Tohru Masuda

DNA ploidy of colorectal carcinoma: correlation with conventional prognostic variables

The DNA content of tumor cells was determined in 51 cases of colorectal carcinoma and correlated with conventional clinicopathologic features. Normal diploid content of DNA was found in cells from 19 tumors, and aneuploidy was found in cells from 32 tumors. An aneuploid stemline of tumor cells appeared to be associated with advanced disease. However, a statistical analysis using Pearsons chi-square test disclosed no significant correlation between DNA ploidy of tumor cells and conventional clinicopathologic characteristics of colorectal carcinoma, such as differentiation, staging, lymph node status, and depth of invasion.

Chronic idiopathic intestinal pseudo-obstruction caused by acquired visceral neuropathy localised in the left colon: report of two cases

Two cases of chronic idiopathic intestinal pseudo-obstruction (CIIP) are reported. One is a 51-year-old man, and the other is a 47-year-old woman. Both patients presented with severe constipation and barium enema showed a marked dilation of the right colon, and a narrowing in the left colon. Studies done on the motility of the colon and anorectum revealed normal resting pressure profiles of the anorectom, a normal recto-anal reflex, and a normal resting tone of the collapsed colon. Administration of methacholine chloride, however, provoked large, non-propulsive movements in the collapsed colon, which were inhibited by the administration of atropin sulfate. Histologic examination disclosed a marked decrease in neurons and an increase of Schwann cells in the myenteric plexus of the collapsed colon. CIIP due to acquired visceral neuropathy localised in the left colon, was diagnosed as a result of manometric and histologic findings. One case was cured surgically, by a left hemi-colectomy, and the other was cured medically using choline antagonists and laxatives.

A case report of scirrhous hepatocellular carcinoma.

症例は69歳男性で上腹部鈍痛, 食欲不振を主訴として入院.腹部超音波検査と腹部CT検査にて肝S5に径7cm大の腫瘍を認め, 腹部血管造影では動脈門脈ともに前下区域枝の偏位を認めた.術前診断は胆管細胞癌であった.手術は拡大右葉切除を行った.病理組織では腫瘍組織は厚い線維性間膜で囲まれ硬化型肝細胞癌と診断された.DNA histogramより腫瘍はaneuploidを示し, 胆管細胞癌に特異的なTPA染色は陰性であった.本例は術後8か月と早期に骨転移をきたし死亡し悪性度の高い腫瘍と考えられた.

Correlation between serum immunosuppressive substance and clinico-pathological findings in patients with gastric carcinoma

Serum levels of immunosuppressive substance (IS) were determined in 99 patients with gastric cancer and in 32 healthy individuals. The serum IS levels in the patients (769.6±314.8 μg/ml) were significantly higher than those in the healthy individuals (549.7±104.7 μg/ml). A multivariate analysis on the correlation between serum IS levels and clinicopathological findings in the patients disclosed that there was a close correlation between the serum IS levels and the depth of invasion, in particular, the prognostic serosal invasion, metastasis to the distal lymph nodes and peritoneal dissemination. There was, however, no correlation between the serum IS level and hepatic metastasis. Serum IS levels were higher in patients with well-differentiated adenocarcinoma than in those with poorly differentiated adenocarcinoma or signet ring cell carcinoma. A serum IS level higher than 1000 μg/ml indicates the possibility that the tumor is only palliatively resectable because of involvement of the distal lymph nodes or peritoneal dissemination.

Histopathological changes, mucin production and cellular DNA content in rectal mucosa of patients with ulcerative colitis: A comparison between findings in adults and adolescents

Histopathological examination, specific mucin staining and analysis of cellular DNA content of rectal mucosa were carried out in twenty-eight tissue specimens biopsied from ten adult and eight adolescent patients with ulcerative colitis. There was an increase in proliferative activity in the rectal mucosa in both groups with active colitis, but, there were no significant differences in the potential for malignant transformation of rectal mucosa in these groups.