T.A. Wickersham

Effect of rumen-degradable intake protein supplementation on urea kinetics and microbial use of recycled urea in steers consuming low-quality forage

We evaluated the effect of increasing amounts of rumen-degradable intake protein (DIP) on urea kinetics in steers consuming prairie hay. Ruminally and duodenally fistulated steers (278 kg of BW) were used in a 4 x 4 Latin square and provided ad libitum access to low-quality prairie hay (4.9% CP). The DIP was provided as casein dosed ruminally once daily in amounts of 0, 59, 118, and 177 mg of N/kg of BW daily. Periods were 13 d long, with 7 d for adaptation and 6 d for collection. Steers were in metabolism crates for total collection of urine and feces. Jugular infusion of (15)N(15)N-urea, followed by determination of urinary enrichment of (15)N(15)N-urea and (14)N(15)N-urea was used to determine urea kinetics. Forage and N intake increased (linear, P < 0.001) with increasing DIP. Retention of N was negative (-2.7 g/d) for steers receiving no DIP and increased linearly (P < 0.001; 11.7, 23.0, and 35.2 g/d for 59, 118, and 177 mg of N/kg of BW daily) with DIP. Urea synthesis was 19.9, 24.8, 42.9, and 50.9 g of urea-N/d for 0, 59, 118, and 177 mg of N/kg of BW daily (linear, P = 0.004). Entry of urea into the gut was 98.9, 98.8, 98.6, and 95.9% of production for 0, 59, 118, and 177 mg of N/kg of BW daily, respectively (quadratic, P = 0.003). The amount of urea-N entering the gastrointestinal tract was greatest for 177 mg of N/kg of BW daily (48.6 g of urea-N/d) and decreased (linear, P = 0.005) to 42.4, 24.5, and 19.8 g of urea-N/d for 118, 59, and 0 mg of N/kg of BW daily. Microbial incorporation of recycled urea-N increased linearly (P = 0.02) from 12.3 g of N/d for 0 mg of N/kg of BW daily to 28.9 g of N/d for 177 mg of N/kg of BW daily. Provision of DIP produced the desired and previously observed increase in forage intake while also increasing N retention. The large percentage of urea synthesis that was recycled to the gut (95.9% even when steers received the greatest amount of DIP) points to the remarkable ability of cattle to conserve N when fed a low-protein diet.

Effect of a wide range in the ratio of supplemental rumen degradable protein to starch on utilization of low-quality, grass hay by beef steers

Hereford×Angus steers were used in a 14-treatment, 2-period, crossover design experiment to examine effects of a wide range in the ratio of supplemental starch to rumen degradable protein (RDP) on low-quality forage utilization and ruminal characteristics. Steers were given ad libitum access to grass hay (4.9% CP, 42.4% ruminally degradable) and supplemented in a 2×7 factorial arrangement of treatments. All supplements were administered directly into the rumen and delivered one of two levels of ruminally degradable starch (cornstarch grits; 0 and 0.3% of initial BW) and one of seven levels of RDP (sodium caseinate; 0, 0.015, 0.051, 0.087, 0.123, 0.159, and 0.195% of initial BW). Supplementation with RDP increased consumption of forage OM, total OM, NDF, and digestible OM in a quadratic (P<0.01) fashion (intake increased and then declined). Starch supplementation depressed (P<0.01) forage OM and NDF intakes. In general, RDP supplementation elicited a positive quadratic response on NDF digestion (P=0.02). However, an interaction between supplemental starch and RDP level was observed (P<0.01) for NDF digestion. At the four lowest levels of supplemental RDP, starch supplementation substantially reduced NDF digestion, although for steers receiving the three highest levels of supplemental RDP, starch supplementation had little effect on NDF digestion. In contrast, neither the starch×RDP interaction nor the starch main effect was significant for OM digestion, which increased linearly (P<0.01) with supplemental RDP. Supplementation with RDP altered passage rate of acid detergent insoluble ash in a quadratic (P=0.05) manner that paralleled the intake response. However, liquid passage rate was not affected significantly. A decline in ruminal pH was associated (P=0.02) with increasing supplemental RDP and tended (P=0.07) to be associated with increasing starch, but it was not requisite for starch-induced depressions in NDF digestion. Ruminal NH3 concentration increased in response to increasing RDP, although the increase when starch was supplemented was less than that observed without starch (P=0.03). Supplemental starch generally elicited negative effects on low-quality forage intake and fiber digestion, but the effects on fiber digestion were overridden by adequate supplemental RDP. Supplemental RDP exerted a highly positive effect on consumption and digestion of this low-quality forage.

Effect of frequency and amount of rumen-degradable intake protein supplementation on urea kinetics and microbial use of recycled urea in steers consuming low-quality forage.

