Tati Herlina

Chemical constituents of Piper aduncum and their inhibitory effects on soluble epoxide hydrolase and tyrosinase

A new compound, 2(S)-isobutanol 2-O-β-d-arabinopyranosyl(1→6)-O-β-d-glucopyranoside (1), along with ten known compounds (2–11) were isolated from Piper aduncum L. leaves. The effects of these compounds on soluble epoxide hydrolase and tyrosinase inhibition were evaluated. Among them, compounds 3, 8, and 9 exhibited significant tyrosinase inhibitory activity with IC50 values of 39.3 ± 1.8, 41.3 ± 2.2, and 37.5 ± 2.7 µM, respectively. However, the effects of isolated compounds on soluble epoxide hydrolase inhibition were weak or absent, and compounds 4 and 11 showed the highest inhibitory activity with values of 61.2 ± 4.3 and 60.6 ± 3.7 % at a concentration of 100 μM.

Dataset of curcumin derivatives for QSAR modeling of anti cancer against P388 cell line.

The dataset of curcumin derivatives consists of 45 compounds (Table 1) with their anti cancer biological activity (IC50) against P388 cell line. 45 curcumin derivatives were used in the model development where 30 of these compounds were in the training set and the remaining 15 compounds were in the test set. The development of the QSAR model involved the use of the multiple linear regression analysis (MLRA) method. Based on the method, r2 value, r2(CV) value of 0.81, 0.67 were obtained. The QSAR model was also employed to predict the biological activity of compounds in the test set. Predictive correlation coefficient r2 values of 0.88 were obtained for the test set.

Flavonoids from the Fresh Leaves of Kalanchoe tomentosa (Crassulaceae)

Flavonoids compounds such as kaempferol (1), kaempferol-3-O- -D-glucopyranoside or astragalin (2) and kaempferol-3-O- -L-rhamnoside or afzelin (3) have been isolated from the fresh leaves of Kalanchoe tomentosa (Crassulaceae). The chemical structure of isolated compounds 1-3 were identified by spectroscopic evidences and comparison with those compound previously reported. Compounds 1-3 showed cytotoxic activity against P-388 murine leukimia cells with IC50 values of 51.8, � 100 and 3.32 μg/mL, respectively.

3,5-Bis(2-hydroxybenzylidene)piperidin-4-one

The title compound, 3,5-bis(2-hydroxybenzylidene)piperidin-4-one (3), was prepared via reaction of 2-hydroxybenzaldehyde (1) and 4-piperidone (2) under microwave irradiation in the presence of 10% NaOH solution. The compound was fully characterized from its UV, IR, NMR and MS data.

MANOVA Statistical Analysis of Inorganic Compounds in Groundwater Indonesia

The present study was carried out to determine levels of inorganic compounds contained in the ground water and Reverse Osmosis (RO) water filtration result. The data in groundwater samples was collected from Bekasi, Tangerang and Jakarta in Indonesia. A total of 30 samples were collected and analyzed for the determine Cadmium (Cd), Chromium (Cr), Manganese (Mn), Cyanide (CN) and Lead (Pb). The results of the study revealed that in groundwater, the average of Cd 0.0058 mg / l, Mn 1.5233 mg / l, Cr 0.0127 mg/l, Pb 0.0060 mg / l, and CN 0.0040 mg / l. The level of RO result were: Cd 0.0027 mg / l, Mn 0.1767 mg / l, Cr 0.0024 mg / l, Pb 0.0021 mg / l, and CN 0.0023 mg / l . This means that Cd and Mn in ground water were higher than the values recommended by PAK-EPA and WHO or the standard of Indonesian Ministry of Health. But after filtration Reverse Osmosis (RO) Mn and Cd levels decreased to levels below the standardized value. By comparing of mean in MANOVA and nonparametric MANOVA in α=5%, there are differ...

A new limonoid from stem bark of Chisocheton pentandrus (Meliaceae)

Abstract A new limonoid, pentandricine (1), along with three known limonoids, ceramicine B (2), 6-de(acetyloxy)-23-oxochisocheton (3), 6-de(acetyloxy)-23-oxo-7-O-deacetylchisocheton (4), have been isolated from the stembark of Chisocheton pentandrus. The chemical structures of the new compound were elucidated on the basis of spectroscopic evidence. All of the compounds were tested for their cytotoxic effects against MCF-7 breast cancer cells. Compounds 1–4 showed weak and no cytotoxicity against MCF-7 breast cancer cells with IC50 values of 369.84, 150.86, 208.93 and 120.09 μM, respectively.

