu Park; Yong-Beom Shin; Min-Gon Kim; Bong Hyun Chung
Biochemical and Biophysical Research Communications | 2005
Moonil Kim; Sun Ok Jung; Kyoungsook Park; Eun-Ju Jeong; Hyou-Arm Joung; Tae-Hyoung Kim; Dai-Wu Seol; Bong Hyun Chung
Archive | 2007
Bong Hyun Chung; Tai Hwan Ha; Sun Ok Jung; Sang Jeon Chung; Suh Hyun Lee; Jung-Won Kim
한국생물공학회 학술대회 | 2005
Soyoung Lee; Moonil Kim; Hyunju Choi; Yong-Beom Shin; Sun Ok Jung; Min-Gon Kim; Bong Hyun Chung
Collaboration
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Korea Research Institute of Bioscience and Biotechnology
View shared research outputsKorea Research Institute of Bioscience and Biotechnology
View shared research outputsKorea Research Institute of Bioscience and Biotechnology
View shared research outputsKorea Research Institute of Bioscience and Biotechnology
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