Thiago Carvalho da Silva

Agronomic divergence of sorghum hybrids for silage yield in the semiarid region of Paraiba

The objective of this study was to evaluate the agronomic divergence of 25 sorghum hybrids (Sorghum bicolor L. Moench) in the semiarid region of Paraiba. A randomized block design with three replications was used for evaluation of plant height (PH), total natural matter production (TNMP) and total dry matter production (TDMP) and the percentage of components of DM (panicle, leaf blade, stem and dead matter) of the following hybrids: 866005, 866019, 866033, 866034, 866035, 866036, 866037, 866040, 866041, 866042, 866043, 866044, 870025, 870031, 870035, 870041, 870051, 870067, 870081, 870085, 870095, 1F305, BRS 610, Volumax, and XBS60329. Hybrid 1F305, followed by hybrid 866034, presented the highest average PH. There was a range from 7.679 to 20.948 kg/ha (average of 13,799 kg/ha) for TDMP. Hybrids 1F305, BRS 610 and Volumax presented less potential, and hybrids 866,041 and 866,042 were the most productive. Based on cluster analysis and subjective cut in 50% of dissimilarity, it was possible to establish four hierarchical groups, from which two stood out concerning productive characteristics. The group formed by hybrids Volumax, BRS 610, and XBS60329 presented lower averages for yield and lower percentage of panicle. Hybrids 866041 and 866042 show a higher total dry matter production, with values around 20,000 kg/ha.

Microbial populations, fermentative profile and chemical composition of signalgrass silages at different regrowth ages

Microbial populations, pH, ammonium nitrogen/total nitrogen (N-NH3) ratio, concentrations of lactic acid, acetic acid and butyric acid and the chemical composition of Brachiaria decumbens cv. Basiliski silages at different regrowth ages (30, 40, 50, 60 and 70 days) were evaluated by using 2-kg capacity laboratory silos. It was used a 5 × 6 factorial scheme (5 regrowth ages × 6 fermentation periods) in a complete random design, with three replicates. The fermentation periods were 1, 3, 7, 14, 28 and 56 days. Lactic acid bacteria populations were recorded in fresh forage varying from 3.93 (30 days of regrowth) to 5.51 (70 days of regrowth) log colony-forming units (cfu)/g forage. Maximum populations of these microorganisms were recorded in the silages on the seventh day of fermentation (8.69 log cfu/g silage). Enterobacteria populations persisted until the 28th day, with maximum values found as early as the first day of fermentation (7.89 log cfu/g silage). Levels of DM, NDF, ADF and ADIN increased linearly whereas values of CP decreased linearly with age of regrowth of the plants. There was a linear reduction in the levels of DM, CP and NDF and linear increase in the levels of ADIN over the period of fermentation. N-NH3 level decreased and increased linearly with regrowth age and fermentation period, respectively. The pH decreased exponentially with fermentation period. Lactic acid increased and butyric acid decreased linearly with regrowth age. The predominant Lactobacillus plantarum species in signalgrass plants is Lactobacillus plantarum.

Lactic Acid Bacteria in Tropical Grass Silages

The basic principle silage is to store the surplus forage keeping its stability and nutritional value until it is required to feed the animals. This process takes place in anaerobic conditions, where the lactic acid produced by the LAB inhibits the proliferation of spoilage microorganisms, which are less tolerant to acidic conditions. Thus, as the pH values decline, the silage losses decline as well due to the greater conversion of plant soluble carbohydrates (the main substrate for LAB) in lactic acid, with 96.9% rate of energy recovery (Mc Donald et al., 1991). The major soluble carbohydrates present in forage crops are fructose, glucose, sucrose and frutosanas, according to Woolford, (1984), sucrose and frutosanas are rapidly hydrolyzed in their monomers at forage harvest.

