T. Scott Isbell
University of Alabama at Birmingham
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Featured researches published by T. Scott Isbell.
Proceedings of the National Academy of Sciences of the United States of America | 2007
Gloria A. Benavides; Giuseppe L. Squadrito; Robert W. Mills; Hetal D. Patel; T. Scott Isbell; Rakesh P. Patel; Victor M. Darley-Usmar; Jeannette E. Doeller; David W. Kraus
The consumption of garlic is inversely correlated with the progression of cardiovascular disease, although the responsible mechanisms remain unclear. Here we show that human RBCs convert garlic-derived organic polysulfides into hydrogen sulfide (H2S), an endogenous cardioprotective vascular cell signaling molecule. This H2S production, measured in real time by a novel polarographic H2S sensor, is supported by glucose-maintained cytosolic glutathione levels and is to a large extent reliant on reduced thiols in or on the RBC membrane. H2S production from organic polysulfides is facilitated by allyl substituents and by increasing numbers of tethering sulfur atoms. Allyl-substituted polysulfides undergo nucleophilic substitution at the α carbon of the allyl substituent, thereby forming a hydropolysulfide (RSnH), a key intermediate during the formation of H2S. Organic polysulfides (R-Sn-R′; n > 2) also undergo nucleophilic substitution at a sulfur atom, yielding RSnH and H2S. Intact aorta rings, under physiologically relevant oxygen levels, also metabolize garlic-derived organic polysulfides to liberate H2S. The vasoactivity of garlic compounds is synchronous with H2S production, and their potency to mediate relaxation increases with H2S yield, strongly supporting our hypothesis that H2S mediates the vasoactivity of garlic. Our results also suggest that the capacity to produce H2S can be used to standardize garlic dietary supplements.
Journal of Clinical Investigation | 2007
John D. Lang; Xinjun Teng; Phillip Chumley; Jack H. Crawford; T. Scott Isbell; Balu K. Chacko; Yuliang Liu; Nirag Jhala; D. Ralph Crowe; Alvin B. Smith; Richard C. Cross; Luc Frenette; Eric E. Kelley; Diana W. Wilhite; Cheryl R. Hall; Grier P. Page; Michael B. Fallon; J. Steven Bynon; Devin E. Eckhoff; Rakesh P. Patel
Ischemia/reperfusion (IR) injury in transplanted livers contributes to organ dysfunction and failure and is characterized in part by loss of NO bioavailability. Inhalation of NO is nontoxic and at high concentrations (80 ppm) inhibits IR injury in extrapulmonary tissues. In this prospective, blinded, placebo-controlled study, we evaluated the hypothesis that administration of inhaled NO (iNO; 80 ppm) to patients undergoing orthotopic liver transplantation inhibits hepatic IR injury, resulting in improved liver function. Patients were randomized to receive either placebo or iNO (n = 10 per group) during the operative period only. When results were adjusted for cold ischemia time and sex, iNO significantly decreased hospital length of stay, and evaluation of serum transaminases (alanine transaminase, aspartate aminotransferase) and coagulation times (prothrombin time, partial thromboplastin time) indicated that iNO improved the rate at which liver function was restored after transplantation. iNO did not significantly affect changes in inflammatory markers in liver tissue 1 hour after reperfusion but significantly lowered hepatocyte apoptosis. Evaluation of circulating NO metabolites indicated that the most likely candidate transducer of extrapulmonary effects of iNO was nitrite. In summary, this study supports the clinical use of iNO as an extrapulmonary therapeutic to improve organ function following transplantation.
