Terry McLendon

Nitrogen availability and old-field succession in a shortgrass steppe.

ABSTRACT The relationship between soil nitrogen (N) availability and plant community structure was investigated in old-fields in the shortgrass steppe of Colorado. Nitrogen availability was manipulated by N or sucrose additions for 4 years at three old-fields (early-seral, mid-seral, and late-seral) and at an uncultivated control site. The addition of N generally resulted in increased abundance of annual forbs and grasses relative to perennials at all of the previously cultivated sites. Conversely, experimental reduction of N availability generally increased the relative abundance of perennials. Despite a lack of detectable differences in N mineralization between sites and treatments, ion-exchange resin bags confirmed that sucrose additions reduced plant-available N and that N additions increased plant-available N. This was evidenced further by similar observations for plant tissue N content. The degree to which N additions increased N availability at the various sites supported the idea that late-seral plant communities are less effective at N capture relative to earlier-seral communities. The mid-seral old-field had the lowest rates of litter decomposition and a relatively large accumulation of litter on the soil surface. This mid-seral old-field was dominated by an exotic annual grass (Bromus tectorum), which appears to be a major hindrance to redevelopment of the plant-soil system. By experimentally reducing N availability at this stage, we were able, in 4 years, to change the plant community into one that more closely resembled the late-seral community. We also observed that the natural recruitment of weedy annual species on the uncultivated site during an unusually wet year was suppressed by reducing N availability. Our results suggest that available N is an important factor controlling the rate and course of plant and soil community redevelopment on abandoned croplands in the shortgrass steppe, and that manipulation of N availability might be useful in restoration of rangeland vegetation.

Effects of nitrogen limitation on species replacement dynamics during early secondary succession on a semiarid sagebrush site

SummaryA soil nitrogen (N) availability gradient was induced on a disturbed sagebrush site in northwestern Colorado by fertilizing with nitrogen (high available N), applying sucrose (low available N), and applying neither nitrogen nor sucrose (control). Species composition was studied for 3 years. At the end of the study, N concentration of aboveground tissue of 3 major species was determined. The rate of species replacement was most rapid on plots receiving the sucrose treatment and was slowest on plots receiving the N treatment. Early-seral dominats had greater tissue N concentrations when availability of the resource was high but lower tissue N concentrations when available soil N became limited. Midseral dominants displayed the opposite pattern. These results suggest that the supply of available soil N, and therefore the dynamics of N incorporation in perennial plant tissue, is a primary mechanism in controlling the rate of secondary succession within this semiarid ecosystem.

Nitrogen and Phosphorus Effects on Secondary Succession Dynamics on a Semi‐Arid Sagebrush Site

A sagebrush steppe community in northwestern Colorado was disturbed in 1984 and subjected to annual applications of nitrogen and phosphorus, and successional responses were studied over a 5-yr period. Phosphorus was not found to be significant but nitrogen did significantly affect succession for all years except the first. Three seral groups developed on the non-fertilized plots, the first two dominated by annuals and lasting 3 yr, the third transitional and dominated by perennials. The addition of N altered this successional pattern by allowing annuals to remain as site dominants through the 5th yr. Results of this study suggests that dominance of a site by annuals in early stages of secondary succession is related to high nutrient availability.

Response of early and late semiarid seral species to nitrogen and phosphorus gradients

Above- and below-ground biomass production, nitrogen (N) and phosphorus (P) tissue concentrations, and root: shoot ratios were examined for five species that are characteristic of a semiarid successional sequence under controlled greenhouse conditions. In two simultaneous experiments, seedlings of one forb, two grass, and two shrub species important in a sagebrush successional sere, were subjected to seven levels of N and P. Results of the experiments suggest distinct differences in nutrient response patterns between early and late seral species. Early seral species produced more biomass but had lower tissue nutrient concentrations than late seral species. As N and P availabilities decreased, late seral species displayed characteristics indicative of increasing competitive advantage over those of early seral species. Root: shoot ratios of the five species primarily reflected patterns related to lifeform, but with some early and late seral characteristics. Results from this study 1) confirm that nutrient use pattern, nutrient availability, and seral position relationships characteristic of mesic ecosystems hold equally true for semiarid systems, and 2) suggest that nutrients are important organizing factors in semiarid ecosystems.

Saprophytic fungal-bacterial biomass variations in successional communities of a semi-arid steppe ecosystem

A major goal in attempting to understand plant succession is to assess the implications of fungal and bacterial biomass changes over time as plant-soil systems develop. In this study, the soil fungal and bacterial biomass of three successional semi-arid steppe communities, sampled 4, 12, and 38 years after cultivation ended, were compared with an uncultivated native plant community using microscopic procedures. In the course of the succession, significant increases in fungal hyphal lengths occurred, reaching a maximum in the oldest successional (38-year) community. Active (cytoplasm filled) hyphae decreased along the chronosequence, with the native plant community having the lowest values. Similar decreases in active bacterial biomass values occurred. In contranst, microscopically determined total bacterial numbers did not differ in soils associated with the 4-year-old and native plant communities. The ratio of active bacterial to fungal biomass, which increased over the chronosequence tested in this study, appears to provide a valuable integrative measure of plant-soil resource system development and ecosystem maturity.

