Susana P.F. Costa
University of Porto
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Featured researches published by Susana P.F. Costa.
Ecotoxicology and Environmental Safety | 2012
Paula C.A.G. Pinto; Susana P.F. Costa; José L. F. C. Lima; M. Lúcia M.F.S. Saraiva
An automated high-throughput Vibrio fischeri assay was developed and further applied to the evaluation of ionic liquids (ILs) (eco)toxicity. The assay was based on the reduction of bacterial bioluminescence in the presence of test compounds and the results were presented as EC(50). The assays were performed with eight commercially available ILs with distinct cationic head groups, alkyl side chains and anions. EC(50) values between 6.5 and 691.9 mmol L(-1) were obtained for the tested ILs, being hmim [Cl] the most toxic and bmim [Cl] the less toxic ones, confirming the influence of the different structural elements. Moreover, all the tested ILs exhibited a (eco)toxicity lower than Cu(II), used as a positive control during the optimization and analysis steps. The automated assay assured the precise control of the contact time between V. fischeri and test compound by means of a simple protocol that guaranteed adequate aspiration and handling of the solutions as well as the precise implementation of a computer controlled stop period. Furthermore, a significant reduction of the assay costs was achieved through automation mainly by a drastic reduction of the volume of bacterial suspension and test compound. The methodology was validated by comparison with a microplate assay; it was stated that the results, obtained after a 3min contact time, changed proportionally relatively to Cu(II) in both assays. This confirmed the applicability of the methodology as an (eco)toxicity screening assay, with reduction of time and increase of robustness and repeatability (n=10; rsd<1.1%). It is expected that due to its simplicity and reduced cost the developed assay can be integrated in the early stage of development of new compounds as a rapid screening test.
Journal of Hazardous Materials | 2015
Susana P.F. Costa; Paula C.A.G. Pinto; Rui A. S. Lapa; M. Lúcia M.F.S. Saraiva
A fully automated Vibrio fischeri methodology based on sequential injection analysis (SIA) has been developed. The methodology was based on the aspiration of 75 μL of bacteria and 50 μL of inhibitor followed by measurement of the luminescence of bacteria. The assays were conducted for contact times of 5, 15, and 30 min, by means of three mixing chambers that ensured adequate mixing conditions. The optimized methodology provided a precise control of the reaction conditions which is an asset for the analysis of a large number of samples. The developed methodology was applied to the evaluation of the impact of a set of ionic liquids (ILs) on V. fischeri and the results were compared with those provided by a conventional assay kit (Biotox(®)). The collected data evidenced the influence of different cation head groups and anion moieties on the toxicity of ILs. Generally, aromatic cations and fluorine-containing anions displayed higher impact on V. fischeri, evidenced by lower EC50. The proposed methodology was validated through statistical analysis which demonstrated a strong positive correlation (P>0.98) between assays. It is expected that the automated methodology can be tested for more classes of compounds and used as alternative to microplate based V. fischeri assay kits.
Chemsuschem | 2017
Susana P.F. Costa; Ana Azevedo; Paula C.A.G. Pinto; M. Lúcia M.F.S. Saraiva
This Review aims to integrate the most recent and pertinent data available on the (bio)degradability and toxicity of ionic liquids for global and critical analysis and on the conscious use of these compounds on a large scale thereafter. The integrated data will enable focus on the recognition of toxicophores and on the way the community has been dealing with them, with the aim to obtain greener and safer ionic liquids. Also, an update of the most recent biotic and abiotic methods developed to overcome some of these challenging issues will be presented. The review structure aims to present a potential sequence of events that can occur upon discharging ionic liquids into the environment and the potential long-term consequences.
Journal of Hazardous Materials | 2014
Susana P.F. Costa; Vanessa Dela Justina; Katharina Bica; Maria Vasiloiu; Paula C.A.G. Pinto; M. Lúcia M.F.S. Saraiva
The toxicity of 16 pharmaceutical active ionic liquids (IL-APIs) was evaluated by automated approaches based on sequential injection analysis (SIA). The implemented bioassays were centered on the inhibition of human carboxylesterase 2 and Vibrio fischeri, in the presence of the tested compounds. The inhibitory effects were quantified by calculating the inhibitor concentration required to cause 50% of inhibition (EC50). The EC50 values demonstrated that the cetylpyridinium group was one of the most toxic cations and that the imidazolium group was the less toxic. The obtained results provide important information about the safety of the studied IL-APIs and their possible use as pharmaceutical drugs. The developed automated SIA methodologies are robust screening bioassays, and can be used as a generic tools to identify the (eco)toxicity of the structural elements of ILs, contributing to a sustainable development of drugs.
