Toshihiro Morita

Immunological response of regional lymph nodes after tumor cryosurgery: Experimental study in rats

Seven days after inoculation of metastasizing rat mammary tumor No. 1 into the thigh of 5-week-old female Sprague-Dawley rats, the tumor was treated cryosurgically by two-cycle freezing and by contact methods at -170 degrees C. Weights of the thymus and the spleen, histological findings of the lumbar lymph nodes, phytohemagglutinin (PHA)-induced blastogenesis of lymphocytes obtained from the lumbar lymph nodes and peripheral blood, and resistance rate to tumor rechallenge were examined 1, 3, 6, 10, and 17 week(s) after cryosurgery, with the following results: Thymus weight gradually decreased by 3 weeks after cryosurgery, while spleen weight increased by 1 week, recovering the preoperative level at 6 weeks. Paracortical hyperplasia of the lumbar lymph nodes markedly increased in 1 week and sinus histiocytosis increased after 3 weeks, both remaining at high values until 10 weeks, while germinal center hyperplasia showed a high value at 3 weeks and thereafter decreased gradually. PHA-induced blastogenesis of the lumbar lymph nodes significantly increased 1 week after cryosurgery and remained at its high value until 10 weeks. PHA-induced blastogenesis of peripheral lymphocytes showed the lowest value at 3 weeks and then significantly increased at 6 weeks. Resistance rate to rechallenge showed the lowest value at 3 weeks, reaching the highest level 10 weeks after cryosurgery. From the above results, it was suggested that anti-tumor immunity (resistance to tumor rechallenge) induced by cryosurgery was at the lowest level at 3 weeks after cryosurgery, and gradually increased starting at 6 weeks.(ABSTRACT TRUNCATED AT 250 WORDS)

A Case Report of Crohn's Disease of the Appendix.

虫垂に限局したCrohn病は非常にまれな疾患で, 本邦では10例の報告をみるにすぎない. 今回, われわれは虫垂Crohn病と診断された1例を経験したので報告する. 症例は20歳の男性, 右下腹部痛を主訴に当科を受診した. 受診時, 右下腹部に圧痛, 筋性防禦を認めた. 腹部超音波, 腹部CTで回盲部に腫大した腫瘤陰影を認めた. 急性虫垂炎と診断し, 緊急開腹術を施行した. 虫垂は炎症性に著明に腫大しており, 35×30mmの大きさであった. 虫垂腫瘍と診断し, 2期的にD2リンパ節郭清を伴う回盲部切除を施行した. 術後の病理組織検査で, 虫垂に全層性炎症性病変, 非乾酪性小型肉芽腫および裂溝が認められ, 虫垂Chohn病と診断された. 術後経過は順調で, 2年経った現在, 再発の徴候は認めていない.