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Dive into the research topics where Tonny Johnson is active.

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Featured researches published by Tonny Johnson.


Methods of Molecular Biology | 2008

Methods for the purification of HQ-tagged proteins.

Becky Godat; Laurie Engel; Natalie A. Betz; Tonny Johnson

The HQ (H = histidine, Q = glutamine) tag is a small fusion tag that can be isolated using immobilized metal affinity columns. HQ-tagged proteins can be expressed and purified from bacterial cells under native and denaturing conditions, mammalian cells, insect cells, wheat germ and rabbit reticulocyte. Furthermore, HQ-tagged proteins can be purified using magnetic or non-magnetic resins in multiple formats from small to large-scale and manual or automated. In this chapter, we have described various protocols for the purification of HQ-tagged proteins.


Archive | 2011

Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule

Aldis Darzins; Lance P. Encell; Tonny Johnson; Dieter Klaubert; Georgyi V. Los; Mark McDougall; Keith V. Wood; Monika G. Wood; Chad Zimprich


Archive | 2004

Substrates for covalent tethering to proteins

Aldis Darzins; Lance P. Encell; Tonny Johnson; Dieter Klaubert; Georgyi V. Los; Mark McDougall; Keith V. Wood; Monika G. Wood; Chad Zimprich


Archive | 2003

Methods for separating molecules

Daniel J. Simpson; Tonny Johnson; John Shultz; Roderick G. Flemming; Rebecca Godat; Sanchayita Kar


Archive | 2003

Compositions for separating molecules

Daniel J. Simpson; Tonny Johnson; Roderick G. Flemming


Archive | 2006

Device and method for separating molecules

Laurie Engel; Tonny Johnson


Archive | 2005

Methods and kits for purifying his-tagged proteins

Tonny Johnson; Rebecca Godat; Laurie Engel


Collaborative Innovation in Drug Discovery | 2014

Open Source Drug Discovery for Neglected Diseases

Tonny Johnson; Sanchayita Kar


Archive | 2005

Kovalentes Anbinden von Funktionsgruppen an Proteine und Substrate daf

Aldis Darzins; Lance P. Encell; Tonny Johnson; Dieter Klaubert; Georgyi V. Los; Mark McDougall; Keith V. Wood; Monika G. Wood; Chad Zimbrich


Archive | 2005

Kovalentes anbinden von funktionellen gruppen an proteine und substrate dafür

Aldis Darzins; Lance P. Encell; Tonny Johnson; Dieter Klaubert; Georgyi V. Los; Mark McDougall; Keith V. Wood; Monika G. Wood; Chad Zimprich

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