Talanta | 2015
Carina F. Nascimento; Marcos A.S. Brasil; Susana P.F. Costa; Paula C.A.G. Pinto; Maria Lúcia M. F. S. Saraiva; Fábio R.P. Rocha
Formaldehyde is often added to foods as a preservative, but it is highly toxic to humans, having been identified as a carcinogenic substance. It has also been used for the adulteration of milk in order to diminish the bacteria count and increase the shelf life of the product. Herein, we present a green dispersive liquid-liquid microextraction procedure in a flow-batch system for the determination of formaldehyde in milk. Pulsed flows were exploited for the first time to improve the dispersion of the extractant in the aqueous phase. The Hantzsch reaction was used for the derivatization of formaldehyde and the product was extracted with the ionic liquid (IL) trihexyltetradecylphosphonium chloride with methanol as the disperser. The flow-batch chamber was made of stainless steel with the facility for resistive heating to speed up the derivatization reaction. Spectrophotometric measurements were directly carried out in the organic phase using an optical fiber spectrophotometer. The limit of detection and coefficient of variation were 100 μg L(-1) and 3.1% (n=10), respectively, with a linear response from 0.5 to 5.0 mg L(-1), described by the equation A=0.088+0.116CF (mg L(-1)) in which A is absorbance and CF is formaldehyde concentration in mg L(-1). The estimated recoveries of formaldehyde from spiked milk samples ranged from 91% to 106% and the slopes of the analytical curves obtained with reference solutions in water or milk were in agreement, thus indicating the absence of matrix effects. Accuracy was demonstrated by the agreement of the results with those achieved by the reference fluorimetric procedure at the 95% confidence level. The proposed procedure allows for 10 extractions per hour, with minimized reagent consumption (120 μL of IL and 3.5 μL acetylacetone) and generation of only 6.7 mL waste per determination, which contribute to the eco-friendliness of the procedure.
Journal of Hazardous Materials | 2016
Susana P.F. Costa; Bárbara S.F. Martins; Paula C.A.G. Pinto; M. Lúcia M.F.S. Saraiva
A fully automated cytochrome c oxidase assay resorting to sequential injection analysis (SIA) was developed for the first time and implemented to evaluate potential toxic compounds. The bioassay was validated by evaluation of 15 ionic liquids (ILs) with distinct cationic head groups, alkyl side chains and anions. The assay was based on cytochrome c oxidase activity reduction in presence of tested compounds and quantification of inhibitor concentration required to cause 50% of enzyme activity inhibition (EC50). The obtained results demonstrated that enzyme activity was considerably inhibited by BF4 anion and ILs incorporating non-aromatic pyrrolidinium and tetrabutylphosphonium cation cores. Emim [Ac] and chol [Ac], on contrary, presented the higher EC50 values among the ILs tested. The developed automated SIA methodology is a simple and robust high-throughput screening bioassay and exhibited good repeatability in all the tested conditions (rsd<3.7%, n=10). Therefore, it is expected that due to its simplicity and low cost, the developed approach can be used as alternative to traditional screening assays for evaluation of ILs toxicity and identification of possible toxicophore structures. Additionally, the results presented in this study provide further information about ILs toxicity.
Helicobacter | 2015
Ceu Figueiredo; Susana P.F. Costa; Andreas Karameris; José Carlos Machado
Gastric cancer (GC) is the fifth most common malignancy and the third leading cause of cancer‐related death worldwide. GC is a heterogeneous disease and the endpoint of a long multistep process largely influenced by Helicobacter pylori infection, genetic susceptibility, and environmental factors. In a subset of GC cases, infection with the Epstein–Barr virus (EBV) may also be involved. The development of GC is the consequence of the accumulation of multiple epi/genetic changes during the patients lifetime that will result in oncogenic activation and/or tumor suppressor pathways’ inactivation.
