Takashi S. Matsuda

Distinct neural mechanisms for the control of thirst and salt appetite in the subfornical organ

Body fluid conditions are continuously monitored in the brain to regulate thirst and salt-appetite sensations. Angiotensin II drives both thirst and salt appetite; however, the neural mechanisms underlying selective water- and/or salt-intake behaviors remain unknown. Using optogenetics, we show that thirst and salt appetite are driven by distinct groups of angiotensin II receptor type 1a-positive excitatory neurons in the subfornical organ. Neurons projecting to the organum vasculosum lamina terminalis control water intake, while those projecting to the ventral part of the bed nucleus of the stria terminalis control salt intake. Thirst-driving neurons are suppressed under sodium-depleted conditions through cholecystokinin-mediated activation of GABAergic neurons. In contrast, the salt appetite-driving neurons were suppressed under dehydrated conditions through activation of another population of GABAergic neurons by Nax signals. These distinct mechanisms in the subfornical organ may underlie the selective intakes of water and/or salt and may contribute to body fluid homeostasis.

New statistical analysis of the horizontal phase velocity distribution of gravity waves observed by airglow imaging

We have developed a new analysis method for obtaining the power spectrum in the horizontal phase velocity domain from airglow intensity image data to study atmospheric gravity waves. This method can deal with extensive amounts of imaging data obtained on different years and at various observation sites without bias caused by different event extraction criteria for the person processing the data. The new method was applied to sodium airglow data obtained in 2011 at Syowa Station (69°S, 40°E), Antarctica. The results were compared with those obtained from a conventional event analysis in which the phase fronts were traced manually in order to estimate horizontal characteristics, such as wavelengths, phase velocities, and wave periods. The horizontal phase velocity of each wave event in the airglow images corresponded closely to a peak in the spectrum. The statistical results of spectral analysis showed an eastward offset of the horizontal phase velocity distribution. This could be interpreted as the existence of wave sources around the stratospheric eastward jet. Similar zonal anisotropy was also seen in the horizontal phase velocity distribution of the gravity waves by the event analysis. Both methods produce similar statistical results about directionality of atmospheric gravity waves. Galactic contamination of the spectrum was examined by calculating the apparent velocity of the stars and found to be limited for phase speeds lower than 30 m/s. In conclusion, our new method is suitable for deriving the horizontal phase velocity characteristics of atmospheric gravity waves from an extensive amount of imaging data.

Detection of LacZ-Positive Cells in Living Tissue with Single-Cell Resolution

The LacZ gene, which encodes Escherichia coli β-galactosidase, is widely used as a marker for cells with targeted gene expression or disruption. However, it has been difficult to detect lacZ-positive cells in living organisms or tissues at single-cell resolution, limiting the utility of existing lacZ reporters. Herein we present a newly developed fluorogenic β-galactosidase substrate suitable for labeling live cells in culture, as well as in living tissues. This precisely functionalized fluorescent probe exhibited dramatic activation of fluorescence upon reaction with the enzyme, remained inside cells by anchoring itself to intracellular proteins, and provided single-cell resolution. Neurons labeled with this probe preserved spontaneous firing, which was enhanced by application of ligands of receptors expressed in the cells, suggesting that this probe would be applicable to investigate functions of targeted cells in living tissues and organisms.

Characteristics of mesospheric gravity waves over Antarctica observed by Antarctic Gravity Wave Instrument Network imagers using 3-D spectral analyses

We have obtained horizontal phase velocity distributions of the gravity waves around 90 km from four Antarctic airglow imagers, which belong to an international airglow imager/instrument network known as ANGWIN (Antarctic Gravity Wave Instrument Network). Results from the airglow imagers at Syowa (69°S, 40°E), Halley (76°S, 27°W), Davis (69°S, 78°E) and McMurdo (78°S, 167°E) were compared, using a new statistical analysis method based on 3-D Fourier transform [Matsuda et al., 2014] for the observation period between 7 April and 21 May 2013. Significant day-to-day and site-to-site differences were found. The averaged phase velocity spectrum during the observation period showed preferential westward direction at Syowa, McMurdo and Halley, but no preferential direction at Davis. Gravity wave energy estimated by I’/I was ~5 times larger at Davis and Syowa than at McMurdo and Halley. We also compared the phase velocity spectrum at Syowa and Davis with the background wind field and found that the directionality only over Syowa could be explained by critical level filtering of the waves. This suggests that the eastward propagating gravity waves over Davis could have been generated above the polar night jet. Comparison of nighttime variations of the phase velocity spectra with background wind measurements suggested that the effect of critical level filtering could not explain the temporal variation of gravity wave directionality well, and other reasons such as variation of wave sources should be taken into account. Directionality was determined to be dependent on the gravity wave periods.