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Dive into the research topics where Sylvie Sauvaigo is active.

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Featured researches published by Sylvie Sauvaigo.


Archives of Biochemistry and Biophysics | 2011

TOX4 and its binding partners recognize DNA adducts generated by platinum anticancer drugs.

Christophe Bounaix Morand du Puch; Ewa Barbier; Alexandra Kraut; Yohann Couté; Julia Fuchs; Arnaud Buhot; Thierry Livache; Michel Seve; Alain Favier; Thierry Douki; Didier Gasparutto; Sylvie Sauvaigo; Jean Breton

Platinating agents are commonly prescribed anticancer drugs damaging DNA. Induced lesions are recognized by a wide range of proteins. These are involved in cellular mechanisms such as DNA repair, mediation of cytotoxicity or chromatin remodeling. They therefore constitute crucial actors to understand pharmacology of these drugs. To expand our knowledge about this subproteome, we developed a ligand fishing trap coupled to high throughput proteomic tools. This trap is made of damaged plasmids attached to magnetic beads, and was exposed to cell nuclear extracts. Retained proteins were identified by nanoHPLC coupled to tandem mass spectrometry. This approach allowed us to establish a list of 38 proteins interacting with DNA adducts generated by cisplatin, oxaliplatin and satraplatin. Some of them were already known interactome members like high mobility group protein 1 (HMGB1) or the human upstream binding factor (hUBF), but we also succeeded in identifying unexpected proteins such as TOX HMG box family member 4 (TOX4), phosphatase 1 nuclear targeting subunit (PNUTS), and WD repeat-containing protein 82 (WDR82), members of a recently discovered complex. Interaction between TOX4 and platinated DNA was subsequently validated by surface plasmon resonance imaging (SPRi). These interactions highlight new cellular responses to DNA damage induced by chemotherapeutic agents.


Archive | 1990

Method for detecting specific nucleic acid sequences and applications of same

Robert Teoule; Thierry Livache; Brigitte Fouque; Sylvie Sauvaigo


Archive | 1993

Method for detecting specific nucleic acid sequences by amplification in highly dilute solution

Robert Teoule; Thierry Livache; Brigitte Fouque; Sylvie Sauvaigo


Archive | 1990

Derivatives of polyhydroxylated molecules for introducing at least one ramification in an oligonucleotide

Robert Teoule; André Roget; Hervé Bazin; Sylvie Sauvaigo


Archive | 1992

Oligonucleotides useful as reactants for the in vitro detection of HIV retrovirus infections and HIV retrovirus detection method

Sylvie Sauvaigo; Brigitte Fouque


Archive | 1995

Method for the amplification and/or detection of a nucleic acid sequence, detection reagent and uses thereof

Sylvie Sauvaigo; Hervé Bazin; Thierry Livache


Archive | 1998

System for detecting nucleic acid hybridization, preparation method and application thereof

Robert Teoule; Sylvie Sauvaigo; Hervé Bazin


Archive | 1990

Derives de molecules polyhydroxylees permettant l'introduction d'au moins une ramification dans un oligonucleotide

Robert Teoule; André Roget; Hervé Bazin; Sylvie Sauvaigo


Archive | 1995

Méthode d'amplification et/ou de détection d'une séquence d'acide nucléique, réactif de détection et leurs applications

Hervé Bazin; Thierry Livache; Sylvie Sauvaigo


Archive | 1989

Process for the detection and/or identification of nucleic acids by amplification and hybridisation in solution, and its applications

Robert Teoule; Thierry Livache; Brigitte Fouque; Sylvie Sauvaigo

Collaboration


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Thierry Livache

Centre national de la recherche scientifique

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Hervé Bazin

Institut Gustave Roussy

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André Roget

Centre national de la recherche scientifique

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Thierry Douki

Centre national de la recherche scientifique

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Alain Favier

Joseph Fourier University

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Alexandra Kraut

Joseph Fourier University

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Ewa Barbier

Joseph Fourier University

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Jean Breton

Joseph Fourier University

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Angélique Simon-Deckers

Centre national de la recherche scientifique

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