Takaro Esaki

An antigen specific to hyperplastic liver nodules defined with monoclonal antibody: a new marker for preneoplastic cells in rat chemical hepatocarcinogenesis.

Two monoclonal antibodies (MoAbs) were prepared against the hepatocytes of hyperplastic liver nodules from rats killed in the 13th week during hepatocarcinogenesis with 2-acetylaminofluorene. The specificity of these MoAbs was confirmed by cellular radioimmunoassay. By means of immunoperoxidase staining with 2 MoAbs (HAM 6 and HAM 7), HAM 6-defined antigen was detected on the plasma membrane and/or cytoplasm of the hepatocytes in hyperplastic nodules, while HAM 7 reacted to the hepatocytes surrounding hyperplastic nodules. Therefore, HAM 6 seemed to be specific to hyperplastic nodules, but HAM 7 to be a MoAb developed accidentally to the surrounding hepatocytes by contamination of the cell suspension of hyperplastic nodules.

Effects of calcium-ion on biliary excretion of loaded bile acid and ICG

カルシウム(Ca)を前投与したラットについて,タウロコール酸(TCA),インドシアニングリーン(ICG)を静注し,胆汁中への排泄におよぼすCaの影響を調べた.まず,Ca投与後の胆汁および胆汁中胆汁酸の基礎排泄量を測定し,TCA(1.4μmol/100g体重)負荷による変化をさらに検索した.胆汁中胆汁酸は酵素法および高速液体クロマトグラフィーで測定した.ICGはそれぞれ0.5, 1.5, 3.0mg/100g体重量を静注し,胆汁への排泄および5, 10, 20分目の血中ICG値の変化を認べた.その結果,基礎排泄量には変化はみられなかったが,TCA投与後の胆汁量,胆汁中TCA排泄量はともに対照群に比して有意に上昇し,ICGの胆汁への排泄もそれぞれの群で有意に亢進した.一方,ICGの血中消失には差はなかった.これにより,負荷胆汁酸およびICGの胆汁への排泄は,Caの投与で亢進することが明らかになり,毛細胆管でのexocytosisにもCaの影響が示唆された.