Microbial and fermentation profiles, losses and chemical composition of silages of buffel grass harvested at different cutting heights

The present study evaluated the microbial population, fermentation profile, losses and dry matter recovery, and chemical composition of silages of buffel grass at different cutting heights. To evaluate the microbial fermentation dynamics, the treatments resulted from a 4 × 5 factorial combination consisting of 4 cutting heights and 5 fermentation periods, in a completely randomized design with three replications. The fermentation was evaluated at the end of 1, 3, 7, 15 and 30 days. The other characteristics of silages with 30 days were evaluated following a completely randomized design with four treatments, consisting of 4 cutting heights (30, 40, 50 and 60 cm), and five replications. Fermentation period and cutting height effects and interaction between both factors were observed on the populations of lactic acid bacteria (LAB), enterobacteria and molds and yeasts. The peak of development of LAB populations was observed on the seventh day of fermentation for the heights of 40 and 50 cm, with 8.25 and 8.30 log cfu/g, respectively. The pH values of silages ranged with different cutting heights, in which at the height of 50 cm the decrease was most pronounced. However, the pH values were similar between the cutting heights at the end of 30 days of fermentation. Quadratic relationship was observed between lactic acid concentrations and cutting heights. The crude protein content behaved linearly, initially showing 128.5 g/kg DM at 30 cm, decreasing as the cutting heights increased. The neutral detergent fiber and ether extract contents increased linearly with the cutting heights. Based on microbial populations, fermentation, losses and chemical composition, it is recommended to harvest buffel grass for silage from 50 cm on.

Agronomic Evaluation of Sorghum Hybrids for Silage Production Cultivated in Semiarid Conditions

The aim of this study was to study the agronomic traits of different Sorghum bicolor (L.) Moench hybrids for silage productionin semiarid conditions. It was a 1-year evaluation conducted in a randomized block design with 24 treatments and three replicates. The treatments were sorghum hybrids developed by the breeding program of “Embrapa Milho e Sorgo” (Brazilian Agricultural Research Corporation). The fresh matter yield (FMY) in the first cut varied from 22,643.56 to 44,033.15 kg/ha, with an average of 32,607.37 kg/ha, leading to the formation of two groups. Similar results were observed for the dry matter yield (DMY), in which the highest group yielded from 9,471.32 to 14,540.23 kg/ha dry matter (DM). For plant regrowth, there was an increase in the number of stems and a decrease in the amount of panicles. Two groups were formed for the accumulated dry matter yield (ADMY), averaging 14,217.91 kg/ha; the highest group showed mean values of 18,003.00 to 14,221.94 kg/ha. The evaluated sorghum hybrids exhibited satisfactory accumulated forage yields due to their high yield in regrowth, which indicates that they are suitable for use in animal production systems in semi-arid regions.

Cinética de fermentação ruminal in vitro de silagens de híbridos de sorgo

The aim of this research was to evaluate the silage of 23 sorghum hybrids using the in vitro semi-automatic gas production technique. A completely randomized design with 23 treatments was used, with three replications, and treatments were the sorghum-hybrids silages. Hybrids were grown at the experimental station of the Instituto Nacional do Semiarido (INSA), in Campina Grande - PB. The gas production was estimated using the bicompartimental logistic model, adjusted to the curves of cumulative gas production. A significant effect (P<0.05) was observed for final gas of fibrous carbohydrates (VfCF), which results ranged from 105.99 to 144.53mL/g of DM. Degradation rate of fibrous carbohydrates (KdCF) values ranged (P<0.05) for the hybrid 866041, which showed the highest result 0.015h-1. Final total volume (VFT) was affected by hybrid (P<0.05), in which hybrids 870085, Volumax and XBS60329 had the highest (P<0.05) values compared to other hybrids. By multivariate analysis were formed six distinct groups. Group 2, consisting of hybrids 22 (Volumax) and 23 (XBS60329) showed the highest average values for gas volumes, both for fibrous carbohydrates and for the non-fibrous. Group 4, comprising the hybrid 1 (866005), had the lowest gas volume produced means and a highest lag time (3.15 hours). All hybrids studied have potential to silage, according to the ruminal fermentation kinetics.

Divergence of the fermentative and bromatological characteristics of 25 sorghum hybrid silages

The objective of this study was to evaluate the divergence of bromatological and fermentative characteristics of silages of 25 sorghum (Sorghum bicolor L. Moench) hybrids. The experimental design was completely randomized with 25 treatments and four replications. The mean values of pH differed with variation from 3.45 to 3.87 for hybrids 866005 and 870051, respectively, and the mean pH value was 3.66. The average percentage of lactic acid ranged from 2.90 to 7.43 dag/kg of dry matter (DM) in hybrids 870035 and 866005, respectively, with an average of 4.65 dag/kg DM. Through cluster analysis by the Tocher method and average Euclidean distance based on the fermentative characteristics, five groups were formed: one with 18 hybrids, another with four and three groups with one hybrid in each. In group three, there were the hybrids that showed the highest values of lactic acid. Hybrids 870025, 870035, 870081, 870095, 866034, and 870031 showed higher DM content and the hybrid BRS 610 had the lowest average compared with the others. Hybrids 1F305, BRS 610, Volumax, and XBS60329 had the lowest levels of non-fiber carbohydrates and higher neutral detergent fiber corrected for ash and protein. Despite the fermentative and bromatological divergences between hybrids, they are not a limiting factor to silage production.

