Toshio Anmo

High-performance anion-exchange chromatography of ultraviolet-absorbing constituents of human urine.

A 100-microliters urine sample was chromatographed on a column packed with a strongly basic macroreticular anion-exchange resin (Diaion CDR-10,5--7 micrometers with a nominal 35% cross linkage). The elution was performed with a linear acetate gradient from 0 to 6.0 M at an average flow-rate of 0.72 ml/min and at an average pressure of 104 kg/cm2. The relative standard deviation of retention times and peak height was +/- 4% or less. The properties of the macroreticular anion-exchange resin, the effect of the particle size, the pH of acetate buffers, and the effect of the flow-rate of the eluent on the separation were investigated. Thirty three components of urine were then resolved and named.

High-Resolution Anion-Exchange Chromatography of Ultraviolet-Absorbing Constituents of Human Erythrocytes

Abstract The analysis of nucleic acid components of human erythrocytes was achieved by high-resolution anion exchange chromatography using a column packed with a macroreticular anion-exchange resin and linear gradient elution with ammonium acetate solution, pH 4.5. On the chromatogram of trichloroacetic acid extracts from human erythrocytes, at least 50 components were detected as ultraviolet-absorbing constituents. On assignment of the chromatographic peaks they were found to be nucleic acid components. The analysis was achieved in 120 min and the elution time of guanosine 5′-triphosphate was 64.3 min.