Tetsuo Masahashi

Determination of serum Δ5-3β-hydroxysteroid sulphates by combined high-performance liquid chromatography and immobilized 3β,17β-hydroxysteroid dehydrogenase in column form

Abstract We studied the use of an immobilized enzyme, covalently bound to aminopropyl-CPG, in the analysis of individual Δ 5 -3β-hydroxysteroid sulphates. A microcolumn with immobilized 3β,17β-hydroxysteroid dehydrogenase was prepared and used together with high-performance liquid chromatogaphy (HPLC). The reduced nicotinamide-adenine dinucleotide produced from Δ 5 -3β-hydroxysteroids by this enzyme was fluorimetrically determined. The immobilized enzyme was sufficiently stable for at least one month or for 180 tests when used repeatedly. A clinical trial demonstrated that this HPLC—immobilized enzyme method is superior to the soluble enzyme method, giving reliable and reproducible results at a low cost.

Which infertile women should be indicated for sonohysterography

To evaluate the indications for transvaginal saline contrast sonohysterography (TV‐SCSH) in endometrial screening by transvaginal sonography in infertile women.

Premature Ovarian Failure in a Woman with 47,XXX Karyotype following Gonadotrophin Treatment

ABSTRACT A woman resulted in premature ovarian failure following gonadotrophin treatment for secondary sterility is described. The state of ovarian failure was confirmed by increased serum gonadotrophin titers, decreased serum estrogen levels, and lack of ovarian follicles on histological examination. The cause of premature ovarian failure was investigated and 47,XXX karyotype was found in chromosomal analysis. The role of sex chromosome abnormalities in the pathogenesis of this entity is discussed. Key words: premature ovarian failure, 47,XXX, gonadotrophin treatment