Evaluation of whey fermented by Enterococcus faecium in consortium with Veilonella parvula in ruminant feeding

ABSTRACT - The objective of this study was to evaluate the whey fermented by Enterococcus faecium in consortiumwith Veilonella parvula on the in vitro growth of ruminal bacteria and as a supplement in the cattle diet. In the in vitro experiment, a randomized design, with the following combinations was used: ruminal bacteria; ruminal bacteria and inactivewhey; ruminal bacteria and active whey; and active whey. In the in vivo experiment, five fistulated Zebu Holstein-Zebu crossbredheifers were distributed in a 5 × 5 Latin square. Supplements were formulated without the addition of whey, with the additionof two levels of unfermented whey (2.5 and 5 L/day) or two levels of fermented whey (2.5 and 5 L/day). A positive effectof the whey fermentation was detected on the consumption of dry matter, organic matter, crude protein, ether extract, non-fiber carbohydrates and neutral detergent fiber, corrected for ash and protein in kg/day. No effects of whey were observed onthe pH and concentration of rumen ammonia nitrogen, serum concentration of urea and glucose, urinary excretion of ureaor nutrient digestibility, except for the total digestible nutrients. Supplementation with whey improved the apparent nitrogenbalance, but supplementation with fermented whey decreased the intestinal flow of microbial nitrogen and microbial synthesisefficiency in relation to the unfermented whey. The whey fermentation process does not optimize the physiological responsesof heifers supplemented with 2.5 and 5.0 L of whey.Key Words: lactate, organic acids, propionic acid, rumen

Características morfogenéticas e produção do capim buffel adubado com digesta bovina sólida

The objective was to evaluate the morphogenesis and the production of biomass of buffel grass fertilized with different amounts of bovine solid digesta. We used an experimental area of two hectares of grazing buffel grass (Cenchrus ciliaris cv. Molopo) deployed in 2006, this area is designed exclusively for the production of hay. In 2009 the experiment was implemented a total area of 375m², which was divided into 25 plots of 15m2 each, with each plot had 8m2 (4x2) of the experimental area and a boundary line of 0.5m wide around the perimeter. We used a split-plot in time, in randomized complete block experimental design, with five treatments and five repetitions of the corresponding amounts of bovine digesta (3.5; 7.0; 10.0 and 13.5t/ha), totaling 25 experimental units. There was interaction between the amounts of digesta and cuts for the production of green biomass, and for all quantities of digesta were obtained higher yields applied to the third cut. The largest green biomass production was obtained for treatment with 13.5t/ha of digesta. There was no interaction between cuts and fertilization with digesta to the number of live tillers, number of dead tillers, number of live leaves per tiller, phyllochron, height and diameter of clump. Thus, it is recommended the application of bovine digesta to be efficient in increasing the production of biomass of buffel grass cv. Molopo, through changes in some structural features, can be used as organic fertilizer in pastures of grass.

Importance of the Fermentation to Produce High-Quality Silage

The objective of this chapter was to discuss the importance of the fermentation processes for silage making and how it affects the final quality of the silage. The preservation of the forage crops as silage is based on a fermentation process that lows the pH and preserves the nutritive value of the fresh crop. The main principle is the production of lactic acid by the lactic acid bacteria from the metabolism of the water-soluble carbohydrates in the fresh crop. However, different fermentations may occur into the silo environment and it depends on the availability of substrate, the microbial populations, the moisture content, and the buffering capacity of the crop at the ensiling. The fermentation is quite important in the ensiling process because it affects the nutritional quality of the silage and the animal performance. If the fermentation does not occur as recommended and the undesirable fermentations will take place, which will result in a total spoiled feed that is potentially risky for animals and human’s health. Well-fermented silage can be used in diets for ruminant animals without any risk for their health and without compromise the productive performance.