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Dive into the research topics where Tommy Nordström is active.

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Featured researches published by Tommy Nordström.


Biotechnology and Applied Biochemistry | 2000

Direct analysis of single‐nucleotide polymorphism on double‐stranded DNA by pyrosequencing

Tommy Nordström; Mostafa Ronaghi; Lena Forsberg; Ulf de Faire; Ralf Morgenstern; Pål Nyrén

Pyrosequencing, a new method for DNA sequencing, is gaining widespread use for many different types of DNA analysis. The method takes advantage of four coupled enzymes in a single tube assay to monitor DNA synthesis in real time using a luminometric detection system. Here, we demonstrate the use of pyrosequencing for direct analysis of single‐nucleotide polymorphism on double‐stranded PCR product. Pyrosequencing data on the human glutathione peroxidase gene (GPX1) from several individuals were analysed and three different allelic variants were determined and confirmed. The possibility of further simplifying the sequencing and template‐preparation steps is discussed.


Analytical Biochemistry | 2002

Long-read pyrosequencing using pure 2'-deoxyadenosine-5'-O'-(1-thiotriphosphate) Sp-isomer.

Baback Gharizadeh; Tommy Nordström; Afshin Ahmadian; Mostafa Ronaghi; Pål Nyrén


Analytical Biochemistry | 2000

Method Enabling Pyrosequencing on Double-Stranded DNA

Tommy Nordström; Karim Nourizad; Mostafa Ronaghi; Pål Nyrén


Electrophoresis | 2004

Pyrosequencing trade mark technology at elevated temperature.

Jonas H.C. Eriksson; Baback Gharizadeh; Tommy Nordström; Pål Nyrén


Analytical Biochemistry | 2001

Method enabling fast partial sequencing of cDNA clones

Tommy Nordström; Baback Gharizadeh; Nader Pourmand; Pål Nyrén; Mostafa Ronaghi


Analytical Biochemistry | 2003

Method enabling firefly luciferase-based bioluminometric assays at elevated temperatures

Jonas Eriksson; Tommy Nordström; Pål Nyrén


Analytical Biochemistry | 2004

Improvements in Pyrosequencing technology by employing Sequenase polymerase.

Baback Gharizadeh; Jonas Eriksson; Nader Nourizad; Tommy Nordström; Pål Nyrén


Journal of Biochemical and Biophysical Methods | 2002

Method for one-step preparation of double-stranded DNA template applicable for use with Pyrosequencing™ technology

Tommy Nordström; Anders Alderborn; Pål Nyrén


BioTechniques | 1999

Real-time bioluminometric method for detection of nucleoside diphosphate kinase activity.

Samer Karamohamed; Tommy Nordström; Pål Nyrén


Archives of Industrial Biotechnology | 2017

Method enabling detection of single-stranded DNA ligation activity.

Tommy Nordström; Pål Nyrén

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Pål Nyrén

Royal Institute of Technology

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Baback Gharizadeh

Royal Institute of Technology

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Jonas Eriksson

Royal Institute of Technology

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Afshin Ahmadian

Royal Institute of Technology

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Anna Svantesson

Royal Institute of Technology

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Karim Nourizad

Royal Institute of Technology

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Nader Nourizad

Royal Institute of Technology

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