POTENCY OF ANTIOXIDANT, ANTICHOLESTEROL AND PLATELET ANTIAGGREGATION OF BLACK TEA (Camelia sinensis )

Kendala dalam penyediaan benih adalah ketersediaan yang tepat waktu, tepat jum-lah, seragam dan sehat. Teknik kultur ja-ringan dapat memecahkan kendala terse-but tetapi biayanya cukup tinggi sehingga harga benih menjadi mahal 3-4 kali harga benih konvensional. Untuk mengatasi hal tersebut pada tanaman nilam, dilakukan perbanyakan benih secara kultur jaringan dengan mensubstitusi bahan kimia yang harganya mahal dengan bahan-bahan al-ternatif yang mudah diperoleh seperti air kelapa dan sumber bahan organik lainnya. Penelitian dilaksanakan di laboratorium kultur jaringan, laboratorium Pengujian Balittro, Balai Besar Pasca Panen, dan ru-mah kaca Balittro sejak Mei 2009 sampai Oktober 2010. Penentuan harga pokok dan skala ekonomi dilakukan secara bertahap : (1) harga pokok zat pengatur tumbuh (zpt) alternatif, terdiri dari air kelapa, ekstrak to-mat, dan ekstrak tauge, (2) harga pokok tunas hasil induksi dari eksplan varietas Sidikalang dengan media Murashige dan Skoog (MS) ditambah zpt alternatif dan sumber vitamin substitusi dari air kelapa, tomat, tauge, dan wood vinegar masing-masing dengan konsentrasi 0 (kontrol), 5, 10, 15, 20, dan 25%, (3) harga pokok multiplikasi tunas nilam dengan media ter-baik pada tahap induksi, (4) harga pokok tunas hasil multiplikasi media MS + air ke-lapa konsentrasi 10% dibandingkan de-ngan media dasar alternatif pupuk maje-muk dengan formulasi NPK 20-20-20 yaitu: (a) pupuk majemuk 0,5 1 g/l + air kelapa 10%, (b) pupuk majemuk 1 g/l + air kela-pa 10%, (c) pupuk majemuk 1,5 g/l + air kelapa 10%, (d) pupuk majemuk 2 g/l + air kelapa 10%, (e) MS + BA 0,5 mg/l, (f) MS + air kelapa 10%, (5) harga pokok ni-lam hasil aklimatisasi di rumah kaca de-ngan perlakuan beberapa jenis media : (a) tanah latosol (kontrol), (b) tanah lato-sol + kompos serasah tanaman (1:1), (c) tanah latosol + arang/sekam padi (1:1), (d) tanah latosol + cocopeat (1:1), (e) ta-nah latosol + kompos serasah tanaman + arang/sekam padi (1:1:1), (f) tanah lato-sol + kompos serasah tanaman + coco-peat (1:1:1), (g) tanah latosol + kompos serasah tanaman + arang/sekam padi + cocopeat (1:1:1:1), (6) harga pokok dan skala usaha nilam di dalam polybag ukur-an 10 x 15 cm dengan media tanam ta-nah + pupuk kandang (2:1). Penentuan harga pokok benih nilam dan skala usaha-nya, dilakukan dengan menganalisis input dan out-put kegiatan produksi benih ni-lam hasil kultur jaringan. Hasil penelitian menunjukkan harga pokok benih nilam skala laboratorium adalah Rp339 per tu-nas dengan media perbanyakan MS di-tambah zpt alternatif air kelapa konsen-trasi 10%, atau Rp796/polybag dengan titik impas/ break event point pada jumlah produksi 51.415 polybag benih per 3,5 bulan setelah aklimatisasi, setara dengan pendapatan sebesar Rp40.926.258.Potency of Medicinal and Aromatic Crop Extracts to control Golden Snail Research aimed to evaluate potency of some medicinal and aromatic plants as raw materials of molluscicides to control the golden snail has been done in the En-tomological Laboratory of Medicinal and Aromatic Crops Research Institute, Bogor, Indonesia from February to October 2008. The study used factorial ith rando-mized block design basis. The first factor is three levels dipping time of 5, 10, and 20 hours. The second factor is 14 kinds of extracts to be tested i.e. extracts of clove, the crown of god, seraiwangi, turmeric, physic nut, pig nut, legundi, chili java, ba-badotan, brotowali, bitter, kenikir, noni, and rerak. Research initially conducted by dissolving 5% tween 80 in water. After that into the 1 l of solution is placed 5 g of extract to be tested so that the concen-tration of extract in the solution to be tested is 0.5%. After that the snails were put into the solution and were fed with ta-ro leaf ( Colocasia giganteum ). In the con-trol treatment the snail only be soaked in water containing 5% tween 80. Soaking performed for 5, 10, and 20 hours in glass jars 9 x 15 cm 2 diameter. After soaking snails then were transferred into the bottle containing clean water and then given a taro leaf size ~100 cm 2 as food. Each tre-atment used 10 snails and repeated 3 times. Observations were made at 1, 2, and 3 days after application of the golden snail mortality by counting the number of snails that died after treatment and of inhibition of eating by noting the eaten leaf area. The results showed that the clove, the crown of god, Cintronella oil, and tur-meric are the most effective extract with 100% mortality rate and percentage of >90% inhibition of eating. Among the plants tested, clove most prospective to be developed as a controlling golden snail due to its high yield and the most immediate cause of death of the snail test.Penelitian pengaruh penyakit budok yang disebabkan oleh jamur obligat parasit Synchytrium pogostemonis ter-hadap produksi tanaman nilam ( Pogos-temon cablin ) dilakukan di daerah en-demik penyakit budok di Nagari Situak, Kabupaten Pasaman Barat, Sumatera Barat sejak Maret 2008 sampai April 2009. Bahan tanaman yang digunakan adalah varietas Sidikalang, berumur satu bulan ditanam dalam polibag di lapang dalam tiga kelompok. Masing-masing kelompok terdiri dari 120 bibit dengan jarak tanam 0,8 x 1 m. Pe-ngamatan gejala serangan dilakukan setiap bulan dengan metode sensus. Intensitas serangan patogen penyakit dibagi menjadi lima kategori berdasar-kan nilai skoring yaitu tanaman sehat dan tanaman sakit yang terdiri dari gejala serangan ringan, sedang, berat dan sangat berat. Setelah tanaman berumur enam bulan, 15 rumpun un-tuk tiap kategori intensitas serangan dipilih dengan metode proposive sam-pling . Bahan tanaman untuk disuling diambil sebanyak 20 kg dari pada ma-sing-masing kategori intensitas serang-an penyakit. Penyulingan mengguna-kan sistim kukus selama 3 jam. Analisa kadar patchouli alcohol (PA) dilakukan di laboratorium Perusahaan Atsiri Lu-buk Minturun Padang. Hasil penelitian menunjukan bahwa serangan penyakit budok menurunkan produksi terna dan kadar minyak nilam, tetapi tidak menurunkan kualitas minyak yang di-hasilkan. Intensitas serangan penyakit budok ringan menyebabkan kehilang-an hasil produksi terna sebesar 16,74 %, intensitas serangan sedang 36,06 %, intensitas serangan berat 57,43% dan intensitas penyakit sangat berat sebesar 74,32%. Kehilangan produksi minyak pada intensitas serangan ri-ngan adalah 11,15%, intensitas se-rangan sedang 18,32%, intensitas se-rangan berat 35,50% dan intensitas serangan sangat berat sebesar 50,38 %. Hasil analisa PA pada berbagai tingkat intensitas penyakit budok ti-dak berbeda nyata. Kadar PA pada ta-naman sehat sebesar 32,84%, dan tanaman sakit berkisar antara 32,15-33,43%.

