Takaaki Kusumi
University of Tokyo
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Featured researches published by Takaaki Kusumi.
Virus Genes | 1994
Kazuyuki Ohira; Takao Ito; Akira Kawai; Shigetou Namba; Takaaki Kusumi; Tsuneo Tsuchizaki
The 3′-terminal sequence of citrus tatter leaf virus lily isolate (CTLV-L) was determined from cloned cDNA. The sequence contains two open reading frames (ORFs). ORF1 encodes a protein that contains consensus sequences associated with the RNA-dependent RNA polymerase. ORF2, which is in a different reading frame within ORF1, can encode a 36 kD protein, putatively identified as a movement protein. CTLV-L coat protein (CP) was found to be located in the C-terminal region of the polyprotein encoded by ORF1. Evolutionary relationships and classification of capilloviruses is discussed.
Virus Genes | 1994
Kazuyuki Ohira; Shigetou Namba; Masamichi Miyagawa; Takaaki Kusumi; Tsuneo Tsuchizaki
AbstractcDNA of tulip breaking virus-tulip (TBV-tulip) RNA was synthesized and cloned inE. coli. One clone that contains a 4.5 kb insert was identified by restriction enzyme analysis, dot immunobinding assay (DIBA), and partial sequencing. Then 1479 nucleotides of the 3′-terminus of the clone were sequenced and revealed that the sequence contains one open reading frame (ORF), followed by an untranslated region of 255 nucleotides and a poly(A) tract. The deduced amino acid sequence was found to include the C terminus of the predicted RNA-dependent RNA polymerase and the coat protein. A glutamine-alanine dipeptide was identified as a putative NIa protease cleavage site at the N terminus of the coat protein.
Archive | 1999
Shinzo Tsuda; Ken-ichi Suzuki; Hui-min Xue; Yoshikazu Tanaka; Yuko Fukui; Masako Fukuchi-Mizutani; Yukihisa Katsumoto; Takaaki Kusumi
Molecular breeding is a powerful method of plant breeding because it can change a specific characteristic of a plant without changing other desirable characteristics. Flower color is predominantly influenced by two types of pigments; flavonoids and carotenoids. The anthocyanin biosynthetic pathways of many plants have been well established (Holton et al, 1995) and conserved (Fig. 1). Chalcone synthase (CHS) and dihydroflavonol 4-reductase (DFR) are the first specific enzymes in flavonoid and anthocyanin biosynthesis, respectively. The presence of flavonoid 3′5′-hydroxylase (F3′5′H), cytochrome P-450 (Holton et al, 1993), is almost critical to the production of blue to purple anthocyanins. The flower color is reddish in its absence.
Plant and Cell Physiology | 2007
Yukihisa Katsumoto; Masako Fukuchi-Mizutani; Yuko Fukui; Filippa Brugliera; Timothy A Holton; Mirko Karan; Noriko Nakamura; Keiko Yonekura-Sakakibara; Junichi Togami; Alix Pigeaire; Guo-Qing Tao; Narender S. Nehra; Chin-Yi Lu; Barry K. Dyson; Shinzo Tsuda; Toshihiko Ashikari; Takaaki Kusumi; John Mason; Yoshikazu Tanaka
Plant Biotechnology | 2004
Shinzo Tsuda; Yuko Fukui; Noriko Nakamura; Yukihisa Katsumoto; Keiko Yonekura-Sakakibara; Masako Fukuchi-Mizutani; Kazuyuki Ohira; Yukiko Ueyama; Hideo Ohkawa; Timothy A. Holton; Takaaki Kusumi; Yoshikazu Tanaka
Journal of General Virology | 1995
Kazuyuki Ohira; Shigetou Namba; Michael Rozanov; Takaaki Kusumi; Tsuneo Tsuchizaki
Plant Biotechnology | 2008
Takeshi Kanaya; Hiroyuki Saito; Yoriko Hayashi; Nobuhisa Fukunishi; Hiromichi Ryuto; Kiyoshi Miyazaki; Takaaki Kusumi; Tomoko Abe; Ken-ichi Suzuki
Plant Biotechnology | 2006
Junichi Togami; Mihoko Tamura; Kanako Ishiguro; Chika Hirose; Hiroaki Okuhara; Yukiko Ueyama; Noriko Nakamura; Keiko Yonekura-Sakakibara; Masako Fukuchi-Mizutani; Ken-ichi Suzuki; Yuko Fukui; Takaaki Kusumi; Yoshikazu Tanaka
Tetrahedron | 2006
Yuko Fukui; Kyosuke Nomoto; Takashi Iwashita; Katsuyoshi Masuda; Yoshikazu Tanaka; Takaaki Kusumi
Archive | 1996
Toshihiko Ashikari; Yoshikazu Tanaka; Hiroyuki Fujiwara; Masahiro Nakao; Yuko Fukui; Keiko Sakakibara; Masako Mizutani; Takaaki Kusumi