Sylvie Sauvaigo
Joseph Fourier University
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Sylvie Sauvaigo.
Archives of Biochemistry and Biophysics | 2011
Christophe Bounaix Morand du Puch; Ewa Barbier; Alexandra Kraut; Yohann Couté; Julia Fuchs; Arnaud Buhot; Thierry Livache; Michel Seve; Alain Favier; Thierry Douki; Didier Gasparutto; Sylvie Sauvaigo; Jean Breton
Platinating agents are commonly prescribed anticancer drugs damaging DNA. Induced lesions are recognized by a wide range of proteins. These are involved in cellular mechanisms such as DNA repair, mediation of cytotoxicity or chromatin remodeling. They therefore constitute crucial actors to understand pharmacology of these drugs. To expand our knowledge about this subproteome, we developed a ligand fishing trap coupled to high throughput proteomic tools. This trap is made of damaged plasmids attached to magnetic beads, and was exposed to cell nuclear extracts. Retained proteins were identified by nanoHPLC coupled to tandem mass spectrometry. This approach allowed us to establish a list of 38 proteins interacting with DNA adducts generated by cisplatin, oxaliplatin and satraplatin. Some of them were already known interactome members like high mobility group protein 1 (HMGB1) or the human upstream binding factor (hUBF), but we also succeeded in identifying unexpected proteins such as TOX HMG box family member 4 (TOX4), phosphatase 1 nuclear targeting subunit (PNUTS), and WD repeat-containing protein 82 (WDR82), members of a recently discovered complex. Interaction between TOX4 and platinated DNA was subsequently validated by surface plasmon resonance imaging (SPRi). These interactions highlight new cellular responses to DNA damage induced by chemotherapeutic agents.
Archive | 1990
Robert Teoule; Thierry Livache; Brigitte Fouque; Sylvie Sauvaigo
Archive | 1993
Robert Teoule; Thierry Livache; Brigitte Fouque; Sylvie Sauvaigo
Archive | 1990
Robert Teoule; André Roget; Hervé Bazin; Sylvie Sauvaigo
Archive | 1992
Sylvie Sauvaigo; Brigitte Fouque
Archive | 1995
Sylvie Sauvaigo; Hervé Bazin; Thierry Livache
Archive | 1998
Robert Teoule; Sylvie Sauvaigo; Hervé Bazin
Archive | 1990
Robert Teoule; André Roget; Hervé Bazin; Sylvie Sauvaigo
Archive | 1995
Hervé Bazin; Thierry Livache; Sylvie Sauvaigo
Archive | 1989
Robert Teoule; Thierry Livache; Brigitte Fouque; Sylvie Sauvaigo