Tatiana Nayara Libório

In situ hybridization detection of homeobox genes reveals distinct expression patterns in oral squamous cell carcinomas.

Libório T N, Acquafreda T, Matizonkas‐Antonio L F, Silva‐Valenzuela M G, Ferraz A R & Nunes F Du2028(2011) Histopathology 58, 225–233u2028In situ hybridization detection of homeobox genes reveals distinct expression patterns in oral squamous cell carcinomas

Evaluation of the genomic DNA extracted from formalin-fixed, paraffin-embedded oral samples archived for the past 40-years

INTRODUCAO: O material fixado em formol e embebido em parafina constitui hoje a maior fonte de tecido humano arquivado. O DNA extraido de tecido parafinado por vezes nao e adequado para as tecnicas de biologia molecular, visto que se apresenta parcialmente degradado. OBJETIVOS: O objetivo desse estudo foi verificar se tecido humano de boca parafinado e arquivado pelos ultimos 40 anos pode ser usado para a reacao em cadeia da polimerase (PCR). METODOS: Amostras foram submetidas a tecnica de extracao de DNA pelo metodo do fenol-cloroformio. Para quantificacao e qualificacao do DNA foram realizadas analise em espectrofotometro, eletroforese em gel de agarose e amplificacao pela tecnica da PCR. RESULTADOS: Foi observada fraca correlacao positiva entre a quantidade de DNA obtida e a idade das amostras. A eletroforese em gel de agarose demonstrou que a maioria do DNA obtido foi constituida de fragmentos pequenos. O fragmento de 268-pb do gene da b-globina foi amplificado em 55% dos casos, preferencialmente nos casos mais recentes. O fragmento de 149-pb do gene WAF1 apresentou amplificacao fraca, mas presente em 75% dos casos. O fragmento de 536-bp do gene da b-globina foi detectado em somente 25% dos casos e tambem preferencialmente nos casos mais recentes. CONCLUSOES: Esse estudo mostrou que, apesar de o DNA estar degradado, e possivel usar DNA genomico extraido de tecido parafinado arquivado pelos ultimos 40 anos em reacoes de PCR de produtos pequenos. A amplificacao de produtos maiores, entretanto, e mais dificil.

Asymptomatic expansile lesion in the nasolabial region of a 10-year-old child

A 10-year-old black female with a painless swelling inher left nasolabial region was referred to the surgeryclinic. She reported that she had noticed the swelling for 3months. Clinical examination showed facial asymmetrydue to expansion of the side of the nose. There was noalteration in color or temperature of the skin around theswelling (Fig. 1). During an intraoral clinical examination,palpation revealed a firm mass, and the swelling had thesame color as normal mucosa (Fig. 2). The mass seemedto be restricted to soft tissue, and it involved the alveolarmucosa from the left permanent central incisor to the firstleft primary molar. It measured up to 50 mm in diameter.Panoramic and lateral skull radiographs showed thatthe osseous portion was not involved. Ultrasonographyof the face revealed a hyperechoic lesion with hypo-echoic areas (Fig. 3), possibly indicating a cystic cavityor an abscess. Computerized tomography showed anonspecific image with a homogeneous expansive massin the left part of the maxilla involving the left nasalcavity and limited to soft tissue. The lesion crossed thefacial midline (Fig. 4). There was no evidence of re-gional lymphadenopathy. There was nothing in thepatient’s medical history that had any bearing on thediagnosis.

Detection of TGIF1 homeobox gene in oral squamous cell carcinoma according to histologic grading

OBJECTIVEnTGIF1 homeobox gene involvement in oral cancer has not yet been investigated. This study analyzed the expression of TGIF1 transcripts and protein in oral squamous cell carcinoma (OSCC).nnnSTUDY DESIGNnSnap-frozen samples from 16 patients were taken from both OSCC and nontumoral adjacent epithelium (NT) for in situ hybridization (ISH). Forty-six paraffin-embedded samples of OSCC were submitted to immunohistochemistry (IHC). A descriptive analysis of the transcript signal detection was accomplished, and TGIF1 immunoexpression was carried out considering protein levels, localization, and cellular differentiation.nnnRESULTSnISH reactions showed TGIF1 transcripts with a signal that was frequently intense in NT, and generally weak in OSCC, and that had stronger transcript signal in well-differentiated areas of OSCC when compared with poorly differentiated ones. IHC reactions had poorly differentiated cases associated with TGIF1 protein expression in both the nucleus and cytoplasm (P = .05, Fisher test).nnnCONCLUSIONSnTGIF1 gain or loss of function might possibly play a role in oral cancer cell differentiation.

Diagnosis and Intralesional Corticotherapy in Oral Ulcers Occurring as the Sole Manifestation of Langerhans Cell Histiocytosis. A Case Report

This article reports a case of oral mucosa lesions as the sole manifestation in Langerhans cell histiocytosis (LCH). This is a very uncommon manifestation of LCH since this disease preferably affects the bones with frequent involvement of the jaws. LCH may also involve other organs, particularly the lungs, liver, lymph nodes, and skin. The highlights of this report are the differential diagnosis, immunohistochemical analysis and, mostly, the therapeutic approach.