Syarifuddin Wahid

Association between asthma control and Interleukin-17F expression levels in adult patients with atopic asthma

Objectives: To investigate the correlation between Interleukin 17 (IL-17F) and the level of asthma control. Methods: This is a cross-sectional study of 40 subjects who were diagnosed with atopic asthma. All participants were recruited from the Allergy and Immunology Clinic, Prof. R.D. Kandou General Hospital, Manado, Indonesia, between April 2015 and April 2016. Total serum IL-17F measured by using Enzyme-Linked Immunosorbent Assay methods; and mRNA IL-17F was obtained by using real-time reverse transcriptase polymerase chain reaction. Level of asthma control was quantified by using asthma control test (ACT) scoring system. The correlation between IL-17F, mRNA, and level of asthma control was analyzed by using Pearson’s correlation coefficient (r). Results: There is a strong positive correlation between IL-17F serum level and Nathan’s ACT-score (r=0.969) which is statistically significant (p<0.001). Analysis of the correlation between mRNA IL-17F serum level and Nathan’s ACT-score revealed a strong positive correlation (r=0.963), which is statistically significant (p<0.001). Conclusion: These findings suggest that IL-17F plays an important role in asthma control. However, the role played by IL-17F in asthma pathogenesis are still questions to be answered.

The Relationship between Total Ige and Neutrophils Count with Degrees of Asthma Control in Adult Atopic Patients

Introduction: Asthma is a chronic reversible inflammation which is caused by bronchial hypereactivity due to exposure to allergen and hyperproduction of certain specific IgE. Neutrophil in respiratory tract and high level of IgE in the blood serum is known to play in complex mechanism in asthma development. The role of total IgE serum and neutrophil serum in asthma development is still not well known. Aim: This study aim is to assess the correlation between level of total IgE and neutrophil serum with degree of asthma control. Methods: This is a cross sectional study with 35 adult subjects with atophy asthma (age 22-61), all of participant reside in Manado and its surrounding area. Degree of asthma control is categorized as intermittent, mild persistent, moderate persistent and severe persistent based on criteria by GINA. Patient with malignancy, sepsis, lung tuberculosis, diabetes mellitus, pregnant, and late stage chronic kidney disease are excluded from this study. Result: In this study there is no significant correlation between total IgE serum level and degree of asthma control (p>0.05.) also there is no significant correlation between each total IgE level and each degree of asthma control. There is significant positive correlation between neutrophil count and degree of asthma control (r = 0.309; pConclusion: The neutrophil count in serum is related to degree of asthma control. Especially neutrophil count in mild persistent is higher than intermittent asthma. There is no correlation between total IgE level with degree of asthma control in adult subject with atopic asthma.

Correlation between Levels of Transforming Growth Factor Beta 1 (TGF-β1) serum with Clinical Outcome on Acute Anterior Circulation Ischemic Strokes

Transforming Growth Factor Beta (TGFβ) was a major regulatory molecule to suppress the immune response in the inflammatory process. TGFβ was also a growth factor that affects growth, homeostasis, angiogenesis and tissue repair. In the acute phase of stroke, astrocytes were activated and the cells were able to produce anti-inflammatory cytokines such as TGFβ. The purpose of this study was to determine whether there is a correlation between serum levels of TGFβ at acute phase of ischemic stroke and patients’ clinical outcomes. The study was conducted in patients with acute anterior system ischemic stroke who came to Siloam Hospital in Tangerang, Indonesia. Blood samples were taken to measure the levels of TGFβ-1serum at ≤ 72 hours and the 3rd day of onset. Clinical severity of stroke assessed using the National Institute of Health (NIH) Stroke Scale at 72 hours, 7th days and 30th days after stroke. The mean serum levels of TGFβ-1 at ≤ 72 hours in the group of subjects with mild NIH Stroke Scale degree was higher than in the group of subjects with moderate/severe NIH Stroke Scale degree (p = 0.046). The subjects with elevated levels of TGF-β1 in the acute phase of stroke had better clinical degrees at the 30th day after the stroke, although statistically was not significant (p = 0.241). Result of this study showed that TGFβ-1 may act as a neuroprotector against brain tissue damage after ischemic stroke.

Expression of protein regenerating liver-3 (PRL-3) and VEGF in ovarian epithelial cancer and metastatic site

Background: PRL-3 (phosphatase of regenerating liver-3) and VEGF have been reported to play a role in the growth and metastasize of some cancers, including ovarian cancer. Aims: To investigate the expression of PRL-3 and VEGF in ovarian epithelial cancer, metastatic tumor and correlate with the clinicopathologic parameters. Methods: Expressions of PRL-3 and VEGF in 40 ovarian epithelial cancer specimens and 25 omentum metastasis site were detected by immunohistochemistry. RT-PCR was also performed to detect PRL-3 mRNA from ascites. Results: PRL-3 mRNA expression was clearly detected in the ascites of ovarian cancer patient positive metastasis compare with ovarian cancer without metastasis. Among ovarian epithelial cancer, there were 7 (17.5%) endometrioid, 11 (27.5%) mucinous and 22 (55%) samples of serous tumor. The expression of PRL-3 and VEGF was significantly higher in the primary ovarian epithelial cancer with omentum metastasis than primary tumor without metastasis (p = 0.031, p = 0.036). There was no significant correlation between PRL-3, VEGF expression and histological type, histological grade, and stadium. Conclusions: These results strongly suggest that PRL-3 and VEGF may play a significant role in invasion and metastasis of ovarian epithelial cancer. PRL-3 might be a novel molecular marker for aggressive ovarian cancer and act as a poor prognostic factor.

Expression of protein regenerating liver-3 (PRL-3) and E-Cadherin in metastatic breast cancer

Background: Breast cancer is the most common malignant neoplasm worldwide. Recently, PRL-3 (phosphatase of regenerating liver-3/PTP4A3) was reported to participate in the progression of breast cancer, but the mechanism still needs clarification. Aims: To investigate the expression of PRL-3 and E-Cadherin in breast cancer and metastatic tumor and correlate with the clinicopathologic parameters. Methods: Expressions of PRL-3 and E-Cadherin in 114 primary breast cancer specimens and 73 lymph node metastasis were detected by immunohistochemistry. Results: Among primary breast cancers examined, there were 11 (9.6%) low grade, 70 (61.4%) moderate grade and 33 (28.9%) high grade malignancy. There was significant correlation between PRL-3 expression and histological grading of breast cancer (p = 0.044) and the presence of LN metastasis (p < 0.001). The significant correlation was also found between expression of E-cadherin and the presence of LN metastasis (p = 0.001). The expression of PRL-3 in primary site was significantly correlated with metastatic site (p < 0.001). Then, the result showed that the higher the PRL-3 expression the lower the expression of E-cadherin in breast cancer. Conclusions: These results strongly suggest that PRL-3 plays a role in the progression and metastasis of breast cancer. PRL-3 may down-regulate the expression of E-cadherin and induce metastatic ability in breast cancer.