Nature Medicine | 2008
T. Scott Isbell; Chiao Wang Sun; Li Chen Wu; Xinjun Teng; Dario A. Vitturi; Billy Glynn Branch; Christopher G. Kevil; Ning Peng; Jm Wyss; Namasivayam Ambalavanan; Lisa M. Schwiebert; Jinxiang Ren; Kevin M. Pawlik; Matthew B. Renfrow; Rakesh P. Patel; Tim M. Townes
The coupling of hemoglobin sensing of physiological oxygen gradients to stimulation of nitric oxide (NO) bioactivity is an established principle of hypoxic blood flow. One mechanism proposed to explain this oxygen-sensing–NO bioactivity linkage postulates an essential role for the conserved Cys93 residue of the hemoglobin β-chain (βCys93) and, specifically, for S-nitrosation of βCys93 to form S-nitrosohemoglobin (SNO-Hb). The SNO-Hb hypothesis, which conceptually links hemoglobin and NO biology, has been debated intensely in recent years. This debate has precluded a consensus on physiological mechanisms and on assessment of the potential role of SNO-Hb in pathology. Here we describe new mouse models that exclusively express either human wild-type hemoglobin or human hemoglobin in which the βCys93 residue is replaced with alanine to assess the role of SNO-Hb in red blood cell–mediated hypoxic vasodilation. Substitution of this residue, precluding hemoglobin S-nitrosation, did not change total red blood cell S-nitrosothiol abundance but did shift S-nitrosothiol distribution to lower molecular weight species, consistent with the loss of SNO-Hb. Loss of βCys93 resulted in no deficits in systemic or pulmonary hemodynamics under basal conditions and, notably, did not affect isolated red blood cell–dependent hypoxic vasodilation. These results demonstrate that SNO-Hb is not essential for the physiologic coupling of erythrocyte deoxygenation with increased NO bioactivity in vivo.
Methods in Enzymology | 2008
Xinjun Teng; T. Scott Isbell; Jack H. Crawford; Charles A. Bosworth; Gregory I. Giles; Jeffrey R. Koenitzer; Jack R. Lancaster; Jeannette E. Doeller; David W. Kraus; Rakesh P. Patel
Recent advances in techniques that allow sensitive and specific measurement of S-nitrosothiols (RSNOs) have provided evidence for a role for these compounds in various aspects of nitric oxide (NO) biology. The most widely used approach is to couple reaction chemistry that selectively reduces RSNOs by one electron to produce NO, with the sensitive detection of the latter under anaerobic conditions using ozone based chemiluminescence in NO analyzers. Herein, we report a novel reaction that is readily adaptable for commercial NO analyzers that utilizes hydrogen sulfide (H2S), a gas that can reduce RSNO to NO and, analogous to NO, is produced by endogenous metabolism and has effects on diverse biological functions. We discuss factors that affect H2S based methods for RSNO measurement and discuss the potential of H2S as an experimental tool to measure RSNO.
Methods in Enzymology | 2005
T. Scott Isbell; Jeffrey R. Koenitzer; Jack H. Crawford; C.R. White; David W. Kraus; Rakesh P. Patel
Results from vessel bioassays have provided the foundation for much of our understanding of the mechanisms that control vascular homeostasis and blood flow. The seminal observations that led to the discovery that nitric oxide (NO) is a critical mediator of vascular relaxation were made with the use of such methodology, and many studies have used NO-dependent vessel relaxation as an experimental readout for understanding mechanisms that regulate vascular NO function. Studies have coupled controlling oxygen tensions within vessel bioassay chambers to begin to understand how oxygen-specifically hypoxia-regulate NO function, and this context has identified red cells-specifically hemoglobin within-as critical modulators. Alone, vessel bioassays or measuring oxygen partial pressures (pO2) is relatively straightforward, but the combination necessitates consideration of several factors. We use the example of deoxygenated red cells/hemoglobin-dependent potentiation of nitrite-dependent dilation to illustrate the salient factors that are critical to consider in designing and interpreting experiments aimed at understanding the interplay between oxygen and NO function in the vasculature.
Blood | 2006
Jack H. Crawford; T. Scott Isbell; Zhi Huang; Sruti Shiva; Balu K. Chacko; Alan N. Schechter; Victor M. Darley-Usmar; Jeffrey D. Kerby; John D. Lang; David W. Kraus; Chien Ho; Mark T. Gladwin; Rakesh P. Patel
Analytical Biochemistry | 2005
Jeannette E. Doeller; T. Scott Isbell; Gloria A. Benavides; Jeffrey R. Koenitzer; Hetal D. Patel; Rakesh P. Patel; Jack R. Lancaster; Victor M. Darley-Usmar; David W. Kraus
American Journal of Physiology-heart and Circulatory Physiology | 2007
Jeffrey R. Koenitzer; T. Scott Isbell; Hetal D. Patel; Gloria A. Benavides; Dale A. Dickinson; Rakesh P. Patel; Victor M. Darley-Usmar; Jack R. Lancaster; Jeannette E. Doeller; David W. Kraus
Journal of the American Chemical Society | 2006
Xin Sha; T. Scott Isbell; Rakesh P. Patel; and Cynthia S. Day; S. Bruce King
American Journal of Physiology-heart and Circulatory Physiology | 2007
T. Scott Isbell; Mark T. Gladwin; Rakesh P. Patel