Succession patterns following soil disturbance in a sagebrush steppe community

SummaryA study was begun in 1976 to measure succession patterns following soil disturbance within a sagebrush community in northwestern Colorado. The principal hypothesis was that type of disturbance affects the direction of succession, resulting in different plant communities over time. Successional dynamics were studied through 1988. Four types of soil disturbance resulted in 3 early seral communities: one dominated by grasses, one by annuals, and one intermediate. The annual-dominated communities were opportunistic on these sites, lasting 3–5 years and not determining the direction in which succession proceeded following their replacement. Twelve years after disturbance, 3 communities (one grass-dominated, one shrub-dominated, and one intermediate) occupied the site, the characteristics of which were functions of type of initial soil disturbance. For the period of time covered by this study (12 years), degree of disturbance was found to affect the direction of succession, resulting in different plant communities over time. There were, however, successional characteristics toward the end of the study that suggest that over a longer time period, succession might progress to a single community regardless of type of disturbance.

Evidence of depleted uranium‐induced hormesis and differential plant response in three grasses

Abstract General plant response to depleted uranium (DU) was tested using three widespread range‐grass species, Aristida purpurea Nutt. (purple threeawn), Buchloe dactyloides (Nutt.) Engelm. (buffalograss), and Schizachyrium scoparium (Michx.) Nash (little bluestem). Aboveground, belowground, and total biomass measures of these grasses were analyzed over the five different DU soil concentrations of 0, 50, 500, 5,000, and 25,000 mg kg‐1. Differential plant response to DU was observed between the three grasses, wiith A. purpurea and B. dactyloides exhibiting a threshold‐type response in which no changes in biomass occurred until the highest treatment level of 25,000 mg kg‐1 was reached. Schizachyrium scoparium, however, demonstrated a large degree of growth stimulation, or hormesis, at the 50 and 500 mg kg‐1 DU concentrations. Several possible mechanisms of this hormetic response are offered.

Nitrogen availability and fungal‐bacterial responses in successional semiarid steppe soils

Nitrogen availability plays a major role in influencing the microbial community structure as plant‐soil systems develop in succession. In this study, three successional sites, sampled 5, 13, and 39 years after disturbance, were compared with an undisturbed native site. Microbial responses were related to time since disturbance and soil nitrogen (N) availability. Nitrogen was modified by mineral N and carbohydrate additions. Microscopic techniques were used to assess the biomass (total and active) of bacteria and fungi at spring and summer samplings. In the undisturbed native plots, with decreased N availability, increases occurred in microscopically assessed microbial biomass, primarily fungal, in comparison with control plots. This fungal response did not occur on the previously disturbed successional plots that were treated in a similar manner. Ratio analyses of the various parameters provided additional information related to these responses. For both samplings, the ratio of total fungi/total bacteria ...

Uptake of Munitions-Derived Depleted Uranium by Three Grass Species

Abstract Uptake of depleted uranium (DU, 238U) derived from weathered munitions was assessed in a greenhouse experiment utilizing three common grass species, Schizachyrium scoparium (little bluestem), Buchloe dactyloides (buffalograss), and Aristida purpurea (purple threeawn). Both aboveground and belowground uptake was dependent on the soil DU concentration, and on the experimental moisture regime utilized during the experimental duration. Uptake was enhanced under higher moisture regimes, suggesting a greater degree of DU solubility and concomitant plant availability. Concentration ratios (calculated by dividing plant tissue DU concentrations by soil DU concentrations) decreased with increasing soil DU concentrations, but increased as more moisture was applied. The toxicity level of DU in root tissue was estimated to 270 mg kg−1.

Effects of soil fumigation and seeding regimes on secondary succession in a semiarid shrubland.

In order to ascertain the degree to which disturbance and subsequent recovery of belowground biotic components (mycorrhizal and decomposer systems) influence successional dynamics, a portion of semiarid shrubland in northwest Colorado was mechanically disturbed in 1984. Two treatments, soil fumigation (fumigated with methyl bromide and not fumigated) and seeding (early-seral species, late-seral species, and not seeded) were imposed on 500 m2 plots in each of four replications. Aboveground annual production by species, and decomposition rates were measured in 1991, 7 years after the initial disturbance and treatments. Mycorrhizal infection potential had been previously measured at this site from 1984-1988. Seeding to late-seral species resulted in greater production rates (and greater community composition) of late-seral species, whereas seeding to early-seral species had only a minor effect when compared to unseeded plots 7 years after disturbance. Fumigation slowed the rate of succession on unseeded plots, primarily by decreased production of perennial grasses. Mycorrhizal infection potential, initially greatly reduced by the disturbance and by fumigation, recovered to near-background levels on unfumigated plots but not on fumigated plots after 4 years. Decomposition experiments suggest that seeding may have facilitated recovery of the decomposer system after initial disturbance and fumigation. Our results suggest that the redevelopment of soil biotic communities following disturbance has a significant impact on successional dynamics, particularly the replacement of early-seral annuals by mid-seral perennial grasses.