N-benzyl-(3E,5E)-3,5-bis(2-hydroxybenzylidene)-4-piperidone

A novel N-benzyl-(3E,5E)-3,5-bis(2-hydroxybenzylidene)-4-piperidone (3), was synthesized in good yield by a condensation reaction of 2-hydroxybenzaldehyde (1) and N-benzyl-4-piperidone (2) under microwave irradiation in the presence of 10% NaOH solution. The chemical structure was assigned on the basis of UV-visible, IR, 1H-NMR, 13C-NMR and mass spectral data.

Crystal structures of 3,5-bis-[(E)-3-hy-droxy-benzyl-idene]-1-methyl-piperidin-4-one and 3,5-bis-[(E)-2-chloro-benzyl-idene]-1-methyl-piperidin-4-one.

3,5-Bis[(E)-3-hydroxybenzylidene]-1-methylpiperidin-4-one and the 2-chlorobenzylidene derivative are monocarbonyl analogues of curcumin, and the conformations of the two compounds are very similar. In the 3-hydroxybenzylidene compound, O—H⋯O, O—H⋯N and C—H⋯O hydrogen bonds link the molecules, forming sheets lying parallel to the ac plane. In the crystal of the 2-chlorobenzylidene derivative, molecules are linked via weak C—H⋯Cl hydrogen bonds, forming zigzag chains along the [204] direction.

Spatial Autocorrelation of Inorganic Compound in Groundwater

The water quality at a location is influenced other locations. Characteristics of groundwater quality in some locations were spatially auto correlated. Such as in Jakarta, Indonesia, if the water source in one location is polluted so can lead to polluted water sources at a different location which close together. This research was analyzing the content of inorganic compounds in Jakarta, Indonesia. The analysis was performed using spatial methods to obtain the relationship among locations of ground water quality. The samples were ten locations in East, West, Central, North, and South Jakarta. Spatial methods conclude that there were spatial autocorrelation in inorganic compounds of groundwater in Jakarta, specifically in Cadmium (Cd). Moran’s I test shows that there are dependencies of Cd among 10 samples at α = 10%. Meanwhile, Mn, CN, Cr, and Pb haven’t dependencies. By LISA test, there were the first sample in West Jakarta and seventh sample in East Jakarta which significant affects with other location at α = 5%. P value in area west, north, and east of Jakarta were lower than others. So, the samples in these areas have dependencies than in south area.