Talanta | 2012
Paula C.A.G. Pinto; Susana P.F. Costa; José L. F. C. Lima; M. Lúcia M.F.S. Saraiva
An automated methodology for the kinetic study of β-galactosidase activity in sodium dodecylsulfate (SDS)/ionic liquid (IL) mixed micelles was developed. The main objective of the work was the evaluation of mixed micelles as reaction media for the industrial synthesis of glyco-oligossacharides. Enzyme activity was evaluated by means of a model reaction with the fluorescent substrate 4-methylumbelliferyl-α-d-galactopyranoside (MUG). The assay was implemented in a sequential injection analysis (SIA) system and enzyme activity was studied in SDS/bmim [BF(4)] and SDS/hmim [Cl] mixed micelles with variable concentrations of both components. In order to perform a critical evaluation of the obtained results, CMC and average micellar size of SDS/hmim [Cl] mixed micelles were evaluated by fluorescence and dynamic light scattering, respectively. In the micelle characterization assays it was observed that the CMC of the mixed micelles increased with hmim [Cl] concentration up to 1molL(-1). In the presence of higher IL concentrations there were no evidences of micelle formation. Regarding micellar size, it was maximum for an IL concentration of 0.09molL(-1). The kinetic assays evidenced that SDS/bmim [BF(4)] and SDS/hmim [Cl] mixed micellar systems can led to an increase of enzyme activity. This increase is dependent on the variation of the average micellar size that occurs with the increase of IL concentration up to 0.09molL(-1). It was also noticed that the most promising systems are those incorporating SDS and IL in concentrations under 50mmolL(-1) and 0.5molL(-1), respectively(.) These results evidenced that the studied ILs can modify the physico-chemical properties of the surfactant solution in a favourable way regarding β-galactosidase activity being an important achievement for the future implementation of industrial processes catalyzed by this enzyme, mainly the synthesis of glyco-oligossacharides. Indeed, surfactant/IL mixed micelles proved to be an interesting alternative to conventional organic solvents in this field enabling the implementation of the processes in a relatively hydrophobic media with enhanced enzyme activity.
ChemPhysChem | 2015
S. Sofia M. Rodrigues; David S.M. Ribeiro; Christian Frigerio; Susana P.F. Costa; João A.V. Prior; Paula C.A.G. Pinto; João L.M. Santos; M. Lúcia M.F.S. Saraiva; Marieta L.C. Passos
Immobilization of quantum dots (QDs) onto solid supports could improve their applicability in the development of sensing platforms and solid-phase reactors by allowing the implementation of reusable surfaces and the execution of repetitive procedures. As the reactivity of QDs relies mostly on their surface chemistry, immobilization could also limit the disruption of solution stability that could prevent stable measurements. Herein, distinct strategies to immobilize QDs onto porous aminated supports, such as physical adsorption and the establishment of chemical linking, were evaluated. This work explores the influence of QD capping and size, concentration, pH, and contact time between the support and the QDs. Maximum QD retention was obtained for physical adsorption assays. Freundlich and Langmuir isotherms were used to analyze the equilibrium data. Gibbs free energy, enthalpy, and entropy were calculated and the stability of immobilized QDs was confirmed.
Ecotoxicology and Environmental Safety | 2018
Sarah A. P. Pereira; Susana P.F. Costa; Edite Cunha; Marieta L.C. Passos; André R.S.T. Araújo; M. Lúcia M.F.S. Saraiva
Antipsychotic (AP) drugs are becoming accumulated in terrestrial and aqueous resources due to their actual consumption. Thus, the search of methods for assessing the contamination load of these drugs is mandatory. The COD is a key parameter used for monitoring water quality upon the assessment of the effect of polluting agents on the oxygen level. Thus, the present work aims to assess the chemical oxygen demand (COD) levels of several typical and atypical antipsychotic drugs in order to obtain structure-activity relationships. It was implemented the titrimetric method with potassium dichromate as oxidant and a digestion step of 2h, followed by the measurement of remained unreduced dichromate by titration. After that, an automated sequential injection analysis (SIA) method was, also, used aiming to overcome some drawbacks of the titrimetric method. The results obtained showed a relationship between the chemical structures of antipsychotic drugs and their COD values, where the presence of aromatic rings and oxidable groups give higher COD values. It was obtained a good compliance between the results of the reference batch procedure and the SIA system, and the APs were clustered in two groups, with the values ratio between the methodologies, of 2 or 4, in the case of lower or higher COD values, respectively. The SIA methodology is capable of operating as a screening method, in any stage of a synthetic process, being also more environmentally friendly, and cost-effective. Besides, the studies presented open promising perspectives for the improvement of the effectiveness of pharmaceutical removal from the waste effluents, by assessing COD values.