Tatiana Amaral Guerra

Prevalência dos tipos sangüíneos A, B e AB em gatos domésticos mestiços da cidade de Porto Alegre, Rio Grande do Sul, Brasil

O presente estudo teve como objetivo determinar a prevalencia dos tipos sanguineos em felinos domesticos, mesticos, da cidade de Porto Alegre, Rio Grande do Sul, Brasil. Foram selecionados aleatoriamente 100 gatos, clinicamente saudaveis, mesticos e sem parentesco entre si. Amostras de sangue foram coletadas da veia jugular e a tipagem sanguinea foi realizada atraves do teste RapidVet H Feline (DMS Laboratories, Flemington, USA) e do teste hemaglutinacao em tubo de ensaio. A tipagem reversa foi realizada para confirmar o tipo e a presenca de aloanticorpos naturais. No presente estudo encontrou-se prevalencia de 97% e 3% de gatos do tipo A e B, respectivamente. Nao foram encontrados gatos do tipo AB. Os resultados indicam que no sul do Brasil ha uma alta prevalencia de gatos domesticos mesticos do tipo A, entretanto a prevalencia de gatos do tipo B encontrada no trabalho e mais alta do que aquelas relatadas em alguns paises. O conhecimento da prevalencia dos tipos sanguineos da populacao de gatos de uma regiao pode auxiliar na determinacao dos riscos de reacoes transfusionais e de ocorrencia de isoeritrolise neonatal, e estes podem ser prevenidos atraves de tipagem sanguinea e teste de compatibilidade sanguinea.

Titulação de aloanticorpos anti-a e anti-b em gatos domésticos sem raça definida em Porto Alegre, Rio Grande do Sul, Brasil

The probability of occurrence of a transfusion reaction in a cat depends on the local prevalence of feline blood typesand alloantibody titres. Determination of alloantibody titres assists in estimating the risk and the severity of transfusionreactions following an unmatched transfusion in a cat population. The objective of this study was to determine alloantibodytitres and the risk of a possible transfusion reaction in non-pedigree domestic cats in Porto Alegre, Brazil. One hundredclinically healthy non-pedigree and non-related cats with no history of previous transfusion were randomly selected.Blood samples were collected from the jugular vein and the alloantibody titres were determined by tube serial dilution ofthe serum from cats with known blood types, the estimated risk was calculated according previous studies. In the presentstudy, 82.5% and 100% of types A and B cats, respectively, showed varied alloantibodies titres. Based on the titres foundin the present study, the risk of an acute severe reaction is 33.3% and an acute mild reaction is 66.7% in a type B cattransfused with blood type A or AB. In type A cats transfused with blood type AB or B, the risk of an acute severe reactionis 1.0%, an acute mild reaction is 37.1% and premature red cell destruction is 44.3%.

Evaluation of tumor markers carcinoembryonic antigen, cytokeratin 19 fragment and cancer-associated antigen 72-4 in neoplastic and non-neoplastic canine effusions differentiation

The concentration of tumor markers in body fluids can be used for diagnosis and prognosis of patients. This study aimed to investigate the performance of tumor markers cytokeratin 19 fragment (CYFRA 21-1), cancer-associated antigen 72-4 (CA 72-4) and carcinoembryonic antigen (CEA) in the neoplastic and non-neoplastic canine effusions. In thirty-two neoplastic (n=16) and non-neoplastic (n=16) samples of canine thoracic or abdominal effusions, tumor markers were measured. Significant statistical difference was found only for the CYFRA 21-1 marker. The levels were significantly higher for the neoplastic group. The lack of significance between groups for markers CA 72-4 and CEA can be explained by the presence of other diseases in the non-neoplastic group, causing elevated levels of these markers. This study concludes that CYFRA 21-1 performed well, showing good sensitivity, specificity and accuracy in the diagnosis of neoplastic effusions in dogs. However, further investigations are necessary in patients with malignancy as those with benign effusions.