We evaluated the effect of frequency and amount of rumen-degradable intake protein (DIP) on urea kinetics in steers consuming prairie hay. Five ruminally and duodenally fistulated steers (366 kg of BW) were used in a 5 x 5 Latin square and provided ad libitum access to low-quality prairie hay (4.7% CP). Casein was provided daily in amounts of 61 and 183 mg of N/kg of BW (61/d and 183/d) and every third day in amounts of 61, 183, and 549 mg of N/kg of BW per supplementation event (61/3d, 183/3d, and 549/3d). Periods were 18-d long with 9 d for adaptation and 9 d for collection. Steers were in metabolism crates for total collection of urine and feces. Jugular infusion of (15)N(15)N-urea followed by determination of urinary enrichment of (15)N(15)N-urea and (14)N(15)N-urea was used to determine urea kinetics. Treatment means were separated to evaluate the effects of increasing DIP supplementation and the effects of frequency at the low (61/d vs. 183/3d) and at the high (183/d vs. 549/3d) amounts of DIP provision. Forage OM and total digestible OM intakes were linearly (P < or = 0.05) increased by increasing DIP provision but were not affected by frequency of supplementation at either the low or high amounts. Production and gut entry of urea linearly (P < or = 0.006) increased with DIP provision and tended to be greater (P < or = 0.07) for 549/3d than 183/d but were not different between 61/d and 183/3d. Microbial N flow to the duodenum was linearly (P < 0.001) increased by increasing DIP provision. Additionally, 183/d resulted in greater (P = 0.05) microbial N flow than 549/3d. Incorporation of recycled urea-N into microbial N linearly (P = 0.04) increased with increasing DIP. Microbial incorporation of recycled urea-N was greater for 549/3d than 183/d, with 42 and 23% of microbial N coming from recycled urea-N, respectively. In contrast, there was no difference due to frequency in the incorporation of recycled urea-N by ruminal microbes at the low level of supplementation (i.e., 61/d vs. 183/3d). This study demonstrates that urea recycling plays a substantial role in the N supply to the rumen and to the animal, particularly in steers supplemented infrequently with high levels of protein.

Methodology for concurrent determination of urea kinetics and the capture of recycled urea nitrogen by ruminal microbes in cattle.

We measured the incorporation of recycled urea-nitrogen (N) by ruminal microbes, using five ruminally and duodenally fistulated steers (237 kg) fed low-quality grass hay (47 g crude protein/kg dry matter (DM)). Three received 1 kg/day of soybean meal (SBM) and two received no supplemental protein (control). The experiment was 15 days long. Background enrichments of 15N were measured on day 9 and continuous jugular infusion of 0.12 g/day [15N15N]urea began on day 10. Daily samples of urine, feces, ruminal bacteria and duodenal digesta from days 10 through 14 were used to determine plateaus in 15N enrichment. Duodenal and bacterial samples collected on day 15 were used to measure duodenal N flows. Bacterial N flow was calculated as duodenal N flow multiplied by duodenal 15N enrichment divided by bacterial 15N enrichment. Bacterial N from recycled urea-N was calculated as bacterial N flow multiplied by bacterial 15N enrichment divided by urinary urea 15N enrichment. Urinary enrichment of [15N15N]urea plateaued within 24 h, whereas 14N15N urea plateaued within 48 h of [15N15N]urea infusion. Bacteria reached a plateau in 15N enrichment within 24 h and duodenal samples within 48 h. Urea production was 17.6 g of urea-N/day for control and 78.0 g/day for SBM. Gut entry was 0.99 g of urea-N/g of urea-N produced for control and 0.87 g/g for SBM. Incorporation of recycled N into microbial N was 9.0 g of N/day for control and 23.0 g/day for SBM. Recycled urea-N accounted for 0.33 g of N/g of microbial N at the duodenum for control and 0.27 g/g for SBM. Our methods allowed measurement of incorporation of recycled urea-N into ruminal microbial N.

Determining the influence of different levelsof urea supplementation when beef cows grazing winter pasture are supplemented at different frequencies during the prepartum period

One hundred sixty spring-calving Hereford × Angus cows grazing lowquality, tallgrass-prairie range during the winter of 2000-2001 were supplemented before calving either daily or three times weekly. The supplement contained 40% CP with 0, 15, 30, or 45% of the supplemental degradable intake protein from urea. Supplement was fed at 4 lbs/head daily to cows receiving supplement daily. Cows receiving supplement three times weekly were fed the same amount of weekly supplement, but split equally among their supplementation events. After calving, all cows received a supplement without urea on a daily basis. In general, prepartum supplements that contained more urea prompted greater body weight loss; however, the effect of increasing urea was most noticeable when supplements were fed only three times weekly. When averaged across supplementation frequencies, increasing the level of supplemental urea tended (P=0.15) to decrease pregnancy rate in beef cows that had received urea supplementation before calving.

Microbial use of recycled urea is dependent on the level and frequency of degradable intake protein supplementation

Protein supplementation increases utilization (intake and digestion) of low-quality forage and ultimately animal performance. Despite its effectiveness, protein supplementation is often expensive. One strategy to reduce the cost of supplementation is to supplement less frequently than daily, generally every other day or every third day. By reducing the frequency of supplementation, the cost of delivering the supplement is reduced. Reducing the frequency of supplementation is an effective strategy for reducing cost, and it only minimally impacts animal performance, with less frequent supplementation resulting in slightly greater losses of body condition score and body weight during the winter supplementation period.

Supplementation with undegradable intake protein increases utilization of low-quality forage and microbial use of recycled urea

Low-quality forage utilization (intake and digestion) is improved by protein supplementation. Typically, the recommendation is to select supplements that are high in degradable intake protein because this fraction of the protein directly addresses the ruminal nitrogen deficiency that exists when low-quality forages are fed. However, the low cost of byproducts (e.g., distiller’s grains) that are high in undegradable intake protein makes them an attractive source of supplemental protein even though the response per unit of supplemental protein is less for undegradable protein than for degradable protein. One of the primary barriers to utilizing highly undegradable protein sources as supplements is the lack of information regarding their ability to provide nitrogen to ruminal microbes and, ultimately, their effectiveness as protein supplements to cattle fed low-quality forage. Because the protein is not ruminally degraded, the use of undegradable protein supplements to meet ruminal nitrogen requirements depends on the ability to recycle nitrogen to the rumen in the form of urea. Subsequently, the urea is utilized as a nitrogen source by ruminal microbes.

Supplementation with degradable intake protein increases low-quality forage utilization and microbial use of recycled urea

A common production practice throughout the United States is to supplement protein to cattle consuming low-quality forage (forage with a crude protein content of less than 7%) in order to improve animal performance (i.e., maintain body condition score and body weight) during the winter. Protein supplementation increases forage utilization (intake and digestion) and cow performance by supplying ruminal microbes with protein that is essential for microbial growth. Increased microbial activity in turn provides sources of both protein and energy to the cow. In addition to the protein that is fed and degraded in the rumen, ruminants have the ability to recycle urea—the same compound found in fertilizer and cattle feed—to the rumen, where microbes can use the urea to fulfill a portion of their nitrogen requirement. Although nutritionists know that recycling occurs, we have inadequate data to describe this process and, subsequently, the contribution from recycled urea is not adequately included in our present cattle feeding systems. Previous research at Kansas State University has clearly demonstrated that the greatest response to supplemental protein occurs when the supplemental protein is highly degraded within the rumen, as the degradable fraction of protein is directly available to ruminal microbes. The current project’s objective was to measure how much recycled urea is used to meet the microbial nitrogen requirement when increasing amounts of degradable intake protein were provided to steers consuming low-quality forage. Researchers hoped to generate data useful in refining supplementation recommendations for cattle consuming low-quality forage.

Effect of supplemental carbohydrate type and amount of ruminally degradable protein on utilization of tallgrass-prairie hay by beef steers

This experiment determined the impact of type of supplemental carbohydrate and amount of supplemental ruminally degradable protein (RDP) on intake and digestion of prairie hay. Fourteen ruminally fistulated beef steers were supplemented with one of two carbohydrates (corn starch or the simple sugar glucose) at 0.30% of body weight and one of seven levels of ruminally degradable protein (RDP; 0, 0.015, 0.051, 0.087, 0.123, 0.159, or 0.195% of body weight). Two additional steers served as controls (non-supplemented steers, i.e., no carbohydrate or RDP supplementation). Forage intake and digestion were substantially improved by increasing amounts of supplemental RDP. Supplemental carbohydrate with insufficient supplemental RDP depressed fiber digestion although carbohydrate type did not alter the digestion response or forage intake. In conclusion, when supplementing cattle eating low-quality forage, it is important to ensure that the supplement contains adequate RDP. The impact of the supplement on forage use should not differ greatly between starch (e.g., cereal grains) and sugar (e.g., molasses) as the main carbohydrate source.

Relative value of ruminally degradable and undegradable protein on the utilization of low-quality prairie hay by steers

An experiment was performed to investigate the impact of providing six levels of ruminally degradable protein (RDP; protein that is available to ruminal microbes) in combination with two levels of ruminally undegradable protein (RUP; protein that is not available to the ruminal microbes, but can be digested directly by cattle) on the intake and digestion of low-quality prairie hay. Twelve steers were provided unlimited access to low-quality prairie hay (5.3% crude protein and 71.7% neutral detergent fiber) throughout the trial. To simulate dietary RUP, casein was infused abomasally once daily at either 0 or 0.087% of body weight. To simulate dietary RDP, casein was infused ruminally once daily at 0, 0.029, 0.058, 0.087, 0.116, or 0.145% of body weight. As provision of RDP increased, forage intake and fiber digestion increased. Supplementing with RUP alone increased forage intake but not fiber digestion, although the intake response was not as large as providing the same amount of RDP. In conclusion, RUP is less efficient than RDP in stimulating forage intake and digestion.